Derya GÜZEL, Ayhan TANYELİ, Songül DOĞANAY, Mustafa Can GÜLER, Ersen ERASLAN

Urapidil Çekal Ligasyon ve Delme ile İndüklenen Akciğer ve Böbrek Hasarını Hafifletir mi?

Amaç: Burada, çekal ligasyon ve ponksiyonun (CLP) neden olduğu akciğer ve böbrek yaralanmalarına karşı Urapidil'in olası koruyucu etkileri araştırıldı. Materyal ve Metod: 32 Sprague Dawley erkek sıçan sham, CLP, Dimetil sülfoksit (DMSO) ve Urapidil (URA) 0.5 mg/kg şeklinde 4 gruba ayrıldı. Akciğer ve böbrek dokularında çeşitli oksidan, inflamatuvar ve antioksidan parametreler belirlendi. Bulgular: CLP ve DMSO gruplarında sham grubuna göre oksidan ve inflamatuvar biyobelirteçler artarken antioksidan biyobelirteçler azalmıştır. Tedavi grubunda CLP ve DMSO gruplarına göre antioksidan parametreler artarken oksidan ve inflamatuvar biyobelirteçler azaldı. Sonuç: Mevcut sonuçlar, URA uygulamasının CLP ile ilişkili polimikrobiyal sepsis modelinin neden olduğu akciğer ve böbrek hasarlarına karşı etkili olduğunu göstermiştir.

Anahtar Kelimeler:

urapidil, çekal ligasyon ve delme, akciğer, böbrek, sıçan

Does urapidil alleviate lung and renal injuries induced by cecal ligation and puncture?

Objective: Here, possible protective effects of Urapidil were investigated against lung and renal injuries induced by cecal ligation and puncture (CLP). Materials and Methods: 32 Sprague Dawley male rats were assigned to 4 groups as follows; sham, CLP, DMSO and Urapidil (URA) 0.5 mg/kg. Several oxidant, inflammatory and antioxidant parameters were determined in lung and renal tissues. Results: Oxidant and inflammatory biomarkers increased while antioxidant biomarkers decreased in CLP and DMSO groups compared to sham group. Antioxidant parameters increased while oxidant and inflammatory biomarkers declined in treatment group compared to CLP and DMSO groups. Conclusion: Present results have demonstrated that URA administration is effective against lung and renal injuries caused by CLP-related polymicrobial sepsis model.

Keywords:

