T. Jawahar ABRAHAM, Suman MONDAL, Ch Surendra BABU

Effect of commercial aquaculture probiotic and fish gut antagonistic bacterial flora on the growth and disease resistance of ornamental fishes Carrassius auratus and Xiphophorus helleri

Akuakültür probiyotiği ve mide antogonistik bakteriyel florasının Carassius auratus ve Xiphophorus helleri’de gelişim ve hastalık direncine etkisi. Bu çalışmada ticari bir akuakültür biyotiğinin (CA probiyotik) ile Lactobacillus sp. ve Bacillus sp. (FG probiyotik) gibi balık midesinde bulunan antogonistik bakteriyel floranın bir karışımının süs balıklarından Carassius auratus ve Xiphophorus helleri türlerinin gelişimi ve hastalık direnci üzerine etkisi araştırılmıştır. 5g/kg seviyesinde CA probiyotik ile beslenen C. auratus’ta gelişiminde olumlu bir etkisi olmadığı ve aksine kontrol grubu ile karşılaştırıldığında büyümede yavaşlama gözlenmiştir. Benzer şekilde X. helleri’de de toplam yaş ağırlık kazanımı, besin tüketim oranı (FCR) ve spesifik büyüme oranı (SGR) üzerinde önemli bir etkisi bulunamamıştır. Bir başka açıdan, FG probiyotik içerikli besinler ile beslenen C. auratus bireylerinde FCR dışında (p0.05). Ancak FG probiyotik ilaveli besleme, kontrol grubu ile karşılaştırıldığında yüzgeç ve kuyrukta oluşan çürümelerde önemli farklılıklar göstermiştir.

Anahtar Kelimeler:

hastalığa dayanıklılık, Bacillus, Lactobacillus, probiyotikler, mikrobiyal flora, rumen florası, japon balığı

Akuakültür probiyotiği ve mide antogonistik bakteriyel florasının Carassius auratus ve Xiphophorus helleri'de gelişim hastalık direncine etkisi

Effect of commercial aquaculture probiotic (CA probiotic) and a mixture of fish gut antagonistic bacterial flora such as Lactobacillus sp. and Bacillus sp. (FG probiotic) on the growth and disease resistance of ornamental fishes Carassius auratus and Xiphophorus helleri was investigated. The CA probiotic at a level of 5g / kg feed had no effect on the growth rate of C. auratus, rather it reduced the growth compared to control group. Also it had no significant effect on the total wet weight gain, food conversion ratio (FCR) and specific growth rate (SGR) of X. helleri. On the other hand, in FG probiotic feed fed C. auratus, except FCR (P<0.04), the variations in growth parameters were statistically insignificant. While in X. helleri, there existed significant differences in the total wet weight gain and FCR of FG probiotic feed fed and control groups. The CA and FG probiotic feed fed fishes did not show any significant protection (P>0.05) against Pseudomonas fluorescens 58C infection. However, marked differences in the fishes exhibiting fin and tail rot were noticed between the FG probiotic fed and control groups

Keywords:

disease resistance, Bacillus, Lactobacillus, probiotics, microbial flora, rumen flora, goldfish,

