Periimplantal Kemik Defektlerinin Titanyum Tüplerde Hazırlanan Trombositten Zengin Fibrin ile Kemik Rejenerasyonunun Histomorfometrik Değerlendirmesi: Tavşan Tibiasinda Deneysel Çalışma

Amaç: Bu çalışmanın amacı titanyum tüplerde hazırlanan trombositten zengin fibrinin (T-PRF) deneysel olarak hazırlanmış periimplantal kemik defektlerindeki rejenerasyonu incelemektir. Gereç ve Yöntem: Çalışmada 12 adet erkek wistar rat kullanılmış olup her bir tavşanın sağ ve sol tibiasına trephan frez ile 7 mm genişliğinde 4 mm derinliğinde defektler oluşturulmuştur. Daha sonra 3,3X8 mm lik implantlar tibialara yerleştirilmiş olup periimplantal defektler sırasıyla Otojen greft, T-PRF, Bifazik Kalsiyum Fosfat greft (BCP) ile restore edilmiştir. Kontrol grubu boş bırakılıp spontan iyileşmeye bırakılmıştır. 4 hafta sonra tüm tavşanlar ötenazi ile öldürülerek implant ile beraber etrafındaki kemik alındı. Kemik-implant kontağı toludin mavisi ile boyanan kesitlerin histomorfometrik incelenmesi ile değerlendirilmiştir. Bulgular: Otojen grupta (5267,50±228,95) kemik implant kontağı değeri en yüksek çıkmıştır. Ancak otojen grup ile T-PRF grubu (3932,50±275,50) arasında istatistiksel olarak bir fark gözlenmemiştir. BCP grubu ile kontrol grubunda en düşük BIC değerleri gözlenmiştir. Sonuçlar: Peri-implantal defektlerin rejenerasyonunda T-PRF grubunda neredeyse otojen grup kadar BIC değerleri bulunmuştur. Bu yüzden T-PRF gelecekte yönlendirilmiş kemik rejenerasyonunda otojen matriks fonksiyonu görevi görebilir.

Anahtar Kelimeler:

Titanyum Trombositten zengin fibrin, periimplantal defekt

Histomorphometric Evaluation of Bone Regeneration in Peri-Implant Osseous Defects Treated With Titanium Prepared Platelet Rich Fibrin: An Experimental Study in a Rabbit Model

Aim: The present study aims to investigate the healing of artificially created peri-implant osseous defects using Titanium-Platelet Rich Fibrin Material and Method: Bone defects (9-mm diameter, 4-mm depth) were created and implant beds (3-mm diameter, 6-mm depth) were prepared in the middle of them in rabbit tibias (24 rabbits) used as the experimental model. Afterwards, dental implants were installed into the left and right tibia (diameter 3.0 mm, length 8 mm). In the experimental groups, the peri-implant defect was filled with Autogenous grafts (AG), Titanium-Platelet Rich Fibrin (T-PRF), Biphasic Calcium Phosphate grafts (BCP). The control group did not receive any filling. The bone-to-implant contact (BIC) was obtained by histomorphometrically examining the sections taken from the study groups. Results: AG group was resulted in a high degree of BIC. No difference of statistical significance was found for the BIC as far as autogenous graft and PRF groups are concerned. Conclusion: Peri-implantal defects in regeneration of T-PRF were found to be nearly as affective as the autogenous bone graft. We, therefore, conclude that T-PRF can be used for guided bone regeneration in the function of autogenic matrix in the future.

Keywords:

Titanium-prepared-Platelet-Rich-Fibrin Periimplantal defect, Bone-to-implant contact,

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