İsmail KARABOZ, ATAKAN SUKATAR, Aslı PARLAKAY

Denizsel organizmalardan elde edilen yeşil florensans protein (GFP) ve kullanım alanları

Çoğu denizsel organizma lüminesens özelliklere sahiptir. Işık oluşturan proteinler, primer ışık üreticiler (aequorin ya da lusiferaz) ve denizde daha iyi nüfuz etmesi için kırmızı ışığı değiştiren sekonder fotoproteinlerdir. Bir sekonder protein olan Yeşil floresans protein (GFP) floresans özellikte olması, modifiye olmuş amino asit kalıntılarından oluşan floroforu bulunması ve üç boyutlu yapısı nedeniyle sıra dışı özelliklere sahiptir. Deniz anası Aequorea victoria ve deniz menekşesi Renilla reniformis türlerinden izole edilen GFP, bu canlılarda enerji transferinde rol oynamaktadır. Bu çalışmada, denizsel organizmalardan elde edilen GFP’nin moleküler yapısı açıklanmış ve günümüzdeki kullanım alanlarına değinilmiştir.

Anahtar Kelimeler:

proteinler, sucul kültür, lusiferaz

Green fluorescent protein (GFP) isolated from marine organisms and its usages

Many marine organisms are luminescent. The proteins that produce the light are primary light producers (aequorin or luciferase) and secondary proteins that shifts the red light for better penetration into the ocean. Green fluorescent protein (GFP) which is a secondary protein has extraordinary characteristics because of the fact that it is fluoroscent, has fluorophore made up of modified amino acid residues and has 3D structure. GFP isolated from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis functions as energy-transfer acceptors in these organisms. In this study, the molecular structure of GFP that is isolated from marine organisms was explained and its recently usage was discussed.

Keywords:

proteins, aquaculture, luciferase,

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