Deniz ÖZTÜRK, Ayhan TANYELİ, Derya GÜZEL, Mustafa Can GÜLER, Ersen ERASLAN, Hüseyin BAYLAN

Mirisetinin Testiküler İskemi Reperfüzyon ile İndüklenen Testis ve Akciğer Hasarına Karşı Etkileri

Amaç: Testis torsiyonu birkaç saat içinde tedavi edilmezse erkeklerde testis atrofisine ve kısırlığa neden olabilir. Antioksidan ajanlar testis torsiyonu ile baş etmede hedefler arasındadır. Mirisetin (myricetin, 3, 3, 4, 5, 5, 7-hekzahidroksiflavon, MYR), antioksidan, antikanser, anti-inflamatuar, antiviral ve antidiyabetik aktivitelere sahiptir. Gereç ve Yöntemler: 32 adet Wistar albino erkek sıçan rastgele 4 gruba (n=8) ayrıldı. Grup I sham grup olarak tanımlandı. Grup II, I/R grubunda testis torsiyonu yapıldı. Grup III (MYR 25) ve grup IV'te (MYR 50), detorsiyondan 30 dakika önce 25 ve 50 mg/kg dozlarda MYR intraperitoneal olarak uygulandı. Testis ve akciğer doku örnekleri biyokimyasal olarak incelendi. Bulgular: Testis ve akciğer dokularında MYR, I/R grubuna kıyasla hem yüksek hem de düşük dozlarda TOS, MPO ve MDA seviyelerini azaltmıştır. Ayrıca MYR uygulanan gruplarda I/R grubuna kıyasla SOD değerleri artmıştır. Sonuç: Biyokimyasal parametreler değerlendirildiğinde, MYR testis I/R hasarını takiben testis ve akciğer dokuları üzerinde olumlu etkiler gösterdi.

Anahtar Kelimeler:

mirisetin, torsiyon detorsiyon, iskemi reperfüzyon, testis, akciğer

The Effects of Myricetin Against Testicular and Lung Injury Induced by Testicular Ischemia Reperfusion Model

Objective: Testicular torsion may lead to testicular atrophy and male infertility if untreated within some hours. Since ischemia reperfusion (I/R) injury is a reason for the harmful effects during testicular torsion, antioxidant agents are among the targets of science to deal with reactive oxygen species (ROS). As a flavonoid member, myricetin (3, 3, 4, 5, 5, 7-hexahydroxyflavone, MYR) has antioxidant, anticancer, anti-inflammatory, antiviral, and antidiabetic activities. Materials and Methods: In this experiment 32 Wistar albino male rats were randomly divided into 4 groups (n=8). Group I was defined as the sham group. In group II, I/R group, testicular torsion detorsion was performed. In group III (MYR 25) and group IV (MYR 50), MYR was administrated intraperitoneally at 25 and 50 mg/kg doses, 30 minutes before detorsion. The testicular and lung tissue samples were examined biochemically. Results: In both testis and lung tissues, MYR decreased TOS, MDA, and MPO levels at both high and low doses compared to the I/R group. Besides, SOD values increased in MYR treatment groups compared to the I/R group. Conclusion: MYR performed promising effects on testicular and lung tissues following testicular I/R injury according to biochemical parameters.

Keywords:

myricetin, torsion detorsion, ischemia reperfusion, testis, lung,

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