Karyotype, C-band and NOR phenotype of Anatolian endemic fish Squalius anatolicus (Bogutskaya, 1997) (Teleostei, Leuciscidae)

The karyotype and distribution of constitutive heterochromatin and nucleolus organizer regions (NORs) of Anatolian leuciscine endemic to Lake Beysehir, Squalius anatolicus (Bogutskaya, 1997) were analyzed respectively using conventional Giemsa-staining, C-banding and Ag-impregnation. Diploid chromosome number was 2n = 50 and karyotype consisted of 7 pairs of metacentric, 13 pairs of submetacentric, 5 pairs of subtelo- to acrocentric chromosomes, NF value equaled 90. Heteromorphic elements indicating sex chromosomes were not detected. C-banding revealed clear pericentromeric constitutive heterochromatin blocks in several chromosomes. Ag-impregnation revealed the size heteromorphism of NORs that covered almost the entire short arms of the middle-sized submetacentric chromosome pair. The karyotype pattern and simple NOR phenotype of S. anatolicus are nearly identical with that found not only in Squalius species analyzed to date but also in many other representatives of the Eurasian leuciscine cyprinids, which indicates remarkable chromosome stasis in this leuciscid lineage.

Anadolu’ya endemik Squalius anatolicus (Bogutskaya, 1997) (Teleostei, Leuciscidae) türünün karyotip, C-bant ve NOR fenotipi

Beyşehir Gölü’ne endemik Anadolu leuciscini Squalius anatolicus (Bogutskaya, 1997)’un karyotipi, konstitütif heterokromatin dağılımı ve çekirdekçik organize edici bölgeleri (NOR’lar) sırası ile geleneksel Giemsa-boyama, C-bantlama ve Gümüş emdirme teknikleri kullanılarak analiz edilmiştir. Diploid kromozom sayısı 2n = 50 olmak üzere; karyotipinin 7 çift metasentrik, 13 çift submetasentrik, 5 çift subtelo – akrosentrik kromozomdan oluştuğu ve toplam kol sayısının 90 olduğu tespit edilmiştir. Cinsiyet kromozomlarını temsil eden heteromorfik yapılar gözlenmemiştir. C-bantlama birçok kromozomda belirgin perisentromerik heterokromatin blokları ortaya çıkarmıştır. Gümüş emdirme ile orta boy submetasentrik kromozom çiftinin neredeyse kısa kolunun tamamını kaplayan NOR’ların büyüklük heteromorfizmi tespit edilmiştir. S. anatolicus’un karyotip şekli ve temel NOR fenotipi sadece bugüne kadar analiz edilen Squalius türlerinde değil, aynı zamanda bu leuciscid soyunda büyük ölçüde kromozom durumunu gösteren Avrasya leuciscinlerinin diğer birçok temsilcisinde bulunanla hemen hemen aynıdır.

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