Karadeniz'de yaşayan Pisi Balığı'nın Platichthys flesus luscus (Pallas, 1814) (Pisces, Pleuronectiformes) ilk karyolojik tanımlaması

Bu çalışmada, Karadeniz'de yaşayan yassı balık türlerinden (Pleuronectiformes) birisi olan Platichthys flesus luscus (Pallas, 1814) (Pisi Balığı)'un kromozom yapıları, sayıları ve sitogenetik özellikleri çeşitli bantlama yöntemleri kullanılarak incelenmiştir. İncelenen balık örnekleri Batı ve Orta Karadeniz sahili boyunca (Zonguldak ve Ordu illeri arası) 5 bölgeden avcılıkla elde edilmiştir. Büyük balıklara (250-1500 g) % 0,05 kolşisin solüsyonu (25-50 µg/g vücut ağırlığı) dorsalden kas arasına, ventralden karın boşluğuna enjekte edilmiştir. Küçük balıklar (50-250 g) ise % 0,005 kolşisin solüsyonunda 6 saat süre ile canlı bekletilmişlerdir. Diseksiyonla çıkarılan solungaç ve yüzgeç epitel dokuları % 0,4 KCl solüsyonunda 30 dk. bekletilip, Carnoy fiksatifi ile muamele edilmiştir. Kuruyan preparatlar en az 15 dakikalık süreyle giemsa (pH 6,8) solüsyonunda boyanmıştır

The first caryological description of European Flounder, Platichthys flesus luscus (Pallas, 1814) (Pisces, Pleuronectiformes) living in the Black Sea

In this study, the chromosome structures, numbers and other cytogenetic features of flounder, Platichthys flesus luscus (Pallas, 1814), which is one of the flatfish species in the Black Sea, were examined with using different banding methods. Fish samples were obtained from fishermen in 5 localities between Zonguldak and Ordu of the West and Middle Black Sea coasts. A total of 0.05 % of colchicine solution (25-50 µg / g body) weight was injected from intramuscular to dorsal and from intraperitonal to ventral for big fish (body weight 250 to 1500 g). However, small fish (body weight 50 to 250 g) were maintained in 0.005 % of colchicine solution for 6 hours. Gill and fin epithelia tissues derived by dissecting were incubated in 0.4 % of KCl solution for 30 mins and treated with Carnoy's fixative. Dried slides were stained with giemsa (pH 6.8) solution for 15 mins.

