Mustafa Tolga TOLON, Ulviye KARACALAR, Caner ŞİRİN

Observation of Vibrio mediterranei (Pujalte and Garay 1986) / Vibrio shiloi (Kushmaro et al. 2001) bacteria from skin ulcers of the cultured sea cucumber Holothuria poli (Delle Chiaje, 1823)

Skin ulcer syndrome is frequently reported as a serious disease affecting the health, growth and mortality of stocks in sea cucumber aquaculture. In this study, bacteria isolated predominantly from skin ulcers of sea cucumber Holothuria poli (Delle Chiaje, 1823), a new candidate for aquaculture in the Mediterranean, were investigated. Morphological and biochemical tests, and molecular analysis methods were used to examine the dominant bacteria in the lesions of H. poli showing skin ulceration, peristome tumour and visceral ejection symptoms in rearing tanks. Present study is the first report for isolation and identification of Vibrio mediterranei (Pujalte and Garay 1986) (called also Vibrio shiloi Kushmaro et al. 2001) as a predominant gram-negative bacterium in the skin ulcers of H. poli. Reference data provided from the present study would lead to understand possible major pathogens causing skin ulceration syndrome and is crucial for the prophylaxis and treatment of such disease in holothuriculture.

Kültürü yapılan Holothuria poli (Delle Chiaje, 1823) türü deniz hıyarının deri ülserlerinden Vibrio mediterranei (Pujalte ve Garay 1986) / Vibrio shiloi (Kushmaro vd. 2001) bakterisinin gözlenmesi

Deri ülseri sendromu deniz hıyarı yetiştiriciliğinde stokların sağlığını, büyümesini ve ölüm oranını etkileyen ciddi bir hastalık olarak sıklıkla rapor edilmektedir. Bu çalışmada, Akdeniz'de yetiştiricilik için yeni bir aday olan Holothuria poli (Delle Chiaje, 1823) türü deniz hıyarının deri ülserlerinden baskın halde izole edilen bakteriler incelenmiştir. Yetiştirme tanklarında deri ülseri, peristomal tümör ve bağırsak çıkarma semptomları gösteren deniz hıyarlarının deri lezyonlarındaki dominant bakterileri incelemek amacıyla morfolojik ve biyokimyasal testler, ve moleküler analiz yöntemleri kullanılmıştır. Bu çalışma, H. poli'nin cilt ülserlerinde dominant gram-negatif bir bakteri olarak Vibrio mediterranei (Pujalte ve Garay 1986) 'nin (Vibrio shiloi Kushmaro vd. 2001 olarak da adlandırılır) izolasyonu ve tanımlanması konusunda ilk rapordur. Bu çalışmadan sağlanan referans veriler, deri ülseri sendromuna neden olan olası baskın patojenlerin anlaşılmasına yardımcı olurken deniz hıyarı kültüründe bu tür hastalıkların önlenmesi ve tedavisi için yol gösterici olması yönünden önemlidir.

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