urapidil, cecal ligation and puncture, lung, renal, rat,

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1. Harasstani OA, Tham CL, Israf DA. Kaempferol and Chrysin Synergies to Improve Septic Mice Survival. Molecules 2017;22(1).
2. Kumar S, Gupta E, Kaushik S, Kumar Srivastava V, Mehta SK, Jyoti A. Evaluation of oxidative stress and antioxidant status: Correlation with the severity of sepsis. Scand J Immunol 2018;87(4):e12653.
3. Jawad I, Lukšić I, Rafnsson SB. Assessing available information on the burden of sepsis: global estimates of incidence, prevalence and mortality. J Glob Health 2012;2(1):010404.
4. Hotchkiss RS, Karl IE. The pathophysiology and treatment of sepsis. N Engl J Med 2003;348(2):138-150.
5. Wang Z, Holthoff JH, Seely KA, Pathak E, Spencer HJ, Gokden N, et al. Development of oxidative stress in the peritubular capillary microenvironment mediates sepsis-induced renal microcirculatory failure and acute kidney injury. Am J Pathol 2012;180(2):505-516.
6. Galley HF. Oxidative stress and mitochondrial dysfunction in sepsis. Br J Anaesth 2011;107(1):57-64.
7. Peralta JG, Llesuy S, Evelson P, Carreras MC, Flecha BG, Poderoso JJ. Oxidative stress in skeletal muscle during sepsis in rats. Circ Shock 1993;39(2):153-159.
8. Graziani G, Buskermolen M, Oldani S, Brambilla G. [Sepsis, acute renal failure and multiple organ dysfunction syndrome]. Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia 2006;23 Suppl 36:S13-21.
9. Kaymak C, Basar H, Sardas S. Reactive oxygen species (ROS) generation in sepsis. FABAD J Pharm Sci 2011;36(1):41-47.
10. Dkhil MA, Kassab RB, Al-Quraishy S, Abdel-Daim MM, Zrieq R, Abdel Moneim AE. Ziziphus spina-christi (L.) leaf extract alleviates myocardial and renal dysfunction associated with sepsis in mice. Biomed Pharmacother 2018;102:64-75.
11. Wu GJ, Lin YW, Tsai HC, Lee YW, Chen JT, Chen RM. Sepsis-induced liver dysfunction was ameliorated by propofol via suppressing hepatic lipid peroxidation, inflammation, and drug interactions. Life sciences 2018;213:279-286.
12. Niki E, Yoshida Y, Saito Y, Noguchi N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem Biophys Res Commun 2005;338(1):668-676.
13. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38(12):1103-1111.
14. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004;37(4):277-285.
15. Goodman RB, Pugin J, Lee JS, Matthay MA. Cytokine-mediated inflammation in acute lung injury. Cytokine & growth factor reviews 2003;14(6):523-535.
16. Xiao XG, Zu HG, Li QG, Huang P. Sivelestat sodium hydrate attenuates acute lung injury by decreasing systemic inflammation in a rat model of severe burns. European review for medical and pharmacological sciences 2016;20(3):528-536.
17. Güzel Erdoğan D, Tanyeli A. Inhibition of NADPH oxidase attenuates sepsis induced acute lung oxidative damage in rats. Journal of Cellular Neuroscience & Oxidative Stress 2018;10(2).
18. Tanyeli A, Erdoğan Güzel D. Alliin mitigates Cecal Ligation Puncture (CLP)-induced lung injury through antioxidant and antiinflammatory effects. Turhish Journal of Sciences 2019;4(2):46-59.
19. Tanyeli A, Eraslan E, Güler MC, Şebin ÖS, Çelebi D, Özgeriş FB, et al. Investigation of biochemical and histopathological effects of tarantula cubensis D6 on lung tissue in cecal ligation and puncture-induced polymicrobial sepsis model in rats. Medicine Science International Medical Journal 2019;8(3):644-50.
20. dos Santos CC, Murthy S, Hu P, Shan Y, Haitsma JJ, Mei SH, et al. Network analysis of transcriptional responses induced by mesenchymal stem cell treatment of experimental sepsis. Am J Pathol 2012;181(5):1681-1692.
21. Öztürk D, Erdoğan Güzel D, Tanyeli A, Çomaklı S, Baylan H, Polat E. The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Turkish Journal of Biochemistry 2019;44(4):539-548.
22. Eraslan E, Tanyeli A, Polat E, Yetim Z. Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study. Journal of Cellular Biochemistry 2019;120(10):17159-166.
23. Bottorff MB, Hoon TJ, Rodman JH, Gerlach PA, Ramanathan KB. Pharmacokinetics and pharmacodynamics of urapidil in severe hypertension. J Clin Pharmacol 1988;28(5):420-426.
24. Shebuski RJ, Zimmerman BG. Suppression of reflex tachycardia following alpha-adrenoceptor blockade in conscious dogs: comparison of urapidil with prazosin. J Cardiovasc Pharmacol 1984;6(5):788-794.
25. Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal-Tissues by Thiobarbituric Acid Reaction. Anal Biochem 1979;95(2):351-358.
26. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 1982;78(3):206-209.
27. Sun Y, Oberley LW, Li Y. A Simple Method for Clinical Assay of Superoxide-Dismutase. Clin Chem 1988;34(3):497-500.
28. Vincent JL, Rello J, Marshall J, Silva E, Anzueto A, Martin CD, et al. International study of the prevalence and outcomes of infection in intensive care units. Jama 2009;302(21):2323-2329.
29. Hotchkiss RS, Monneret G, Payen D. Sepsis-induced immunosuppression: from cellular dysfunctions to immunotherapy. Nat Rev Immunol 2013;13(12):862-874.
30. Nuytinck HK, Offermans XJ, Kubat K, Goris JA. Whole-body inflammation in trauma patients. An autopsy study. Arch Surg 1988;123(12):1519-1524.
31. Aziz M, Ode Y, Zhou M, Ochani M, Holodick NE, Rothstein TL, et al. B-1a cells protect mice from sepsis-induced acute lung injury. Mol Med 2018;24(1):26.
32. Bagshaw SM, Uchino S, Bellomo R, Morimatsu H, Morgera S, Schetz M, et al. Septic acute kidney injury in critically ill patients: clinical characteristics and outcomes. Clin J Am Soc Nephrol 2007;2(3):431-439.
33. Pejić S, Todorović A, Stojiljković V, Kasapović J, Pajović SB. Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma. Reprod Biol Endocrinol 2009;7:149.
34. Tsiotou AG, Sakorafas GH, Anagnostopoulos G, Bramis J. Septic shock; current pathogenetic concepts from a clinical perspective. Med Sci Monit 2005;11(3):Ra76-85.
35. Mantzarlis K, Tsolaki V, Zakynthinos E. Role of Oxidative Stress and Mitochondrial Dysfunction in Sepsis and Potential Therapies. Oxid Med Cell Longev 2017;2017:5985209.
36. Walley KR, Lukacs NW, Standiford TJ, Strieter RM, Kunkel SL. Balance of inflammatory cytokines related to severity and mortality of murine sepsis. Infection and immunity 1996;64(11):4733-4738.
37. Bi J, Cui R, Li Z, Liu C, Zhang J. Astaxanthin alleviated acute lung injury by inhibiting oxidative/nitrative stress and the inflammatory response in mice. Biomed Pharmacother 2017;95:974-982.
38. Araújo CV, Estato V, Tibiriçá E, Bozza PT, Castro-Faria-Neto HC, Silva AR. PPAR gamma activation protects the brain against microvascular dysfunction in sepsis. Microvasc Res 2012;84(2):218-221.
39. Blackwell TS, Christman JW. Sepsis and cytokines: current status. Br J Anaesth 1996;77(1):110-117.
40. Yapca OE, Borekci B, Suleyman H. Ischemia-reperfusion damage. The Eurasian journal of medicine 2013;45(2):126-137.
41. Del Maestro RF. An approach to free radicals in medicine and biology. Acta physiologica Scandinavica Supplementum 1980;492:153-168.
42. Deng WS, Xu Q, Liu YE, Jiang CH, Zhou H, Gu L. Effects of melatonin on liver function and lipid peroxidation in a rat model of hepatic ischemia/reperfusion injury. Experimental and therapeutic medicine 2016;11(5):1955-1960.
43. Ozturk E, Demirbilek S, Begec Z, Surucu M, Fadillioglu E, Kirimlioglu H, et al. Does leflunomide attenuate the sepsis-induced acute lung injury? Pediatric surgery international 2008;24(8):899-905.
44. Gaut JP, Yeh GC, Tran HD, Byun J, Henderson JP, Richter GM, et al. Neutrophils employ the myeloperoxidase system to generate antimicrobial brominating and chlorinating oxidants during sepsis. Proceedings of the National Academy of Sciences of the United States of America 2001;98(21):11961-11966.
45. Rabus M, Demirbag R, Sezen Y, Konukoglu O, Yildiz A, Erel O, et al. Plasma and tissue oxidative stress index in patients with rheumatic and degenerative heart valve disease. Turk Kardiyoloji Dernegi arsivi : Turk Kardiyoloji Derneginin yayin organidir 2008;36(8):536-540.
46. Rangel-Frausto MS. The epidemiology of bacterial sepsis. Infect Dis Clin North Am 1999;13(2):299-312, vii.
47. Saxena PR, Villalon CM. Brain 5-HT1A receptor agonism: a novel mechanism for antihypertensive action. Trends Pharmacol Sci 1990;11(3):95-96.
48. Bopp C, Auger C, Diemunsch P, Schini-Kerth V. The effect of urapidil, an alpha-1 adrenoceptor antagonist and a 5-HT1A agonist, on the vascular tone of the porcine coronary and pulmonary arteries, the rat aorta and the human pulmonary artery. Eur J Pharmacol 2016;779:53-58.
49. Valenta B, Kotai E, Weisz E, Singer EA. Influence of urapidil and 8-OH-DPAT on brain 5-HT turnover and blood pressure in rats. J Cardiovasc Pharmacol 1990;15 Suppl 7:S68-74.