Ender ŞİMŞEK, Ayşe Filiz YAVUZ, Emine TERZİ, Tuğba Kevser UYSAL, Beyza Ecem ÖZ BEDİR, Emin HAQVERDİYEV, Özen ÖZENSOY GÜLER

Gebelikte Enzimler: Sitozolik Karbonik Anhidraz, Katalaz, Paraoksonaz 1 ve Ksantin Oksidaz Düzeyleri

Gebelik, birçok vücut fonksiyonu için yüksek enerji ve dolayısıyla daha fazla oksijen gerektiren fizyolojik bir durumdur. Oksijen ve enerji gereksinimlerindeki bu artış, oksidatif streste artışa neden olmaktadır. Gebeliğin 1., 2. ve 3. trimesterlarında yüksek enerji ve artmış oksijen ihtiyacı duyulur. Bu biyokimyasal değişiklikler ile bazı enzim aktiviteleri arasında anlamlı ilişki olabilir. Yapılan bu çalışmada gebelik sırasında sitozolik CA, CAT, PON 1 ve XO enzim aktiviteleri araştırılmıştır. Antioksidan enzimlerin aktiviteleri Biotek cihazı ile spektrofotometrik olarak ölçülmüştür. Sitozolik CA aktivitesi, Wilbur ve Anderson yöntemine göre CO2'nin hidratasyonu ile ölçülmüştür. Bulgulara göre, gebelikte CAT (p=0,048) ve sitozolik CA (p<0,001) enzim aktiviteleri azalırken, PON 1 (p<0,001) ve XO (p=0,016) enzim aktiviteleri artmıştır. Bununla birlikte, sitozolik CA, CAT, PON 1 veya XO enzim düzeyleri açısından 1., 2. ve 3. trimesterlar arasında istatistiksel olarak anlamlı bir fark saptanamamıştır (p>0,05). Farklı antioksidan enzim aktiviteleri hamilelikte artabilir veya azalabilir. Ayrıca, gebe grubunda sitozolik CA enzim düzeyleri azalmasının fetal ve maternal sağlığın biyokimyasal ve fizyolojik yönleri üzerinde önemli etkisi olabilir.

Anahtar Kelimeler:

Gebelik, katalaz, paraoksonaz, ksantin oksidaz, sitozolik karbonik anhidraz

Enzymes During Pregnancy: Cytosolic Carbonic Anhydrase, Catalase, Paraoxonase 1 and Xanthine Oxidase Levels

Pregnancy is a physiological condition which requires high energy and therefore more oxygen for many body functions. This increase in oxygen and energy requirements leads to an increase in oxidative stress. High energy and increased oxygen are needed in the duration of pregnancy - 1st, 2nd and 3rd trimesters. There can be significant correlations between biochemical changes and some enzyme activities. This study investigates the activities of cytosolic CA, CAT, PON 1 and XO enzymes during pregnancy. The antioxidant enzymes’ activities were measured spectrophotometrically using the UV assay method on a Biotek. Cytosolic CA ctivity was measured by the hydration of CO2 in accordance with Wilbur and Anderson's method. According to the findings of this study, CAT (p=0.048) and cytosolic CA (p<0.001) activities decreased during pregnancy whereas both PON 1 (p<0.001) and XO (p=0.016) activity levels were higher in pregnancy. However, there were no statistically significant difference between 1st, 2nd and 3rd trimesters of pregnancy in terms of cytosolic CA, CAT, PON 1 or XO enzyme levels (p>0.05).Different antioxidant enzymes' activities may increase or decrease during pregnancy. The decrease of CA enzyme levels in the group consisting pregnants may have significant impact on biochemical and physiological aspect of fetal and maternal health.

Keywords:

Pregnancy, catalase, paraoxanase, xanthine oxidase, cytosolic carbonic anhydrase,

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