Fadime MUTLU İÇDUYGU, Egemen AKGÜN, Ebru ALP, Sibel DOĞUİZİ, Murat Atabey ÖZER

MMP9 −1562 C/T POLİMORFİZMİ, TÜRK TİP 2 DİABETES MELLİTUS HASTALARINDA ARTMIŞ DİYABETİK RETİNOPATİ RİSKİ İLE İLİŞKİLİDİR

Amaç Tip 2 diabetes mellitus (T2DM) ve diyabetin mikrokomplikasyonlarından olan diyabetik retinopati (DR) gelişimi genetik faktörlerden etkilenmektedir. Matriks metalloproteinazlar (MMPs), ekstraselüler matriks proteinlerinin yeniden şekillendirilmesi ve anjiyogenez gibi pek çok hücresel proseste rol alırlar. Bu çalışmada Türk toplumunda MMP9 −1562 C/T (rs3918242) polimorfizmi ile T2DM ve DR gelişim riski arasındaki ilişkinin araştırılması amaçlanmıştır. Gereç ve Yöntem Mevcut çalışmaya 168 DR’si olan T2DM hastası, 168 DR’si olmayan T2DM hastası ve 174 kontrol olmak üzere toplam 510 birey dahil edilmiştir. Genotipleri belirlemek amacıyla Polimeraz zincir reaksiyonu-Restriksiyon fragment uzunluk polimorfizmi (PCR-RFLP) yöntemi kullanılmıştır. Bulgular T2DM gelişimi ile MMP9 −1562 C/T polimorfizmi arasında bir ilişkiye rastlanmazken, CT ve CT+TT genotipleri ile T (p=0,001) alleli artmış DR riski ile ilişkili bulunmuştur. Ayrıca CT (p=0,010) ve CT+TT (p=0,015) genotip sıklığı proliferatif diyabetik retinopati (PDR) hastalarında proliferatif olmayan diyabetik retinopati (NPDR) hastalarına kıyasla yüksek bulunmuş, fakat regresyon analizi sonrasında sadece insülin kullanımının (p=0,003) PDR gelişimi ile ilişkili olduğu belirlenmiştir. Sonuç Elde ettiğimiz veriler Türk T2DM hastalarında MMP9 −1562 C/T polimorfizminin DR gelişimi ile ilişkili olduğunu, fakat bu polimorfizm ile DR şiddeti ve T2DM gelişimi arasında anlamlı bir ilişki bulunmadığını göstermektedir.

Anahtar Kelimeler:

Diabetik retinopati, MMP9, Polimorfizm, Proliferatif diyabetik retinopati, Tip 2 diabetes mellitus

THE MMP9 −1562 C/T POLYMORPHISM IS ASSOCIATED WITH INCREASED RISK OF DIABETIC RETINOPATHY IN TURKISH TYPE 2 DIABETES MELLITUS PATIENTS

Objective Type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) development is affected by genetic factors. Matrix metalloproteinases (MMPs) are involved in many cellular processes, such as remodeling of extracellular matrix proteins and angiogenesis. In this study, we investigated the relationship between MMP9 −1562 C/T (rs3918242) polymorphism and the development of T2DM and DR in Turkish population. Material and Method A total of 510 participants, involving 168 T2DM cases with DR, 168 T2DM cases without DR, and 174 controls, were included in the study. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was carried out to identify genotypes. Results The CT, CT+TT genotypes and the T allele of the MMP9 −1562 C/T polymorphism were associated with increased risk of DR (p=0.001). On the other hand, no relationship was found between the development of T2DM and this polymorphism. In addition, CT (p=0.010) and CT+TT (p=0.015) genotype frequencies were found to be higher in proliferative diabetic retinopathy (PDR) cases compared to nonproliferative diabetic retinopathy (NPDR) cases, but after regression analysis, only insulin use (p=0.003) was found to be associated with the development of PDR. Conclusion Our data show that the MMP9 −1562 C/T polymorphism is associated with the development of DR in Turkish T2DM patients, but no significant relationship was found between this polymorphism and the severity of DR and the development of T2DM.

Keywords:

MMP9, Diabetic retinopathy, proliferative diabetic retinopathy, type 2 diabetes mellitus, polymorphism,

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