Helichrysum plicatum Çiçeklerinin Su, Etanol, Aseton, Kloroform ve Hekzan Ekstrelerinin Antioksidan Aktivitelerinin Belirlenmesi

Helichrysum plicatum Asteracae familyasından olup tek yıllık çiçekli bir bitki olup, halk arasında çeşitli rahatsızlıkların tedavisinde yaygın olarak kullanılmaktadır. Anadolu’da genel olarak “mantuvar, ölmez çiçek ve altın otu” olarak bilinen Helichrysum böbrek taşlarını düşürmek amacıyla, soğuk algınlığı, şeker hastalığı ve mide rahatsızlıklarına karşı da kullanılmaktadır. Bu çalışmada, Helichrysum plicatum’un çiçeklerinin farklı polariteye sahip çözücülerle (su, etanol, aseton, kloroform, hekzan) hazırlanmış ekstrelerinin antioksidan aktiviteleri araştırılmıştır. Ekstrelerin farklı konsantrasyonlardaki (10-80 μg/ml) antioksidan aktiviteleri, Bakır (II) indirgeme (CUPRAC), ABTS (2,2’-azinobis-(3-etilbenzotiazolin–6-sülfonik asit)) ve DPPH (1,1-Difenil 2-pikril hidrazil) yöntemleriyle belirlenmiş olup, sonuçlar BHT, BHA ve Troloks standart antioksidan maddelerle karşılaştırılarak değerlendirilmiştir. Bitkinin farklı çözücülerle elde edilen ekstrelerine ait antioksidan aktivitelerinin, standart antioksidan maddelerden daha düşük olduğu tespit edilmiştir. Bununla birlikte CUPRAC ve DPPH yöntemlerinde su ekstresi, ABTS yönteminde ise etanol ekstresi, diğer ekstrelerden daha yüksek antioksidan aktivite göstermiştir. Bu sonuçlar, Helichrysum plicatum’un çiçek özütlerinin kozmetik, farmasötik ve gıda endüstrilerinde çeşitli uygulamalar için ulaşılabilir doğal antioksidan kaynaklar olduğunu ortaya koymuştur.

Anahtar Kelimeler:

Helichrysum plicatum, Antioksidan aktivite, CUPRAC, ABTS, DPPH

Determination of Antioxidant Activities of Water, Ethanol, Acetone, Chloroform and Hexane Extracts of Helichrysum plicatum Flowers

Helichrysum plicatum is an annual flowering plant from the Asteraceae family and is widely used in the treatment of various ailments among the people. Helichrysum, which is generally known as " mantuvar, ölmez çiçek and altın otu " in Anatolia, is also used against colds, diabetes and stomach ailments in order to reduce kidney stones. In this study, antioxidant activities were investigated in flower extracts of Helichrysum plicatum prepared with solvents of different polarities (water, ethanol, acetone, chloroform, hexane). The antioxidant activities of the extracts at different concentrations (10-80 μg/ml) were determined by Copper (II) reduction (CUPRAC), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) and DPPH (1,1-Diphenyl 2-picryl hydrazil) methods. The results were evaluated by comparing them with BHT, BHA and Trolox standard antioxidant substances. It was determined that the antioxidant activities of the extracts of the plant obtained with different solvents were lower than the standard antioxidant substances. However, water extract in the CUPRAC and DPPH methods and ethanol extract in ABTS method showed higher antioxidant activity than other extracts. These results demonstrate that flower extracts of Helichrysum plicatum are available natural antioxidant sources for a variety of applications in the cosmetic, pharmaceutical and food industries.

Keywords:

