Emre Cem ERASLAN, Deniz ALTUNTAS, Hayri BABA, Celal BAL, Hasan AKGÜL, Ilgaz AKATA, Mustafa SEVİNDİK

Some biological activities and element contents of ethanol extract of wild edible mushroom morchella esculenta

In this study, antioxidant, oxidant, antimicrobial activity and heavy metal contents of Morchella esculenta (L.) Pers. mushroom collected from Konya (Turkey) province were determined. Antioxidant and oxidant values were determined by using Rel Assay TAS and TOS kits. Element contents were determined by AAS (atomic absorption spectroscopy). Antimicrobial activities were determined by agar dilution method against bacteria and fungus strains. As a result of the study, TAS (total antioxidant status) value of mushroom was determined as 4.580±0.114 mmoL/L, TOS (total oxidant status) value was determined as 13.549±0.211 μmoL/L and OSI (oxidative stress index) value was found to be 0.296±0.003. Mushroom extracts were found to be effective at concentrations 50-200 μg/mL against the test microorganisms. In addition, the contents of Fe, Cu, Zn, Pb, Ni, Mn, Co, Cd and Cr were determined as 264.57±17.27, 14.77±0.14, 7.82±0.34, 12.59±0.89, 0.53±0.08, 19.45±0.97, 3.82±0.13, 2.45±0.02 and 6.86±0.08 mg.kg-1, respectively. It was determined that M. esculenta can be one of the antioxidant and antimicrobial sources which can be taken by diet.

Keywords:

