MERT GÜRKAN, Seda SERBEST, SİBEL HAYRETDAĞ

Acute toxicity of the synthetic pyrethroid alpha-cypermethrin on the tadpoles of variable green toad, Bufotes variabilis (Amphibia:Anura)

Bu çalışmada, Bufotes variabilis (Pallas, 1769) iribaşları üzerinde alfa-sipermetrinin akut toksik etkileri araştırıldı. Gosner 19. evredeki iribaşlar, 96 saat süreyle 0; 0,1; 5 ve 10 µgl-1 konsantrasyonlarda alfa-sipermetrine maruz bırakıldı. Çalışma sonunda; mortalite, bazı morfolojik ölçümlerdeki değişiklikler (burun-anüs açıklığı arası uzunluğu, vücut genişliği, kuyruk uzunluğu ve ıslak ağırlık), morfolojik anomaliler ve davranışsal değişiklikler değerlendirildi. 96 saatlik LC50 değeri 15,62 µgl-1 olarak hesaplandı. 5 ve 10 µgl-1 alfa-sipermetrin uygulaması yapılan iribaşların, %83 ve %95'inde aksiyal anomali, viseral ödem, ağız deformasyonu ve kuyruk deformasyonu gibi bazı morfolojik değişiklikler gözlendi. Alfa-sipermetrin uygulaması yapılan tüm iribaşlarda yüzme mesafesinde kısalma ve dengesizlik gibi davranışsal değişiklikler kaydedildi. Tüm bulgular birlikte değerlendirildiğinde, alfa-sipermetrinin, B. variabilis iribaşlarının gelişimi üzerine olumsuz etkileri olduğu sonucuna ulaşıldı.

Sentetik piretroit alfa-sipermetrinin değişken desenli gece kurbağası, Bufotes variabilis iribaşlarındaki akut toksisitesi (Amphibia: Anura)

In this study, acute toxic effects of alpha-cypermethrin were investigated in the tadpoles of Bufotes variabilis (Pallas, 1769). Tadpoles at Gosner stage 19 were exposed to 0, 0.1, 5 and 10 µgl-1 of alpha-cypermethrin concentrations for 96 hours. Mortality, the changes in some morphological measurements (snoutvent length, body width, tail length, and wet weight), morphological anomalies and behavioral changes were evaluated at the end of the experiment. The 96 hours LC50 value was calculated as 15.62 µgl-1. Such morphological changes as axial anomaly, visceral edema, deformation of the mouth, and tail deformation were observed in 83% and 95% of the tadpoles in the groups treated with 5 and 10 µgl-1 of alpha-cypermethrin. Behavioral changes such as shortening of the swimming distance and immobility were recorded in all tadpoles exposed to alpha-cypermethrin. When all findings were evaluated totally, it was concluded that alphacypermethrin had negative effects on the development of the tadpoles of B. variabilis.

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