Helichrysum plicatum, Antioxidant activitiy, CUPRAC, ABTS, DPPH,

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Acet T., Ozcan K., Zengin G. An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum. Journal of food biochemistry 2020; 44(2): e13128.
Akbaş P., Kol ÖG., Gürbüz A., Manap S. Anti-microbial and Anti-oxidant Activity of Watermelon (Citrullus lanatus) Fruit and Watermelon Seed. Celal Bayar Üniversitesi Fen Bilimleri Dergisi 2017; 13(1):139-147.
Albayrak S., Aksoy A., Sagdic O., Hamzaoglu E. Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food chemistry 2010; 119(1): 114-122.
Apak R., Güçlü K., Özyürek M., Karademir SE. Novel total antioxidant activity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. International Journal of Food Science and Nutrition 2004; 52 (26): 7970-7981.
Arıduru R., Arabacı G. Ciğertaze otu (Salvia officinalis) bitkisinin antioksidan aktivitesinin belirlenmesi. Sakarya University Journal of Science 2013; 17(2): 241-246.
Arslan L., Kaya, E. Investigation of antimicrobial and antioxidant activities of Paliurus spina-christi mill. in Kahramanmaras, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 2021; 24(6): 1161-1169.
Aslan M., Orhan Deliorman D., Orhan N., Sezik E., Yesilada E. In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of ethnopharmacology 2007; 109(1): 54-59.
Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants 2015; 4 (3): 6p
Bigovic D., Brankovic S., Kitic D., Radenkovic M., Jankovic T., Savikin K., Zivanovic S. Relaxant effect of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated rat ileum contractions. Molecules 2010; 15(5): 3391-3401.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958, 181 (4617): 1199-1200.
Davis PH., Mill RR., Tan K. Flora of Turkey and East Aeagen Islands. v. X. Edinburgh: Edinburgh University Press, 1988; p. 159-160.
Demir A., Mercanoglu Taban B., Aslan M., Yesilada E., Aykut Aytac S. Antimicrobial effect of Helichrysum plicatum subsp. plicatum. Pharmaceutical Biology 2009; 47(4): 289-297.
Erik S., Güner A., Özhatay N., Ekim T., Başer KHC. Helichrysum Gaertner. Flora of Turkey and East Aeagen Islands 2000; 11, 153-154.
Gülçin İ. Antioxidant and antiradical activities of Lcarnitine. Life sciences 2006; 78(8): 803-811.
Gülçin İ. The antioxidant and radical scavenging activities of black pepper (Piper nigrum) seeds. International Journal of Food Sciences and Nutrition 2005; 56 (7): 491-499.
Jayaprakasha GK., Rao LJ. Phenolic constituents from the lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity. Zeitschrift für Naturforschung C. 2000; 55(11-12):1018-1022.
Meyer JJM., Afolayan AJ., Taylor MB., Erasmus D. Antiviral activity of galangin isolated from the aerial parts of Helichrysum aureonitens. Journal of ethnopharmacology 1997; 56(2): 165-169.
Min DB. Lipid oxidation of edible oils. Food Lipids Chemistry, Nutrition, and Biotechnology, Marcel Dekker, New York, 1998; 283–296 p.
Ozbek T., Gulluce M., Adiguzel A., Ozkan H., Sahin F., Orhan F. Antimutagenic Activity of the Methanol Extract of Helichrysum plicatum ssp. plicatum. Asian Journal of Chemistry 2009; 21(4): 2705-2710.
Panovska TK., Kulevanova S. Antioxidant potential of Helichrysum plicatum DC.(Asteraceae). Instabilities of proteins: theoretical aspects, degradation products and methods for their detection, Macedonian pharmaceutical bulletin 2005; 51(1,2): 29-34.
Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology and Medicine 1999; 26 (9-10):1231−1237.
Schinella GR., Tournier HA., Prieto JM., De Buschiazzo PM., Rıos JL. Antioxidant activity of anti-inflammatory plant extracts. Life sciences 2002; 70(9): 1023-1033.
Süzgeç Selçuk S., Birteksöz AS. Flavonoids of Helichrysum chasmolycicum and its antioxidant and antimicrobial activities. South African Journal of Botany 2011; 77(1): 170-174.
Taşkın T., Gezmiş T., Çam ME., Taşkın D., Çelik BÖ., Şenkardeş İ., Süzgeç Selçuk S. The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum. Brazilian Journal of Pharmaceutical Sciences 2020; 56.
Tepe B., Sokmen M., Akpulat HA., Sokmen A. In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey. Food Chemistry 2005; 90(4): 685-689.
Ünal EL., Mavi A., Kara AA., Çakir A., Şengul M., Yıldırım A. Antimicrobial and antioxidant activities of some plants used as remedies inTurkish medicine, Pharmaceutical Biology 2008; 46, 207-224.
Vujić B., Vidaković V., Jadranin M., Novaković I., Trifunović S., Tešević V., Mandić B. Composition, antioxidant potential, and antimicrobial activity of Helichrysum plicatum DC. various extracts. Plants 2020; 9(3): 337.