İlter İLHAN, Duygu DOGUC, Muhammet Yusuf TEPEBAŞI, Okan SANCER, Halil İbrahim BÜYÜKBAYRAM

GIDA KATKI MADDESİ SODYUM BENZOATIN OKSİDATİF STRES VE GENOTOKSİSİTE ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Amaç Son yıllarda gıda katkı maddelerinin kullanımında belirgin artış vardır. Doğumdan ölüme kadar maruz kalınan bu maddelerin, insanlarda uzun süreli tüketime bağlı olarak oluşturabileceği yan etkiler oldukça önem kazanmıştır. Çalışmamızda özellikle puberte öncesi sıçanlarda sodyum benzoata maruziyetin oksidatif stres ve genotoksisite açılarından değerlendirilmesini amaçladık. Gereç ve Yöntem Kırk iki adet, 4 haftalık erkek sıçan, kontrol (n=14), deney 1 (Acceptable Daily Intake (ADI) dozunda sodyum benzoat, n=14) ve deney 2 (No Advers Effect Level (NOAEL) dozunda sodyum benzoat, n=14) grupları şeklinde 3 gruba ayrılmıştır. Altı hafta süresince oral gavaj uygulanmıştır. Çalışma sonunda sakrifiye edilen sıçanların kan örneklerinden biyokimyasal parametreler (glukoz, kreatinin, AST, ALT, ALP, lipid profili, total protein, albümin), total oksidan status (TOS), total antioksidan status (TAS), iskemi modifiye albümin (İMA) ölçülmüş ve Comet yöntemi ile DNA hasarı değerlendirilmiştir. Ölçülen TOS ve TAS parametrelerinden oksidatif stres indeksi (OSİ) hesaplanmıştır. Bulgular NOAEL dozu sodyum benzoat uygulanan sıçanlarda ALT ve kreatinin seviyeleri yükselmiştir. Ayrıca TOS, IMA ve OSİ seviyeleri NOAEL grubunda yüksek iken TAS seviyeleri düşük bulunmuştur. Bunlara ek olarak NOAEL dozunda sodyum benzoat DNA hasarına yol açmıştır. Sonuç Çalışmamızda, prepubertal dönem sıçanlarda ADI dozunda sodyum benzoat uygulamasının, olumsuz bir etkisi gözlemlenmemiştir. Diğer taraftan NOAEL dozunda sodyum benzoat uygulamasının böbrek ve karaciğer fonksiyonlarını bozduğunu ve oksidatif stres ile genotoksisiteye yol açabileceği ortaya konmuştur.

Anahtar Kelimeler:

Comet, Gıda katkı maddesi, Oksidatif stres, Sodyum benzoat

INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

Objective Recently, there has been a significant increase in the use of food additives. Generation of possible side effects depending on the long-term consumption of these substances from birth to death has gained importance in humans. In our study, we aimed to evaluate the exposure to sodium benzoate in terms of oxidative stress and genotoxicity, especially in prepubertal rats. Material and Method In our study, 42 male rats (4 weeks-old) divided into three groups as the control (n=14), experiment 1 (sodium benzoate Acceptable Daily Intake (ADI) dose, n=14) and experiment 2 (sodium benzoate No Advers Effect Level (NOAEL)dose, n=14) groups and sodium benzoate was administration by oral gavage for 6 weeks. At the end of the study, rats were sacrified and biochemical parameters (glucose, creatinine, AST, ALT, ALP, lipid profile, total protein, albumin), total oxidant status (TOS), total antioxidant status (TAS), ischemia modified albumin (IMA) were measured, and DNA damage was evaluated by Comet analysis. Oxidative stress index (OSI) was calculated from the TOS and TAS parameters. Results ALT and creatinine levels were increased with the NOAEL dosage of sodium benzoate. In addition, TOS, IMA and OSI levels were elevated, while TAS levels were decreased in the NOAEL group. Also, NOAEL dose of sodium benzoate significantly caused DNA damage. Conclusions In our study, no adverse effect was observed in the administration of sodium benzoate at the dose of ADI in prepubertal rats. On the other hand, we found that NOAEL dose of sodium benzoate impairs kidney and liver functions and causes oxidative stress and genotoxicity.

Keywords:

Comet, Food additive, Oxidative stress, Sodium benzoate,

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