Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson

In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma (Roxb.) T. Anderson in MS medium (Murashige and Skoog) including different combinations of Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA). Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA (14.72 shoots/explant). The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm). Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.

Keywords:

In vitro propagation, shoot regeneration, nodal explant tissue culture,

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Abdalla, I. B., Ali, M. A. A. A., Ali, O. A., & Elhassan, A. A. (2021). Effect of cytokinins and auxins on micropropagation of shoot tip and nodal explants of two cultivars of sweet potato (Ipomoea batatas (L) Lam). Gezira Journal of Agricultural Science, 1(2), 84-93.
Amirova, A., Dossymbetova, S., Rysbayeva, Y., Usenbekov, B., Tolegen, A., & Ydyrys, A. (2022). Multiple plant regeneration from embryogenic calli of Paulownia tomentosa (Thunb.) Steud. Plants, 11(8), 1020. https://doi.org/10.3390/plants11081020.
Basha, K. A., Mohamed, S. S., Basha, M. H. G., & Khan, M. K. S. (2022). In vitro regeneration of shoot and roots of the wild folkloric medicinal plant Ammannia baccifera L. via indirect organogenesis from leaf explant cultures. Research Journal of Biotechnology, 17(3), 48-54.
Boopathi, N. M., Harshith, J. D., Santhanakrishnan, V. P., & Raveendran, M. (2021). Tissue Culture and Genetic Engineering in Moringa. In The Moringa Genome (pp. 67-83). Springer, Cham.
Dogan, M. (2017). Multiple shoot regeneration from shoot tip and nodal explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany, 1(1), 4-8. https://doi.org/10.30616/ajb.343304.
Dogan, M. (2018). In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır University Journal of the Institute of Science and Technology, 8, 77-84.
Dogan, M. (2020). Effect of salt stress on in vitro organogenesis from nodal explant of Limnophila aromatica (Lamk.) Merr. and Bacopa monnieri (L.) Wettst. and their physio-morphological and biochemical responses. Physiology and Molecular Biology of Plants, 26(4), 803-816. https://doi.org/10.1007/s12298-020-00798-y.
Dogan, M., & Emsen, B. (2018). Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian Journal of Biological and Chemical Sciences, 1(2), 48-53.
El-Sherif, N. A. (2018). Impact of plant tissue culture on agricultural sustainability. Sustainability of Agricultural Environment in Egypt, Part II, 93-107. https://doi.org/10.1007/698_2017_160.
Eshghi Khas, M., Abbasifar, A., & ValizadehKaji, B. (2020). Optimization of in vitro propagation of purple passion fruit (Passiflora edulis), an important medicinal and ornamental plant. International Journal of Horticultural Science and Technology, 7(3), 305-314. https://doi.org/10.22059/ijhst.2020.297194.342
Hasnu, S., & Tanti, B. (2022). Multiple shoot induction and regeneration of Vanilla borneensis Rolfe-a critically endangered orchid of Assam, India. Plant Science Today, 9(1), 96-104. https://doi.org/10.14719/pst.1292.
Kaya, Y., Arvas, Y. E., & Latifian, E. (2018). Tagetes minuta bitkisinin mikro üretimi üzerine farklı BAP ve IAA konsantrasyonlarının etkileri. International Journal of Life Sciences and Biotechnology, 1(2), 96-104. https://doi.org/10.38001/ijlsb.487354.
Khan, A., Shah, A. H., & Ali, N. (2021). In-vitro propagation and phytochemical profiling of a highly medicinal and endemic plant species of the Himalayan region (Saussurea costus). Scientific Reports, 11(1), 1-13. https://doi.org/10.1038/s41598-021-03032-1.
Lakshmi, P. C., Reddy, C. N., & Srinivas, B. (2021). Effects of bavistin and cefotaxime on in vitro contaminant free shoot regeneration of Ruellia tuberosa L. Plant Tissue Culture and Biotechnology, 31(1), 1-12. https://doi.org/10.3329/ptcb.v31i1.54106.
Majumder, S., & Rahman, M. M. (2016). Micropropagation of Stevia rebaudiana Bertoni. through direct and indirect organogenesis. Journal of Innovations in Pharmaceutical and Biological Sciences, 3, 47-56.
Malothu, G. S., Marka, R., & Nanna, R. S. (2022). High frequency callus mediated plantlet regeneration from different explants of ethno-medicinal plant Turkey berry (Solanum torvum Sw). Journal of Scientific Research, 66(1), 121-128.
Mangena, P. (2021). Analysis of correlation between seed vigour, germination and multiple shoot induction in soybean (Glycine max L. Merr.). Heliyon, 7(9), e07913. https://doi.org/10.1016/j.heliyon.2021.e07913.
Murashige, T., & Skoog F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiologia Plantarum, 15, 473-497.
Naik, P. S., & Buckseth, T. (2018). Recent advances in virus elimination and tissue culture for quality potato seed production. Biotechnologies of Crop Improvement, 1, 131-158. https://doi.org/10.1007/978-3-319-78283-6_4.
Nayak, P., & Kalidass, C. (2016). In vitro regeneration of Blepharispermum subsessile DC: an endangered medicinal plant of Odisha, India using cotyledon explants. Plant Tissue Culture and Biotechnology, 26(2), 255-266. https://doi.org/10.3329/ptcb.v26i2.30575.
Sasidharan, P., & Jayachitra, A. (2017). Direct shoot bud regeneration from shoot tip explants of Enicostema axillare: an important medicinal plant. Agroforestry Systems, 91(3), 471-477. https://doi.org/10.1007/s10457-016-9943-x.
Sirangi, S., Jogam, P., Rohela, G. K., Ragan, A., & Raju, V. S. (2022). Micropropagation of endemic Corynandra chelidonii var. pallae (Cleomaceae) through nodal explants and validation of their genetic integrity by ISSR markers. Vegetos, 35(2), 511-519. https://doi.org/10.1007/s42535-021-00302-1.
Tiku, A. R., Thomas, T. D., & Razdan, M. K. (2021). Plant Tissue Culture in Mulberry Improvement. In Mulberry: Genetic Improvement in Context of Climate Change (pp. 108-121). CRC Press.
Yun, A., Kim, T. D., Kim, J. A., Lee, N. N., Cheong, E. J., & Kim, Y. W. (2022). Plant regeneration through multiple-shoot induction and ex vitro rooting in Vaccinium oldhamii Miq. Journal of Plant Biotechnology, 49(1), 82-89. https://doi.org/10.5010/JPB.2022.49.1.082.