SİSTEMİK OLARAK KULLANILAN ZOLEDRONİK ASİTİN, DENEYSEL PERİODONTİTİS OLUŞTURULAN SIÇANLARDA İYİLEŞME POTANSİYELİ ÜZERİNE ETKİSİNİN İNCELENMESİ

Amaç: Bu çalışmada amacımız hayvan modelinde deneysel periodontitis oluşumundan sonra, periodontitis etkeni kaldırılmadan kullandırılan zoledronik asidin, periodontitis etkeni ortadan kaldırıldıktan sonra dokunun iyileşme potansiyeli üzerindeki etkisini histopatolojik olarak gözlemlemeyi hedeflemektir. Gereç ve Yöntem: Sprague-Dawley cinsi 30 adet yetişkin erkek sıçan bifosfanat kullanan ve kullanmayan olarak 2 gruba ayrılmıştır. Deney başlangıcında tüm sıçanlara, anestezi altında, sağ üst 1.molar dişlerinin etrafına 5.0 ipek dikiş yerleştirilmiştir. Sol üst 1. molar dişlere ise herhangi bir uygulama yapılmamıştır. Dikişlerin yerleştirilmesinden sonra 3 hafta beklenilmiştir. 21. günde hayvanlarda deneysel periodontitis gözlendikten sonra deney grubundaki hayvanlara kas içi 6 hafta boyunca 7,5uq/kg zoledronik asit enjekte edilmiştir. 0., 7., 14., 21., 28., ve 35.günlerde; 6 hafta boyunca, haftada bir, kas içi ilaç verilmesinden sonra deney grubu sıçanlarında, haftalık ağırlık takibi yapılmıştır ve not edilmiştir. Son ilaç enjeksiyonları tamamlanan deney grubu hayvanlarının ve aynı günlerde %0,9 serum fizyolojik uygulanan kontrol grubu hayvanlarının 6 hafta sonunda, anestezi altında yerleştirilen dikişleri kaldırılmıştır. İki haftalık iyileşme süresi için beklenilmiş ve bu iki hafta içinde de deney grubundaki hayvanlara zoledronik asit enjekte edilmiştir. Bulgular: Histolojik değerlendirme sonuçlarında zoledronik asit kullanılmış ve deneysel periodontitis oluşturulan grupta iltihap, nekroz, periodontal aralık ve epitel proliferasyonu istatiksel anlamlı p<0,05 olarak daha fazla bulunmuştur Sonuç: Klinik açıdan değerlendirildiğinde, bifosfanat türev ilaç kullandırılmış sıçanlarda, ilaç kullanımı öncesinde, var olan periodontitisin tedavi edilmesinin yara iyileşmesi üzerinde olumlu etkileri gözlenmiştir.

Anahtar Kelimeler:

Zoledronik asit, sıçan, deneysel periodontitis, osteonekroz, histoloji

THE EFFECT OF SYSTEMIC ZOLEDRONIC ACID ON THE HEALING POTENTIAL OF RATS WITH EXPERIMENTAL PERIODONTITIS

Objective: In this study, our goal was to observe the effect of zoledronic acid histopathologically, which is used without removing the periodontitis agent, on the healing potential of the tissue after the experimental periodontitis formation in an animal model. Material and Method: 30 adult male Sprague-Dawley rats were divided into 2 groups as bisphosphonate users and non-users. At the beginning of the experiment, all rats were put under anesthesia, and 5.0 silk sutures were placed around their right upper first molars. No suture was placed around the left upper first molar teeth. It was waited for 3 weeks after the placement of the sutures. After experimental periodontitis was observed in the animals on the 21st day, 7.5uq/kg zoledronic acid was injected intramuscularly for 6 weeks in the animals in the experimental group. After intramuscular drug administration once a week for 6 weeks, weekly weight monitoring was performed on days 0, 7, 14, 21, 28, and 35 and noted in the experimental group rats. At the end of 6 weeks, the sutures were removed under the anesthesia from the experimental group animals whose last drug injections were completed and the control group animals that were administered 0.9% saline on the same days. A recovery period of two weeks was expected after which all animals were sacrificed. Results: Zoledronic acid was used in the histological evaluation results, and experimental inflammation, necrosis, periodontal space and epithelial proliferation in the group with periodontitis statistically significant p<0.05 was found to be higher. Conclusion: When evaluated clinically, positive effects on wound healing were observed in rats treated with bisphosphonate-derived drugs by treating existing periodontitis prior to drug administration.

Keywords:

Zoledronic acid, rat, experimental periodontitis, osteonecrosis, histology,

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