The aim of the present study was to conduct preliminary phytochemical screening, pharmacognostical and physicochemical investigation of Chrysochamela noeana. The fresh and dried herbs were studied by morphology, microscopy, preliminary phytochemical screening and florescence analysis of powdered drug. In addition, physicochemical parameters were studied according to WHO guidelines. Physicochemical parameters and florescence analysis were also performed. The preliminary phytochemical analysis revealed the presence of steroids, flavonoids, phenolic compounds, and carbohydrates. Total phenol contents were 245 ± 3.67, 179.06 ± 4.52, and 15.98 ± 3.32 (GAE mg/g extract) and total flavonoid content were 180.85 ± 8.21, 146.41 ± 2.56, and 13.46 ± 0.23 (QUE mg/g extract) for ethyl acetate, methanol, and water extracts, respectively. The IC50 value for extracts in ABTS radical scavenging activity were calculated in order of 51.47, 122.26 and 1146.91 µg/mL, and in DPPH for 1.55, 1.13 and 27.16 mg/mL for ethyl acetate, methanol, and water extracts, respectively. The results of these studies could be useful for correct identification and detection of adulterants from this plant material.