İzmir ilinde çeşitli su kaynaklarındaki Caulobacter'lerin karakterizasyonu ve antibiyotik dirençlerinin incelenmesi
Caulobacter, uzantılı bakteriler grubunun farklı bir genusudur. Bu bakteriler, hayat döngüleri boyunca tek flajelli yüzücü hücre ve bir sapa sahip hareketsiz hücre formlarını sırayla oluşturmaktadır. Denizden ve kıyı bölgelerinden olduğu gibi topraktan ve tatlısulardan izole edilebilirler. Bu çalışmada, İzmir ilindeki 10 farklı su kaynağından alınan örneklerden 29 Caulobacter genusu üyesi izole edilmiştir. Yapılan mikroskobik incelemeler ve biyokimyasal testler sonucunda, 9 izolatın C. leidyi, 14’ünün C. variabilis, 6’sının C. intermedius olduğu belirlenmiş ve 7 farklı antibiyotiğin bu bakterilerin büyümesi üzerine etkisi tespit edilmiştir.
Characterizations of Caulobacters in different water sources in İzmir and investigation of antibiotic resistance of them
Characterizations of Caulobacters in different water sources in İzmir and investigation of antibiotic resistance of them. Caulobacter is a distinctive genus of prosthecate bacteria. During their life cycle these bacteria alternate between monoflagellated, swarmer cell and a nonmotile cell which has a stalk. They can be isolated from soil and freshwater as well as marine and estuarine locations. In this research, 29 members of Caulobacter genus from 10 different water sources which are located in Izmir region were isolated. At the end of the microscobic investigations and biocemical analyses, its observed that 9 strains belong to species C. leidyi, 14 strains belong to species C. variabilis, 6 strains belong to species C. intermedius and effects of the 7 different antibiotic on the growth of these bacteria were researched.
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- Anast, J., J. Smit. 1988. Isolation and Characterization of Marine Caulobacter and Assesment of Their Potential for Genetic Experimentation. Appl. Environ. Microb., 54:809-817.
- Awram, P., J. Smit. 2001. Identification of Lipopolisaccaride O Antigen Synthesis Genes Required for Attachment of the S-Layer of Caulobacter crescentus. Microbiology., 147:1451-1460.
- Bradhshow, L. J. 1963. Laboratory Microbiology. WB Saunders Company. p. 289. Philadelphia and London.
- Ely, B. 1979. Transfer of Drug Resistance Factors to the Dimorphic Bacterium Caulobacter crescentus. Genetics., 91: 371-380.
- Jacob, L. S. 1992. Farmacology, (in Turkish), (çev. S. Koşay), Saray Tıp Kitabevi. 366 s. İstanbul.
- Lafferty, R. M., B. Korsatko, and W. Korsatko. 1988. Production of Poly-β-hydroxybutryic acid. Biotechnol., Vol: 6b, 135-176.
- Laub, M. T., H. H. McAdams, T. Feldbium, C. M. Fraser and L. Shapira. 2000. Global Analysis of the Genetic Network Controlling the Caulobacter Cell Cycle. Science., 290: 2144-2148.
- MacRae, J. D., J. Smit. 1991. Characterization of Caulobacters Isolated from Waste Water Treatment Systems. Appl. Environ. Microb., 57: 751-758.
- Madigan, M. T., J. M. Martinko and J. Parker. 2003. Brock Biology of Microorganisims (10th Edition). p. 1019. New Jersey.
- Njaka-Umelo, E., J. F. Nomellini, W. H. Bingle, L. G. M. Glasier, R. T. Irvin and J. Smit. 2001. Expression and Testing of Pseudomonas aeroginosa Vaccine Candidate Proteins Prepared with the Caulobacter crescentus S-Layer Protein Expression System. Vaccine., 19: 1406-1415.
- Nomellini, J. F., S. Kupcu, U. B. Sleytr and J. Smit. 1997. Factors Controlling in vitro Recrystallization of the Caulobacter crescentus Paracrystalline S-Layer. J. Bacteriol., 179: 6349-6354.
- Poindexter, J. S. 1964. Biological Properties and Classification of the Caulobacter Group. Bacteriol. Rev., Vol: 28, 231-395.
- Poindexter, J. S. 1981. The Caulobacters: Ubiquitous Unusual Bacteria. Microbiol. Rev., 45: 123-179.
- Poindexter, J. S. 1986. Bacteria that Divide by Binary Transverse Fission. In: Bergey’s Manuel of Systematic Bacteriology (eds. J. T. Staley, M. P. Bryant, N. Pfenning, J. G. Holt) pp: 1924-1939. Vol 3, Williams&Wilkins, Baltimore.
- Poindexter, J. S. 1991. Dimorphic Prosthecate Bacteria. In: The Prokaryotes (eds. A. Ballows, H. G. Truper, M. Dwarkin, W. Harder, K. H. Schleifer) pp: 2176- 2196. Vol: 2, New York.
- Reichelt, M., B. U. Von Specht and H. P. Hahn. 2001. The Caulobacter crescentus Outer Membrane Proteine Omp58 (RsaA) is not Required for Paracrystalline S-Layer Secretion. FEMS Microbiol. Lett., 201: 277-283.
- Stahl, D. A., R. Key, B. Flesher and J. Smit. 1992. The Phylogeny of Marine and Fresh Water Caulobacters Reflects Their Habitat. J. Bacteriol., 2193-2198.
- Wingrove, J. A., J. W. Gober. 1995. Caulobacter crescentus Overview and Cell Cycle. Dev. Biol., 6: 325-333.