The continuous importation of fish portends a colossal loss of foreign exchange reserved to Nigeria which requires urgent attention to boost fish production. It is against this backdrop that this study analyzed the profitability analysis of catfish production in Edo State, Nigeria. It specifically describe the production characteristics of the catfish farmers, estimate the input and output quantities of catfish, determine the profitability and identify the constraints associated with catfish production in the study area. Multi-stage sampling procedure was employed to select a total of 468 catfish farmers from the study area. Data collection was achieved through the administration of structured questionnaire. Data analysis was done using descriptive statistics, budgetary techniques and 4- Point Likert-type scale. The results revealed that the farmers in the study area used more of personal savings (58.12%), family land (59.40%) and local feed (82.48%). Stocked more at juvenile stage (70.30%) and produced average output of 4859.51kg per production cycle. The results also showed the average total cost incurred and revenue realized were N 543.67 and N 752.56 per kg fish respectively. The catfish production was profitable with average gross margin, net profit and return per naira invested of N224.35, N208.90 and N1.38 per production cycle respectively. The major constraints faced by catfish farmers were high cost of food (3.96), lack of capital (3.65) and lack of inadequate power supply (3.51) are very serious constraints among others. Since the catfish production was profitable, the farmers should be encouraged to combat these constraints and expand their holdings to boost production.