ŞÜKRAN ÇAKLI, Aslı YÜNLÜ CADUN, M.Tolga DİNÇER, Serap DOĞAN, CENGİZ KOÇAK

Comparison of polyphenol oxidase activity in different parts of deep water pink shrimp (Parapenaeus longirostris) by using L-DOPA substrate in Marmara Sea during the fishing season

Melanosis derin su pembe karidesinin pazar kaybı için önemli bir problemdir. Bunun sorumlusu olan polifenol oksidaz (PPO) enzim aktivitesidir. PPO’nun özelliğini daha iyi anlamak ve oluşan aktiviteyi düşürme amacı için, ölüm sonrası derin su pembe karidesi türünün(Parapenaus longristris) farklı dokularında polifenol oksidaz (PPO) enzim aktivitelerinin tespiti çalışılmıştır. Aktivite analizleri yedi ayrı dokuda çalışılmıştır: kafa (seflahalotoraks+karapas), karapas, dış iskeletin abdomen bölgesi, yüzme bacakları, yürüme bacakları, uropodlar ve telson bölgelerinde ayrı ayrı analizleri yapılmıştır. Sonuçlar doğrultusunda derin su pembe karidesinde PPO aktivite dağılımının kafadan telsona doğru azaldığı tüm çalışma boyunca tespit edilmiştir. Ayrı dokularda yapılan dağılım incelemesinde %70 amonium sulfat çökeltmesi sonucunda en yüksek aktivite kafa ve abdomen arasında kalan bölgede görülmüştür. Fakat cinsiyetler arasında istatistiksel anlamda önemli bir fark görülmemiştir. Ayrıca PPO aktivite düzeylerinin kıyaslanması tüm av sezonu boyunca yapılmıştır. Türün önemi bu türün ekonomik değerinden gelmektedir ıve literatürde Marmara Denizinden avlanan türle ilgili PPO aktivitesi ile ilgili kaynağa rastlanmamıştır. Elde edilen veriler Marmara Ddenizinde bulunan erkek ve dişi karideslerin mevsimsel aktivite düzeylerinide göstermektedir.

Marmara Denizinde av sezonu boyunca derin su pembe karidesi (Parapenaeus longirostris) farklı dokularındaki polyfenol oksidaz enzim aktivitesinin L-Dopa substratı kullanılarak karşılaştırılması

Melanosis is a major problem associated with the marketing loss of deep water pink shrimp. This is mediated by polyphenoloxidase (PPO), activity. To better understanding of PPO characteristics should pave a way for lowering melanosis so tissue distribution of polyphenol oxidase (PPO) activity was studied in deep water pink shrimp (Parapenaus longristris) post mortem. Activity analyzed in 7 different tissues; the head (cephalothorax+carapace), carapace, exoskeleton of the abdomen, pereopods, pleopods, uropods and telson individually. Due to the results, PPO activity of deep water pink shrimp was determined to be lower from head to telson during the research period. Partial tissue distribution showed that the highest activity was determined in the parts between head and the abdomen by using 70% ammonium sulfate fractions. But statistical significant correlation cannot be detected between the genders. And also comparison between the PPO levels of deep water pink shrimp during the fishing season was performed. The importance of these species came from their economic values, and no available data about the PPO activity in the literature could be observed in Marmara Sea. The determined data showed us the seasonal activity levels of male and female shrimps in Marmara Sea.

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