Tuncay Varol, E.Oğuzhan Oğuz, Enis Cezayirli, H.Seda Vatansever

Cerebral morphology in adult mice following Long-term gravity increase

Süleyman Demirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2007 Aralık; 14(4) Cerebral morphology in adult mice following Long-term gravity increase Tuncay Varol*, E. Oguzhan Oguz**, Enis Cezayirli*, H.Seda Vatansever*** *Department of Anatomy, Medical School, Celal Bayar University, Manisa, Turkey. **Department of Histology and Embryology, Medical School, Pamukkale University, Denizli, Turkey. ***Department of Histology and Embryology, Medical School, Celal Bayar University, Manisa, Turkey. Özet Uzun süreli gravite artışı uygulanan erişkin farelerde beyin morfolojileri Gravitenin akselerasyonu ve rotasyonunda yada parabolik uçuş sırasında artması organizma içerisinde strese neden olur. Bununla beraber, organizma içerisindeki bazı biyolojik değişiklikler artmış graviteye karşı cevap olarak gelişir. Uzun süreli gravite değişikliklerin ve rotasyonun etkilerini belirlemek amacı için, C57BL6 F1 fare beyin dokusunda hipergravite ortamındaki yapısal değişiklikler incelendi. Farelere bir veya iki gravite ortamında uzun-süreli sentrifüj uygulandı ve tedavi edilmeyen grup kontrol grubu olarak kabul edildi. Sentrifüjden 4 hafta sonra, fareler sakrifiye edildi ve beyinleri ascending aortadan %10 formalin solüsyonu ile perfüze edildi. Beyinler çıkarıldıktan sonra, parafine gömüldüler ve seri koronal kesitler ve sistemik üniform rastgele beyin kesitleri analiz edildi. Buna ilaveten, farklı gravite şartlarının nörodejeneratif etkilerini incelemek amacı için glial fibrillary asidik proteinin (GFAP) beyindeki immunohistokimyasal dağılımı incelendi. Sonuçlarımız göstermiştir ki, uzun süreli hipergravite beyin volumünde değişiklik yapmamaktadır ve tüm gruplarda beyinin hücresel morfolojisi normaldir ve dejeneratif değişiklikler gözlenmemiştir. Farelerin beyin morfolojilerinin uygulanan bu şartlarda hipergravite ile etkilenmediği gözlenmiştir. Anahtar Kelimeler: C57BL6 F1 fare, uzun-süreli sentrifüj, Cavalieri'nin volüm ölçümü, GFAP Abstract Cerebral morphology in adult mice following Long-term gravity increase Increases in gravitational forces that result from acceleration and rotation or parabolic flight can create significant stress for living organisms. Indeed, some biological changes in living organisms have specifically arisen to combat the effects of increased gravitational forces. To determine the potential effects of rotation and long-term gravitational changes, we have investigated the structural changes in C57BL6 F1 mice cerebral tissue under hypergravity conditions. Mice were subjected to long-term centrifugation under one or two gravities and compared with a non-treated control group. After 4 weeks of centrifugation, the mice were sacrificed and their brains were perfused through the ascending aorta with 10% formaldehyde. After removal of the brains, they were embeded in paraffin embedding and the cutting of serial coronal sections and systematic uniform random cerebral sections were analysed and The sterologic cortex and medulla volume estimations were performed. In addition, the immunohistochemical distribution of glial fibrillary acidic protein (GFAP) in cerebrum was determined to reveal any neurodegenerative effects of these different gravity conditions. Our results demonstrate that there were no long-term hypergravitational effects upon the cerebral volume, and that the cellular morphology of the cerebrum in all of the groups remained normal, and hence free from any degenerative changes. Under given conditions mice cerebral morphology has not been effected by hypergavity. Keywords: cerebrum , C57BL6 F1 mice, long-term centrifugation, Cavlieri's volume estimation, GFAP

Anahtar Kelimeler:

C57BL6 F1 fare, uzun-süreli sentrifüj, Cavlieri'nin volüm ölçümü, GFAP

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