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Abraham, T. J., CH. S. Babu, and T. Banerjee. 2007a. Influence of a fish gut bacterium Lactobacillus sp. on the production of swordtail Xiphophorus helleri (Heckel 1848). Bangladesh J. Fish. Res., 11(1): 65-74
Abraham, T. J., CH. S. Babu, S. Mondal, and T. Banerjee. 2007b. Effects of dietary supplementation of commercial human probiotic and antibiotic on the growth rate and content of intestinal microflora in ornamental fishes. Bangladesh J. Fish. Res., 11(1): 57-63
Abraham, T. J. 2008. Antagonistic fish gut bacterium Lactobacillus sp. as biocontrol agent in ornamental fish Culture. Fish Technol., 45 (2): (In Press)
Aly, S M., Y. A. Ahmed, A. A. Ghareeb, and M. F. Mohamed. 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish Shellfish Immunol., 25, 128 – 136
Austin, B., L. F. Stuckey, P. A. W., Robertson, I. Effendi, and D. R. W. Griffith. 1995. A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. J. Fish Dis. 18: 93-96.
Balca´zar, J L., T. Rojas-Luna. 2007. Inhibitory activity of probiotic Bacillus subtilis UTM 126 against Vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei). Curr. Microbiol., 55:409–412
Bogut, I., Z. Milakovic, S. Brkic, and R. Zimmer. 1998. Influence of probiotic (Streptococcus faecium M74) on growth and content of intestinal microflora in carp (Cyprinus carpio). Czech J. Anim. Sci. 43: 231-235.
Carnevali, O., M. C. Zamponi, R. Sulpizio, A. Rollo, M. Nardi, C. Orpianesi, S. Silvi, M. Caggiono, A. M. Polzonetti, and A. Cresci. 2004. Administration of probiotic strain to improve sea bream wellness during development. Aquaculture Int., 12: 377–386.
Epifario, C.E. 1979. Comparison of yeast and algal diets for bivalve molluscs. Aquaculture 16: 187-192.
Gatesoupe, F.J. 1991. Bacillus sp A new tool against bacterial infection in turbot larvae, Scophthalmus maximus. Aquat. Living Res. 10: 239-246. Gatesoupe, F.J. 1994. Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmus maximus, against pathogenic Vibrio. Aquat. Living Res. 7: 277-282.
Irianto, A., B. Austin. 2002. Probiotics in aquaculture. J. Fish Dis. 25: 633-642.
Kozasa, M. 1986. Toyocerin (Bacillus toyoi) as growth promoter for animal feeding. Microbiologia Aliments Nutrition 4: 121-125.
Lara-Flores, M., M. A. Olvera-Novoa, B. E. Guzman-Mendez, and W. Lopez-Madrid. 2003. Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture, 216: 193–201.
Maeda, M., I. C. Liao. 1992. Effect of bacterial population on growth of a prawn larva, Penaeus monodon. Bull. Nat. Res. Inst. Aquacult. 21: 25-29.
Mohanty, S.N., S. K. Swain, and S. D. Tripathi. 1996. Rearing of catla (Catla catla Ham.) spawn on formulated diets. J. Aqua. Trop. 11: 253-258.
Murthy, H.S. and A.T.R Naik. 2002. Growth performance of Cirrhinus mrigala in relation to a natural probiotic feed supplement, Biovet-YC. Fish. Chimes, 22: 54-56.
Robertson, P.A.W., C. O. Dowd, C. Burrells, P. Williams and B. Austin. 2000. Use of Carnobacterium sp as a probiotic for Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss, Walbaum). Aquaculture, 185: 235-243
Sakata, T., 1990. Microflora in the digestive tract of fish and shellfish, p. 171-176. In R. Lesel (ed.), Microbiology in Poecilotherms. Elsevier, Amsterdam
Salinas, I., A. Cuesta, M.A. Esteban, and J. Meseguer. 2005. Dietary administration of Lactobacillus delbrueckii and Bacillus subtilis, single or combined, on gilthead seabream cellular innate immune responses. Fish Shellfish Immunol., 19: 67-77.
Snedecor, G.W., W. G. Cochran. 1974. Statistical Methods, 7th printing, 6th edition. Iwa State University Press, Ames, Iwa, U.S.A., p593
Uma, A., T. J. Abraham, M. J. P. Jeyaseelan, and V. Sundararaj. 1999. Effect of probiotic feed supplement on performance and disease resistance of Indian white shrimp Penaeus indicus H. Miline Edwards. J. Aqua. Trop. 14: 159-164.
Vaseeharan, B., P. Ramasamy. 2003. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett. Appl. Microbiol., 36: 83–87