PDF

___

Arai, R., 2011. Fish Karyotypes A Check List. Springer Publication, Japan, 347p.
Barker, C.J., 1972. A method for the display of chromosomes of plaice, Pleuronectes platessa, and other marine fishes. Copeia, 2: 365-368. doi: 10.2307/1442503
Berendzen, P.B., Dimmick, W.W., 2002. Phylogenetic relationships of Pleuronectiformes based on molecular evidence. Copeia, 3: 642-652.
Stable URL: http://www.jstor.org/stable/1448146(10.02.2005) Berrebi, P., Agnese, J.F., Vianet, R., 1983. Esterases of flounder (Platichthys flesus, Pleuronectidae, Teleostei): Development of an identification protocol using starch gel electrophoresis and characterization of loci. Experientia, 46: 863-867. doi: 10.1007/BF01935540
Borsa, P., Quignard, J.-P., 2001. Systematics of the Atlantic-Mediterranean soles Pegusa impar, P. lascaris, Solea aegyptiaca, S. senegalensis, and S. solea and S. solea (Pleuronectiformes: Soleidae). Canadian Journal of Zoology, 79: 2297-2302. doi: 10.1139/z01-176
Brown, N.P., Bromage, N.R., Penman, D.J., Shields, R.J., 1997. The karyotype of the Atlantic halibut, Hippoglossus hippoglossus (Linneaus). Aquaculture Research, 28: 489-491. doi: 10.1046/j.1365-2109.1997.00871.x
Chapleau, F., 1993. Pleuronectiform relationships: a cladistic reassessment. Bulletin of Marine Science 52, 516-540.http://www. ingentaconnect.com/ content/umrsmas/ bullmar/1993/00000052/00000001/art00019#expand/collapse (24.04.2014)
Denton, T.E., 1973. Fish Chromosome Methodology, Charles C. Thomas Publisher, NewYork, 166 p.
Di, S., Knowles, J.F.,1994. The karyotype of the dab (Limanda limanda L.). Journalof Fish Biology, 44(2): 354-356. doi: 10.1111/j.1095-8649.1994.tb01213.x
Duran-Gonzalez, A., Garcia-Rucias, C.E., Laguarda-Figueras, A., 1990. The karyotype and G-bands of Haemulon aurolineatum Cuvier 1829 (Pisces: Haemulidae). Anales Del Insituto de Ciencias Del Mary Limnologia, 1-10. http://www.biblioweb.tic.unam.mx/cienciasdelmar/instituto/1990- 2/articulo373.html (06.08.2004)
Dutrillaux, B., Coutourier, J., 1981. La pratigue de l'analyse chromosomique techniques de laboratoire. No 12, Masson, Paris, 86 p. Exadactylos, A., Thorpe, J.P., 2001. Allozyme variation and genetic interrelationships between seven flatfish species (Pleuronectiformes).
Zoological Journal of the Linnean Society, 132: 487-499. doi: 10.1111/j.1096-3642.2001.tb02472.x Fan, Z., Fox, D.P., 1990. A new method for fish chromosome preparation. Journalof Fish Biology, 37: 553-561. doi: 10.1111/j.1095-8649.1990.tb05888.x
Fan, Z., Fox, D.P., 1991. Robertsonian polymorphism in plaice, Pleuronectes platessa L. and cod, Gadus morhua L. (Pisces, Pleuronectiformes and Gadiformes). Journal of Fish Biology, 38: 635-640. doi: 10.1111/j.1095-8649.1991.tb03152.x
Franke, U., Oliver, N., 1978. Quantitative analysis of high-resolution trypsinGiemsa bands on human prometaphase chromosomes. Human Genetics, 45: 137-165. doi: 10.1007/BF00286957
Froese, R., Pauly, D., 2015. Fishbase World Wide Web Electronic Publication, http://www.fishbase.org, version (01.03.2015)
Fukuoka, H., Niiyama, H., 1970. Notes on the somatic chromosomes of the species of pleuronectid fishes. Chromosome Information Service, 11: 18- 19.
Fujiwara,A., Fujiwara, M., Nishida-Umehara, C., Abe, S., Masaoka, T., 2007. Characterization of Japanese flounder karyotype by chromosomebandings and fluorescence in situ hybridization with DNAmarkers. Genetica, 131: 267-274. doi: 10.1007/s10709-006-9136-z
Fujiwara, A., Nishida-Umehara, C., Sakamoto, T., Okamoto, N., Nakayama, I., Abe, S., 2001. Improved fish lymphocyte culture for chromosome preparation. Genetica, 111: 77-89. doi: 10.1023/A:1013788626712
Galleguillos, R.A., Ward, R.D., 1982. Genetic and morphological divergence between populations of the flatfish Platichthys flesus (L.) (Pleuronectidae). Biological Journal of the Linnean Society, 17: 395-408. doi: 10.1111/j.1095-8312.1982.tb02029.x