Antioxidant, antimicrobial, edible mushroom, heavy metal, Morchella esculenta,

PDF

___

[1] Kumar Raman V., Saini M., Sharma A., Parashar D. B., Probiotic properties of lactic acid bacteria isolated from animal sources. International Journal of Development Research 2018;8(3):19660-19665.
[2] Larson A. J., Cansler C. A., Cowdery S. G., Hiebert S., Furniss T. J., Swanson M. E., Lutz JA. Post-fire morel (Morchella) mushroom abundance, spatial structure, and harvest sustainability. Forest Ecology and Management 2016;377:16-25.
[3] O’Donnell K., Rooney A. P., Mills G. L., Kuo M., Weber N. S., Rehner S. A., Phylogeny and historical biogeography of true morels (Morchella) reveals an early Cretaceous origin and high continental endemism and provincialism in the Holarctic. Fungal Genetics and Biology 2011; 48(3):252-265.
[4] Cui H. L., Chen Y., Wang S. S., Kai G. Q., Fang Y. M., Isolation, partial characterisation and immunomodulatory activities of polysaccharide from Morchella esculenta. Journal of the Science of Food and Agriculture 2011;91(12):2180-2185.
[5] Heleno S. A., Stojković D., Barros L., Glamočlija J., Soković M., Martins A., Queiroz MJRP, Ferreira IC. A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. Food Research International 2013;51(1):236-243.
[6] Li S., Gao A., Dong S., Chen Y., Sun S., Lei Z., Zhang Z., Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta. International journal of biological macromolecules 2017;96:26-34.
[7] Liu C., Sun Y., Mao Q., Guo X., Li P., Liu Y., Xu N., Characteristics and antitumor activity of Morchella esculenta polysaccharide extracted by pulsed electric field. International Journal of Molecular Sciences 2016;17(6):986.
[8] Liu Q., Ma H., Zhang Y., Dong C., Artificial cultivation of true morels: current state, issues and perspectives. Critical Reviews in Biotechnology 2018;38(2):259-271.
[9] Nitha B., Fijesh P. V., Janardhanan K. K., Hepatoprotective activity of cultured mycelium of Morel mushroom, Morchella esculenta. Experimental and Toxicologic Pathology 2013;65(1-2): 105-112.
[10] Nitha B, Meera CR, Janardhanan KK. Anti-inflammatory and antitumour activities of cultured mycelium of morel mushroom, Morchella esculenta. Current Science 2007;92(2):235-239.
[11] Wagay J.A., Nayik G.A., Wani S.A., Mir R.A., Ahmad M.A., Rahman Q.I., Vyas D., Phenolic profiling and antioxidant capacity of Morchella esculenta L. by chemical and electrochemical methods at multiwall carbon nanotube paste electrode. Journal of Food Measurement and Characterization 2019;1-15.
[12] Erel O. A., Novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 2004;37(4):277-285.
[13] Erel O. A., New automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 2005;38(12):1103-1111.
[14] Hindler J., Hochstein L., Howell A., (1992) Preparation of routine media and reagents used in antimicrobial susceptibility testing. Part 1. McFarland standards, In H. D. Isenberg (ed) Clinical microbiology procedures handbook. American Society for Microbiology 1992;1:5.19.1-5.19.6. Washington, D.C.
[15] CLSI (Clinical and Laboratory Standards Instıtute). (2002) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard-Second Edition 2002. NCCLS document M27-A2 (ISBN 1-56238-469-4), NCCLS Pennsylvania USA.
[16] CLSI (Clinical and Laboratory Standards Instıtute). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically 2003. Approved Standard-Seventh Edition. CLSI Document M7-A7, Pennsylvania USA.
[17] Sevindik M., Antioxidant activity of ethanol extract of Daedaleopsis nitida medicinal mushroom from Turkey. Mycopath 2018;16(2):47-49.
[18] Athanasakis G, Aligiannis N, Gonou-Zagou Z, Skaltsounis AL, Fokialakis N. Antioxidant properties of the wild edible mushroom Lactarius salmonicolor. Journal of Medicinal Food 2013;16(8):760-764.
[19] Yildiz S., Yılmaz A., Can Z., In vitro bioactive properties of some wild mushrooms collected from Kastamonu province. Kastamonu Üniversitesi Orman Fakültesi Dergisi 2017;17(3):523-530.
[20] Sevindik M., Akgul H., Bal C., Selamoglu Z., Phenolic contents, oxidant/antioxidant potential and heavy metal levels in Cyclocybe cylindracea. Indian Journal of Pharmaceutical Education and Research 2018;52(3):437-441.
[21] Sevindik M., Akgul H., Selamoglu Z., Braidy N., Antioxidant and Antigenotoxic Potential of Infundibulicybe geotropa Mushroom Collected from Northwestern Turkey. Oxidative Medicine and Cellular Longevity 2020;2020:5620484.
[22] Sevindik M., Akata I., Antioxidant, oxidant potentials and element content of edible wild mushroom Helvella leucopus. Indian Journal of Natural Products and Resources 2019;10(4):266-271.
[23] Sevindik M., The novel biological tests on various extracts of Cerioporus varius. Fresenius Environmental Bulletin 2019;28(5):3713-3717.
[24] Sevindik M., Investigation of antioxidant/oxidant status and antimicrobial activities of Lentinus tigrinus. Advances in Pharmacological Sciences 2018.
[25] Avcı E., Cagatay G., Avcı G. A., Cevher S. C., Suicmez M., An edible mushroom with medicinal significance; Auricularia polytricha. Hittite Journal of Science & Engineering 2016;3(2):111-116.
[26] Mau J. L., Chang C. N., Huang S. J., Chen C. C., Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Food chemistry 2004;87(1):111-118.
[27] Badshah H., Qureshi R. A., Khan J., Ullah F., Fahad S., Ullah F., Khan A. M., Hussain I., Khan N., (2012) Pharmacological screening of Morchella esculenta (L.) Pers., Calvatia gigantea (Batsch ex Pers.) Lloyd and Astraeus hygrometricus Pers., mushroom collected from South Waziristan (FATA.). Journal of Medicinal Plants Research 2012;6(10):1853-1859.
[28] Zhang Q., Wu C., Fan G., Li T., Wen X., Characteristics and enhanced antioxidant activity of glycated Morchella esculenta protein isolate. Food Science and Technology 2018;38(1):126-133.
[29] Altuner E. M., Akata I., Antimicrobial Activity of Some Macrofungi Extracts. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2010;14(1):45-49
[30] Sevindik M., Rasul A., Hussain G., Anwar H., Zahoor M. K., Sarfraz I., Kamran K.S., Akgul H., Akata I., Selamoglu Z., (2018) Determination of anti-oxidative, anti-microbial activity and heavy metal contents of Leucoagaricus leucothites. Pakistan Journal of Pharmaceutical Sciences 2018;31(5): 2163-2168.
[31] Sevindik M., Akgul H., Akata I., Alli H., Selamoglu Z., Fomitopsis pinicola in healthful dietary approach and their therapeutic potentials. Acta alimentaria 2017;46(4):464-469.
[32] Svoboda L., Chrastný V., Levels of eight trace elements in edible mushrooms from a rural area. Food Additives and Contaminants 2008;25(1):51-58.
[33] Zhu F., Qu L., Fan W., Qiao M., Hao H., Wang X., Assessment of heavy metals in some wild edible mushrooms collected from Yunnan Province, China. Environmental monitoring and assessment 2011;179(1):191-199.
[34] Sevindik M., Akgul H., Dogan M., Akata I., Selamoglu Z., (2018) Determination of antioxidant, antimicrobial, DNA protective activity and heavy metals content of Laetiporus sulphureus. Fresenius Environmental Bulletin 2018;27(3):1946-1952.

Sigma Mühendislik ve Fen Bilimleri Dergisi-Cover

ISSN: 1304-7191
Yayın Aralığı: Yılda 4 Sayı
Yayıncı: Yıldız Teknik Üniversitesi

Arşiv