Deneysel Renal İskemi Reperfüzyon Modelinde Serbest Oksijen Radikali Temizleyici Olarak P-Kumarik Asit’in Koruyucu Etkisi

Amaç Bu çalışmanın amacı, sıçan böbrek dokusunda iskemi-reperfüzyona bağlı doku hasarı üzerine p-kumarik asit (p-CA)’in antioksidan etkisini değerlendirmektir. ( Sakarya Tıp Dergisi 2018, 8(3):625-631 ). Yöntem 12-16 haftalık 200-250 gr otuz iki adet Wistar Albino cinsi dişi sıçan temin edildi. Sıçanlara intraperitonal olarak ketamine (75 mg/kg) ve xylazine (8 mg/kg) anestezisi altında sırt bölgesinden yapılan bir kesi ile sağ nefrektomi uygulandı. Bu sıçanlar randomize olarak dört gruba eşit olarak bölündü (n=8). Gruplar; sham (S), renal iskemi-reperfüzyon (R-IR), R-IR ile 50 mg/kg p-kumarik asit (p-CA 50) ve R-IR ile 100 mg/kg p-kumarik asit (p-CA 100) uygulanan grup olarak sınıfl andırıldı. Sham hariç diğer gruplardaki sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan sonra sol renal arter klemplenerek iskemi ve reperfüzyon protokolü uygulandı. P-kumarik asit iskemiden 60 dk önce ve reperfüzyon başlangıcından 30 dk önce olmak üzere iki doz şeklinde uygulandı. İşlemler tamamlandıktan sonra sol böbrek total antioksidan düzeyi (TAS), total oksidan düzeyi (TOS), süperoksit dismutaz (SOD), malondialdehit (MDA) ve myeloperoksidaz (MPO) seviyeleri spektrofotometrik yöntemlerle ölçüldü. Oksidatif stres indeksi (OSI) hesaplandı. Bulgular R-IR grubunda S ile karşılaştırıldığında TAS ve SOD değeri düşerken, TOS, MDA, MPO ve OSI değerleri yükseldi (p

Protective Effect Of P-Coumaric Acid As Free Oxygen Radical Scavenger In Experimental Renal Ischemia-Reperfusion Model

Aim The aim of this study is to evaluate the antioxidant effect of p-Coumaric Acid (p-CA) on tissue damage due to ischemia-reperfusion in ratkidney tissue. ( Sakarya Med J 2018, 8(3):625-631)Methods Thirty-two 12-16 week-old Wistar Albino female rats weighing 200-250 g were used in this study. Rats underwent right nephrectomyintraperitoneally with an incision made in the dorsal region under ketamine (75 mg/kg) and xylazine (8 mg/kg) anesthesia. These rats wererandomly divided into four groups in equal numbers (n = 8). Groups were classifi ed as the sham (S), renal ischemia-reperfusion (R-IR),50 mg/kg p-CA with R-IR (p-CA 50), and 100 mg/kg p-CA with R-IR (p-CA 100). After the dorsum of all rats was opened and the rightnephrectomy was performed, all groups except sham were clamped on the left renal artery and ischemia and reperfusion protocol wasperformed. P-CA was administered in two doses, 60 min before ischemia and 30 min before reperfusion. Total antioxidant levels (TAS),total oxidant level (TOS), superoxide dismutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) levels of left kidney weremeasured spectrophotometrically, and oxidative stress index (OSI) was calculated.Results TOS, MDA, MPO, and OSI values increased in R-IR group compared to S (p

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