Sugarcane is a plant whose quality parameters are required to be determined both for being one of the substances used in sugar production and for being used as animal feed. Near-infrared spectroscopy is a technique that has already been used for predicting the parameters of various plants and has gained popularity in recent years. This study proposes a near-infrared spectroscopy-based model for the rapid and effortless analysis of acid detergent fiber fraction and vitro organic matter digestibility parameters of the sugarcane plant. Partial least squares regression was combined with common preprocessing methods for modeling. This model yielded an R2 CV value of 0.935 and 0.953 for the acid detergent fiber fraction and vitro organic matter digestibility parameters, respectively. Then, the spectra from three handheld spectrometers were combined using a proposed combination method to generate new spectra with higher spectral resolution. New models were built using these generated spectra and compared to the previous result. Obtained results showed that combining spectra from different spectrometers can improve model performance.