Gold, J.R., Li, Y.C., Shipley, N.S., Powers, P.K., 1990. Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. Journal of Fish Biology, 37: 563-575. doi: 10.1111/j.1095-8649.1990.tb05889.x
Jankun, M., Ocalewicz, K., Pardo, B.G., Martinez, P., Woznicki, P., Sanchez, L., 2003. Chromosomal characteristics of rDNA in European grayling Thymallus thymallus (Salmonidae). Genetica, 119: 219-224. doi: 10.1023/A:1026022415908
Kikuno, T., Ojima, Y., Yamashita, N.,1986. Chromosomes of flounder, Paralichthys olivaceus. Proceedings of the Japan Academy, 62: 194- 196. doi: 10.2183/pjab.62.194
Kim, D.S., Cheong, S.C., Park, S.R., Lee, J.K., 1988. Cytogenetic and biochemical studies on the flatfish, Paralichthys olivaceus. Bulletin of National Fisheries Research and Development Agency, 42:135-142.
Kligerman, A.D., Bloom, S.E., 1977. Rapid chromosome preparations from solid tissues of fishes. Journal of Fisheries Research Board of Canada, 34: 266-269. doi: 10.1139/ f77-039.
Klinkhardt, M.B., 1993.Zur zytogenetik von Platichthys flesus und Limanda limanda (Pleuronectidae, Teleostei) und karyoevolution der Pleuronectiformes. Zeitschrift für Fischkunde, 2: 65-75.
Klinkhardt, M.B., Tesche, M., Greven, H., 1995. Databas e of Fish Chromosomes, Westarp Wissenchaften Verlag Wolf Graf von Westarp, Uhlichstraße 6, 39108 Magdeburg, Germany, 237 p.
Levan, A., Fredga, K., Sandberg, A.A., 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220. doi: 10.1111/j.1601-5223.1964.tb01953.x
Macgregor, H.C., Varley, J.M., 1983. Chapter 4. Chromosome banding.In:Working with animal chromosomes.John Wiley and Son Ltd., Chichester, New York, Brisbane, Toronto, Singapore. p 70-96.
Nelson, J.S., 2006. Fishes of the World, IV. Edition. John Wiley and Sons Inc., New York, USA, 622 p. Nogusa, S., 1960. A comparative study of the chromosomes in fishes with particular considerations on taxonomy and evolution. Memoirs of the Hyogo University of Agriculture, 3: 1-62.
Ohno, S., Atkin, N.B., 1966. Comparative DNA values and chromosome complements of eight species of fishes. Chromosoma, 18: 455-466. doi: 10.1007/BF00332549
Pardo, B.G., Bouza, C., Castro, J., Martinez, P., Sanchez, L., 2001. Localization of ribosomal genes in Pleuronectiformes using Ag-, CMA3- banding and in situ hybridization. Heredity 86, 531-536. doi: 10.1046/j.1365-2540.2001.00802.x
Park, I.S., Nam, Y.K., Douglas, S.E., Johnson, S.C., Kim, D.S., 2003. Genetic characterization, morphometric and gonad development of induced interspecific hybrids between yellowtail flounder, Pleuronectes ferrugineus (Storer) and winter flounder, Pleuronectes americanus (Walbaum). Aquaculture Research, 34: 389-396. doi: 10.1046/j.1365-2109.2003.00816.x
Reeves, A., 2001. MicroMeasure: A new computer program for the collection and analysis of cytogenetic data. Genome, 44: 439-443. doi: 10.1139/g01-037
Rossi, A.R., Crosetti, D., Gornung, E., Sola, L., 1996. Cytogenetic analysis of global populations of Mugil cephalus (Striped mullet) by different staining techniques and fluorescent in situ hybridization. Heredity, 76: 77-82. doi: 10.1038/hdy.1996.10
Sakamoto, K., Nishikawa, S., 1980. Chromosomes of three flatfishes (Pleuronectiformes). Japanese Journal of Ichthyology, 27:268-272. doi: 10.11369/jji1950.27.268
Seabright, M., 1971. Rapid banding techniques for human chromosomes. The Lancet, 2: 971-972. doi: 10.1016/S0140-6736(71)90287-X
Thorgaard, G.H., Disney, J.E., 1990. Chapter 6. Chromosome Preparation and Analysis. In:Methods for Fish Biology:, Edit. by Schreck, C. B. Moyle, P. B. American Fisheries Society, Bethesda, Maryland, USA, pp. 171-190.
Welcher, F., 1966. Chemical solutions. D.Van Nostrand Company Ltd., Canada, 404 p. Yabu, H., Ishii, K.,1980. Chromosomes of flatfish Glyptocephalus stelleri (Schmidt). Chromsome Information Service, 29 :16.