Serap Keskin Tunç, Cennet Neslihan Eroğlu, Sevinç Şahin

HIV NEGATİF BİREYLERİN DENTAL FOLİKÜLLERINDE PATOLOJİK DEĞİŞİM RİSKİ AÇISINDAN HSV1, HSV2, HPV, HPV16, EBV VE HHV8 MARKIRLARININ ARAŞTIRILMASI

Giriş: Literatürde çeşitli virüslerin ağız kanserlerinin patogenezinde rol aldığı öne sürülmektedir. Ancak bu konu henüz tam olarak açıklanamamıştır. Bu çalışmanın amacı gömülü diş foliküllerinde olası prekanseröz viral markırların (HPV, HHV8, HSV1, HSV2, and EBV) varlığının araştırılmasıdır. Materyal ve Metod: 18 yaşından büyük 100 gönüllü hasta araştırmaya dahil edildi. Gömülü diş çekimi sonrasında diş folikülü çıkartılarak %10’luk formaldehit içinde fikse edildi. Histopatolojik ve immünohistokimyasal araştırma için HPV (HPV 8, HPV 11 ve HPV 18), p16 (HPV 16), HHV8, HSV1, HSV2, EBV antikorlar kullanılmıştır. Ayrıca immünohistokimyasal sonuçların klinikopatolojik veriler (yaş, cinsiyet ve sigara içme durumu) ile ilişkisi Ki-Kare Testi ile değerlendirilmiştir. 55 erkek ve 45 kadın araştırmaya dahil edildi. Bulgular: Araştırmaya katılan hastaların yaşları 17-56 (ortalama:25) arasında değişmekteydi. Histopatolojik olarak inflamasyon, granülasyon dokusu ve psodöepitelyomatöz hiperplazi varlığı araştırıldı. Displazi veya neoplaziye rastlanmadı. İmmünohistokimyasal boyamada p16 %62 oranında, EBV %32 oranında ve HSV-1 %26 oranında pozitiflik saptanmıştır. Tüm vakalarda HPV, HSV-2 ve HHV-8 immünonegatiftir. Bu bilinen diş folikülünde HPV 16, EBV ve HSV1 varlığını gösteren ilk çalışmadır. Sonuç: Bu virüslerin gömülü diş foliküllerinde tropizmi göstermek için rezervuar olarak işlev gördüklerini ileri sürebiliriz. Herhangi bir displazi veya neoplastik değişim tespit edilmemesine karşın viral etkilerin (özellikle HPV16 ve EBV için) uzun süre gömülü kalan dişlerde displazi ve neoplazm için tehdit olarak kabul edilebilir. Sonuç olarak olası viral onkogenezi ve tümörgenezi önlemek için gömülü kalan dişlerin çekimi yapılmalı ve sonrasında tüm foliküllerin histopatolojik incelenmesi yapılmalıdır.

Anahtar Kelimeler:

diş, germ, HPV, p16, HSV2, HSV1, EBV, HHV

INVESTIGATION OF HSV1, HSV2, HPV, HPV16, EBV AND HHV8 MARKERS IN TERMS OF THE PATHOLOGICAL CHANGES IN DENTAL FOLLICLES OF HIV NEGATIVE PERSONS

Several viruses have been suggested to play a role in the pathogenesis of oral cancers in the literature. However, this issue has not yet been clarified. The aim of this study is to investigate the presence of possible precancerous viral markers (HPV, HHV8, HSV1, HSV2 and EBV) in the impacted teeth follicles. Material and Method 100 patient aged 18 years or older was included in the study. Following the tooth extraction, the dental follicle was removed and fixed in 10% formaldehyde. HPV (HPV 8, HPV 11 and HPV 18), p16 (HPV 16), HHV8, HSV1, HSV2 and EBV antibodies were used for histopathological and immunohistochemical studies. In addition, the immunohistochemical results were evaluated by Chi-square test in relation to clinicopathological information (age, sex and smoking status). Total of 55 men and 45 women were included in the survey. Results The age of the patients who participated in the study ranged between 17 and 56 (mean: 25). Histopathologically, inflammation, granulation tissue and pseudoepitheliomatous hyperplasia were investigated. No dysplasia or neoplasm was found. Immunohistochemical staining showed p16% 62, EBV 32% and HSV-1 26% positivity. In all cases HPV, HSV2 and HHV-8 are immunonegative. It is the first study to show the presence of HPV 16, EBV and HSV1 in dental follicles. Conclusion We can claim that these viruses can act as reservoirs to show tropism in dental follicles. Although dysplasia or neoplastic changes were not detected in this study, viral effects (especially for HPV16 and EBV) may be seen as a threat leading to dysplasia and neoplasia in long term impacted wisdom teeth . As a result, for the possible viral oncogenesis and tumorigenesis, the impacted teeth should be removed and histopathologic examination of all follicles should be performed.

Keywords:

Dental follicle, HPV, p16, HSV1, HSV2, EBV, HHV8,

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