Merve AKKUŞ, Faruk KILIÇ, Halil AŞCI, Fatma Nihan CANKARA, Mehtap SAVRAN, Dilek ULUSOY KARATOPUK

OLANZAPİN VE L-KARNİTİNİN RAT TESTİS DOKUSU ÜZERİNDEKİ ETKİSİNİN ARAŞTIRILMASI

Amaç Olanzapinin cinsel işlev bozukluğu üzerine olumsuz etkileri vardır. Üreme sisteminde de yapısal değişikliklere neden olduğunu gösteren sınırlı sayıda çalışma bulunmaktadır. Bu çalışmada Olanzapinin neden olduğu testis hasarı üzerine L-Karnitin'in etkilerinin histopatolojik, sperm parametreleri, biyokimyasal açıdan incelenmesi amaçlanmıştır. Gereç ve Yöntem Çalışmamızda toplam 48 adet erişkin Sprague-Dawley erkek sıçan kullanıldı. Sıçanlar, her grupta 8 sıçan olacak şekilde 6 gruba ayrıldı: Kontrol grubu (C), 200 mg/kg L-Karnitin uygulanan grup (LC), 2 mg/kg düşük doz Olanzapin verilen grup (LOZN), 2 mg/kg Olanzapin ve 200 mg/kg L-Karnitin verilen grup (LOZN+LC), 4 mg/kg Olanzapin verilen grup (HOZN), 4 mg/kg Olanzapin ve 200 mg/kg L-Karnitin verilen grup (HOZN+ LC). MDA, IL-1B, IL-6, TAS, TOS düzeylerinin belirlenmesi, histolojik değerlendirme için testis dokuları kullanıldı. Bulgular Olanzapin alan gruplarda sperm sayısında azalma, MDA, IL-1β, TOS değerlerinde artış, testis dokusunda doza bağlı histopatolojik değişiklikler gözlendi. Olanzapin ve L-Karnitin uygulanan gruplarda sadece Olanzapin verilen gruplara göre histopatolojik değişiklikler daha düşük oranda bulundu. Sonuç Yüksek doz Olanzapin uygulanan gruplarda gözlenen testis yapısındaki dejeneratif histolojik bulguların, Olanzapinin testis dokusunda oluşturduğu oksidatif stresten kaynaklanabileceği sonucuna varıldı. L-Karnitin ise oksidatif hasarı azaltarak testis dejenerasyonun azaltılmasında etkili olabileceği düşünüldü.

Anahtar Kelimeler:

Olanzapin, L-Karnitin, testis, sperm, oksidatif stres

INVESTIGATION OF THE EFFECT OF OLANZAPINE AND L-CARNITINE ON RAT TESTIS TISSUE

Objective Olanzapine has adverse effects on sexual dysfunction. There are a limited number of studies showing that it also causes structural changes in the reproductive system. This study aimed to examine the effects of L-Carnitine on testicular damage caused by Olanzapine in terms of histopathological, sperm parameters, and biochemical aspects. Material and Method A total of 48 adult Sprague-Dawley male rats were used in our study. Rats were divided into 6 groups, 8 rats in each group: the Control group (C), 200 mg/ kg L-Carnitine administered group (LC), 2 mg/kg low dose Olanzapine administered group (LOZN), 2 mg/kg Olanzapine, and 200 mg/kg L-Carnitine administered group (LOZN+LC), 4 mg/kg Olanzapine administered group (HOZN), 4 mg/kg Olanzapine and 200 mg/ kg L-Carnitine administered group (HOZN+LC). Testicular tissues were used for the determination of MDA, IL-1B, IL-6, TAS, TOS levels, and histological evaluation. Results In the groups receiving Olanzapine, a decrease in sperm count, an increase in MDA, IL-1β, TOS values, and dose-dependent histopathological changes in testicular tissue were observed. Histopathological changes were found at a lower rate in the Olanzapine and L-Carnitine administered groups compared to the Olanzapine-only groups. Conclusion It was concluded that the degenerative histological findings in the testicular structure observed in the high-dose Olanzapine administered groups might be caused by the oxidative stress induced by Olanzapine in the testicular tissue. L-Carnitine, on the other hand, was thought to be effective in reducing testicular degeneration by reducing oxidative damage.

Keywords:

Olanzapine, L-Carnitine, testis, sperm, oxidative stress,

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