Farklı adeziv uygulama tekniklerinin dentine mikrogerilim bağlanma dayanımlarının değerlendirilmesi

Amaç: Bu çalışmanın amacı, basitleştirilmiş adeziv sistemlerde farklı adeziv uygulama tekniklerinin dentine olan mikrogerilim bağlanma dayanımına (μTBS) etkisini değerlendirmektir. Gereç ve Yöntemler: Yetmiş iki adet çekilmiş çürüksüz insan üçüncü büyük azı dişi rastgele 18 gruba ayrıldı (n=4). Dentin yüzeyleri #600-grit silikon-karbit kağıt ile cilalandı. Kontrol grubunu Clearfil SE Bond [SE] (Kuraray), deney gruplarını iki aşamalı etch&rinse Adper Single Bond2 [SB] (3M ESPE) ve tek aşamalı self-etch Clearfil S3 Bond [S3] (Kuraray) oluşturdu. Test edilen adezivler, üretici firmanın talimatları [Kontrol] veya %2 Klorheksidin ile [CHX], veya iki kat adeziv uygulaması ile [x2K] veya ilave hidrofobik reçine tabaka [HR] ile uygulandı. Kompozit blok (Filtek Ultimate, 3M ESPE) tabaklama yöntemiyle oluşturuldu ve distile suda bekletildi (24 saat/37°C). 1mm2 reçine-dentin çubuklarına (Isomet) ayrılarak hemen [24sa] veya 37°C’de altı ay [6.ay] suda bekletildikten sonra μTBS testine tabi tutuldu (Bisco) (Çapraz kafa hızı 0.5 mm/dk). Veriler Three-way ANOVA ve post-hoc Tukey testleri kullanılarak analiz edildi (p<0.05). Bulgular: Dentine μTBS değerleri; adeziv sisteme (p:0.001), adeziv uygulama tekniklerine (p:0.001), zamana (p:0.001) göre anlamlı farklılık göstermiştir. SE-Kontrol en yüksek μTBS gösterirken (p:0.001), SB-Kontrol ve S3-Kontrol arasında anlamlı farklılık bulunmamaktadır (p>0.05). Tüm grupların 6.ay µTBS değerleri anlamlı düzeyde azalmıştır (p<0.01). HR ve x2K, CHX ve Kontrol gruplarından anlamlı düzeyde daha yüksek μTBS sergilerken (p<0.01), CHX ve Kontrol arasında anlamlı bir fark bulunmamıştır (p>0.05). Adezivler karşılaştırıldığında 6.ayda, x2K ve HR uygulaması S3’de anlamlı düzeyde yüksektir (p<0.01). Sonuçlar: HR ve x2K dentine μTBS değerlerini arttırırken; CHX ile anlamlı düzeyde bir artış gözlenmemiştir. Anahtar kelimeler: Dental adezivler, dentin, dentin yapıştırma ajanları, klorheksidin, uzun ömürlülük

Anahtar Kelimeler:

dental adezivler, dentin, dentin yapıştırma ajanları, klorheksidin, uzun ömürlülük

Evaluation of different adhesive applications on dentine microtensile bond strength

Background: The aim of this study was to evaluate the effect of different adhesive applications on dentine microtensile bond strength (μTBS) in simplified adhesive systems. Methods: Seventy-two extracted non-carious human third molars were randomly divided 18 groups (n=4). Dentin surfaces were polished with #600-grit silicon-carbide paper. Two-step etch&rinse Adper Single Bond2 [SB](3M ESPE) and one-step self-etch Clearfil S3 Bond [S3](Kuraray) were tested, two-step self-etch Clearfil SE Bond [SE](Kuraray) was used as control. The tested adhesives were applied according to the manufacturer’s instructions [MI] or with 2% Chlorhexidine [CHX] or double application of the adhesive [DA] or with hydrophobic resin layer [HR]. Composite build-up (Filtek Ultimate,3MESPE) was restored incrementally and stored in distilled water (24h/37°C). Teeth were sectioned as 1mm2 resin-dentin sticks (Isomet) and subjected to μTBS test (Bisco) immediately [24h] or after six months of water-storage [6M] (Crosshead speed:0.5mm/min.) Data were analyzed by Three-way ANOVA and post-hoc Tukey’s tests (p<0.05). Results: The dentine µTBS was significantly influenced by the adhesive system (p:0.001), adhesive applications (p:0.001), and storage time (p:0.001). The highest μTBS was found in SE-MI (p<0.05), while the interaction between SB-MI and S3-MI was not significant (p>0.05). The µTBS of all groups decreased significantly at 6M (p<0.01). HR and DA showed significantly higher μTBS than CHX and MI (p<0.01), while no significant difference was found between CHX and MI (p>0.05). When adhesives were compared, DA and HR were significantly higher μTBS in S3 (p<0.01). Conclusion: HR and DA improved the μTBS while no significant enhancement was observed by CHX. Keywords: Chlorhexidine, dental adhesives, dentin, dentin bonding agents, longevity.

Keywords:

chlorhexidine, dental adhesives, dentin, dentin bonding agents, longevity,

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