Hasan ULUSAL, Mahinur KIRICI, Mehmet Reşit TAYSI, Seyithan TAYSI, Muammer KIRICI, Bünyamin SÖĞÜT

Civa klorür (HgCl2) uygulanan gökkuşağı alabalığı (Oncorhynchus mykiss) karaciğer dokusunda nitrozatif ve oksidatif stresin rolü

Çalışmanın amacı, gökkuşağı alabalığı (Oncorhynchus mykiss) karaciğer dokusunda civa klorürün (HgCl2) neden olduğu oksidatif stresi belirlemektir. Bu amaçla gökkuşağı alabalığı üzerine HgCl2’nin LD50 değeri 551 μg/L olarak tespit edilmiştir. Çalışmada, her grupta 10 balık (n=10) olacak şekilde dört grupta toplam 40 adet balık 2 ve 7 gün süreyle HgCl2’ün iki subletal dozun %25' ine ve %50' sine (138 ve 276 μg/L) maruz bırakılmıştır. Oksidatif stresi belirlemek için peroksinitrit (ONOO-), toplam oksidan seviyesi (TOS), toplam antioksidan seviyesi (TAS), oksidatif stres indeksi (OSİ) ve malondialdehit (MDA) tayinleri yapılmıştır. Çalışma sonucunda, genel olarak karaciğer dokularındaki ONOO- ,TOS, TAS ve OSİ düzeyleri açısından gruplar arasındaki fark istatistiksel olarak önemli (P0.05) çıkmıştır. Sonuç olarak HgCl2’nin karaciğer dokusunda ONOO-, TOS, TAS, OSİ ve MDA düzeylerini arttırdığı ve civanın küçük dozlarının bile balıklar için toksik olduğu belirlenmiştir.

The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

The aim of this study was to determine oxidative stress caused by mercury chloride (HgCl2) in rainbow trout (Oncorhynchus mykiss) liver tissue. For this purpose, the LD50 value of HgCl2 on rainbow trout was determined as 551 μg/L. In the study, 40 fish in four groups were exposed to 25% and 50% (138 and 276 µg/L) of the two subletal doses of HgCl2 for 2 and 7 days, with 10 fish (n=10) in each group. To determine oxidative stress; peroxynitrite (ONOO−), total oxidant level (TOS), total antioxidant level (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) were analyzed. In the study, it was observed that the differences between the groups in terms of ONOO−, TOS, TAS and OSI levels in the liver tissues was significant (P0.05) in terms of MDA values. As a result, it can be concluded that HgCl2 increases ONOO−, TOS, TAS, OSI and MDA levels in liver tissue and even small doses of mercury are toxic to fish.

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