Sayit ALTIKAT, Mehmet Emin BÜYÜKOKUROĞLU, Mehemt ÇİFTÇİ, Nuri BAKAN, ÖMER İRFAN KÜFREVİOĞLU

Kalsiyum glukonat, potasyum klorür ve sodyum bikarbonat'ın insan eritrosit glukoz 6-fosfat dehidrogenaz enzimi üzerine in vitro etkileri

Tedavide sıklıkla kullanılan bazı elektrolitlerin insan eritrosit glukoz 6-fosfat dehidrogenaz enzimi (G6PD) üzerine olan etkileri in vitro koşullarda araştırıldı. G6PD enzimi afinite kromatografisi ile saflaştınldı. Enzim aktivitesinin tayini Beutler metoduna göre spektrofotometrik olarak 340 nm'de yapıldı. Çalışmanın sonuçlarına göre enzim aktivitesini %50 inhibe eden ilaç konsantrasyon (I50) değerleri kalsiyum glukonat için 19,4 mM, sodyum bikarbonat için 160 mM oldu. Potasyum klorür ise G6PD enzimi üzerinde herhangi bir inhibisyon oluşturmadı. Elde edilen verilere göre sodyum bikarbonat daha güçlü olmakla birlikte kalsiyum glukonat da G6PD enziminin aktivitesini iri vitro olarak inhibe etmiştir. Buna karşın potasyum klorürün herhangi bir etkisi görülmemiştir.

Anahtar Kelimeler:

Eritrositler, Glukozfosfat dehidrogenaz, Potasyum klorür, Sodyum bikarbonat, Kalsiyum glukonat

In vitro effects of calcium gluconate, potassium chloride and sodium bicarbonate on human erythrocyte glucose 6-phosphate dehydrogenase

Effects of some electrolytes which are frequently used for treatment, on erythrocyle glucose 6-phosphate dehydrogenase enzyme (G6PD) activity were investigated in in vitro conditions. G6PD was purified by affinity chromatography. Enzyme activity was determined according to the Beutler method by using a spectrophotonieter at 340 nrn. According to the results of study, 50% inhibitor drug concentration values (I50) were 19,4 mM for calcium gluconate and 160 mM for sodium bicarbonate in in vitro study. There was not observed any inhibitor effect of potassium chloride on glucose 6-phosphate dehydrogenase activity. The results of this study revealed that calcium gluconate and sodium bicarbonate have significant inhibition effect but no potassium chloride on the activity of G6PD enzyme in in vitro.

Keywords:

Erythrocytes, Glucosephosphate Dehydrogenase, Potassium Chloride, Sodium Bicarbonate, Calcium Gluconate,

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1. Sodeman WA Jr, Sodeman TM. Sodeman's pathologic physiology mechanism of disease, 1985. Çev: kurul, Fizyopatoloji cilt 2. Ankara, Türkiye Klinikleri Yayınevi, 1991: 720-724. 2. Litz CE, Me Clure JS, Burning RD. Blood and bone marrow. In: Damjanov I, Under J (eds). Anderson's pathology. Missouri, Mosby-Year Book Inc, 1996; 1063-1114. 3. Weksler BB, Moore A, Tepler J. Hematology. In: Andreoli TE, Carpenter CCJ, Plum F, Smith LH Jr (eds). Cecil essentials of medicine. USA, WB Saunders Co., 1990; 342-403. 4. Fairbanks VF, Klee GG. Biochemical aspects of hematology. In: Burtis CA, Ashwood ER (eds). Tietz textbook of clinical chemistry. USA, WB Saunders Co., 1994; 1974-1979. 5. Telefoncu A, Telefoncu F. Glukoz-6-fosfat dehidro- genaz akfivitesine primaquine'nin etkisi. Tr J Medical Sci 1989: 14: 57-63. 6. Berkow R (Editor-in-Chief). The merck manuel, 1992. Çeviri ed: Keklikoğlu M, Tuzcu M. Tanı/ tedavi el kitabı. İstanbul, Nobel Tip Kitabevleri ltd., 1995; 1136-1174. 7. Andrews MM, Mooııey KH. Alterations in hematologic function in children. In: Me Cance KL, Hu ether SE eds. Pathophysiology. Missouri, Mosby-Year Book Inc 1994; 908-942 8. Kayaalp SO. Rasyonel yedavi yönünden tıbbi farmakoloji. Ankara, Feryal Matbaacılık 1998; 153-154. 9. Beuller E. G6PD deficiency. Blood 1994 81:3613-3636. 10. Rang HP, Dale MM, Ritter JM. Pharmacology China. Chürchil Livingstone 1999: 746-756. 11. Shreve DS, Levy HR: On the molecular weight o human glucose-6-phosphate dehydrogenase Biochem Biophys Res Com 1977; 78: 1369-1375. 12. Çiftçi M, Küfrevioğlu Öİ, Gündoğdu M, et al. Effect, of some antibiotics on enzyme activity c glucose-6-phosphate dehydrogenase from humai erythrocytes. Pharmacol Res 2000: 41: 109-113. 13. Ninfali P, Orsenigo T. Barociani L, et al. Rapiı purification of glucose-6-phosphate dehydrogenas from mammal's erythrocyte. Prep Biochem 199C 20: 297-309. 14. Delgado C, Tejedor C, Luquue J. Partial puriiicatio of glucose-6-phosphate dehydrogenase from n: eıythrocyte haenıolysate by partitioning in aqueou two-phase system. J Chromatogr 1990; 49c: 159-168. 15. Morelli A, Benatti U, Gaetani GF, et al. Biochemic; mechanisms of glucose-6-phosphate dehydrogenas deficiency. Proc Natl Acad Sci 1978; 75: 1979-198c 16. Laemmli DK: Clevage of structural proteins durii in assembly of the head of bacteriophage T4. Nairn 1970; 227: 680-83. 17. Beutler E: Red cell metabolism manuel _____ biochemical methods. London, Academic Pres 1971. 18. Tang TK. Free radical theory of erythrocyte aging. Formos Med Assoc 1997; 96(10): 779-783.

19. Tsai KJ, Shih LY, Hung SW, el al. Enhaııcı vesiculation exacerbates complement-depend e: hemolysis in glucose-6-phosphate dehydrogena: deficient red blood cells. Life Sci 1996; 59: 867-871 20. Devi CV, Anandaraj MP. Calcium activated neutr protease & calcium ATPase in glucose-6-phospha dehydrogenase deficiency hemizygotes. Indian Med Res 1995; 101: 39-41. 21. Dememle G, Guida L, Sdraffa A. et al. Mechanisı of perturbation of eıythrocyte calcium homeostaf in lavism. Ceel Calcium 1992; 13: 649-658.