İçme sularındaki kloroformun kuvars kristal mikroterazi sensör ile kolay tespiti

Bu çalışmada, şebeke suyunda fazla bulunması halinde insan sağlığına zararlı olan kloroformun Kuvars Kristal Mikroterazi (QCM) sensör ile kolay tespiti için iki farklı yüzey modifikasyonu yöntemi denenmiştir. İlk kaplama yönteminde, 3-Merkaptopropiltrimetoksisilan’ın (MPS) etil alkol içerisinde sırasıyla asidik ve bazik solüsyonları uygulanmıştır. İkinci kaplama yönteminde ise; ilk aşamada asidik MPS uygulanmış ve daha sonra 2-Merkaptoetanol (ME) kullanılarak yüzey modifikasyonu tamamlanmıştır. Kaplama-1’de, asidik MPS, bazik MPS ve 10 ng/mL kloroform için sırasıyla -180(±30) Hz, -200(±40) Hz ve +40(±10) Hz ortalama frekans kaymaları kaydedilmiştir. Kaplama-2’de, kloroform molekülleri tiyol grupları ile kovalent bağlar oluşturmuş ve asidik MPS, ME ve 10 ng/mL kloroform için elde edilen ortalama frekans kaymaları sırasıyla -180(±30) Hz, -80(±15) Hz ve -70(±8) Hz olmuştur. Ampirik sonuçlara göre, kaplama-2 yöntemi, kaplama-1 yönteminden daha iyi bir yüzey modifikasyonu sunmuştur. MPS + ME ile modifiye edilmiş QCM sensör kullanarak, 0.1, 1, 10 ve 100 ng/mL kloroform konsantrasyonlarında, sırasıyla -2.5(±1), -12(±4), -70(±8) ve -356(±87) Hz rezonans frekansı kaymaları elde edilmiştir.

Anahtar Kelimeler:

Kloroform, Kuvars Kristal Mikroterazi, Yüzey Modifikasyonu, İçme Suyu, Biyosensör

Easy detection of chloroform in drinking water by quartz crystal microbalance sensor

In this study, two different surface modification methods have been tested for easy detection of chloroform, which is harmful to human health in case of excess in the mains water, with a Quartz Crystal Microbalance (QCM) sensor. In the first coating method, acidic and basic solutions of 3-mercaptopropyltrimethoxysilane (MPS) in ethyl alcohol were applied respectively. In the second coating method; Acidic MPS was applied in the first step and then surface modification was completed using 2-mercaptoethanol (ME). In Coating-1, average frequency shifts of -180(±30) Hz, -200(±40) Hz and +40(±10) Hz were recorded for acidic MPS, basic MPS and 10 ng mL chloroform, respectively. In Coating-2, chloroform molecules formed covalent bonds with thiol groups and the mean frequency shifts obtained for acidic MPS, ME and 10 ng / mL chloroform were -180(±30) Hz, -80(±15) Hz, and -70(± 8) Hz, respectively. According to empirical results, coating-2 method provided a better surface modification than coating-1 method. Resonance frequency shifts of -2.5(±1), -12(±4), -70(±8) and -356(±87) Hz respectively were obtained at chloroform concentrations of 0.1, 1, 10, and 100 ng/mL by using QCM sensor modified with MPS + ME.

Keywords:

Chloroform, Quartz Crystal Microbalance, Surface Modification, Drinking Water, Biosensor,

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