VARİKÖZ VEN HASTALARINDA DOKU ESER ELEMENT DÜZEYLERİNİN ATOMİK ABSORBSİYON SPEKTROMETRESİ İLE TAYİNİ

Amaç:Günümüzde venöz yetmezlik hala ciddi bir klinik problemdir. Etiyolojisinin kesin nedeni ve moleküler mekanizmaları hala bilinmemekle beraber mekanik ve intrinsik faktörlerin varlığı ileri sürülmektedir. Bu çalışmada, alt ekstremite varisleri olan hastaların ven dokularında, antioksidan enzimlerin yapısında kofaktör olarak görev yaparak, organizmanın oksidan/antioksidan dengesine katkı sağlayan eser elementlerden Zn, Cu ve Fe’in düzeylerini inceledik. Elde ettiğimiz sonuçlar ile elementlerin düzeyindeki değişikliklerin venöz yetmezliğin gelişimi ve/ ve ya ilerlemesinde oynadığı rolü açıklamayaçalıştık. Materyal ve Metot:Çalışmamız alt ekstremitede primer varis tanısı nedeniyle opere edilecek 40 kişi ve koroner arter bypass greft operasyonu geçirecek 40 kişinin vena safena magna dokuları üzerinde gerçekleştirildi. Alınan ven doku örneklerinde eser elementlerden Zn, Cu ve Fe ölçümleri atomik absorbsiyon spektrometresi ileyapıldı. Bulgular:Kontrol grubu ile karşılaştırıldığında Zn düzeyleri çalışma grubunda istatistiksel olarak anlamı seviyede düşük, Fe düzeyleri ise anlamlı olarak yüksek saptanmıştır. Cu düzeyleri çalışma grubunda kontrol grubuna kıyasla yüksek olmasına rağmen, bu fark anlamlı değildir. Çalışma grubunda, eser elementler açısından herhangi bir korelasyon gözlenmemiştir. Sonuç:Variköz ven doku örneklerinde düşük saptanan Zn düzeylerinin azalmış antioksidan aktivite ile ilişkili olduğu düşünülmüştür. Artan Cu düzeyi ise, artmış kollajen ve elastin yapımı nedeni ile gerçekleşmiş olabilir. Çalışma grubunda dokularda artmış Fe birikiminin, hasarlı dokuya çökerek oksidatif stresi tetiklediğini de düşünmekteyiz.

Determination of Tissue Trace Element Levels with Atomic Absorption Spectrometry in Varicose Vein Patients

Aim:Currently, venous insufficiency is still a serious clinical problem. The exact cause and molecular mechanisms of the etiology are still unknown, but the presence of mechanical and intrinsic factors has been suggested. In this study, we excamined (Zn, Cu and Fe) levels of trace elements contributing to the oxidant / antioxidant balance of the organism by acting as a cofactor in the structure of antioxidant enzymes in the veins of patients with lower extremity varices. With the results we obtained, we tried to explain the changes in the level of the elements in the development and / or progress of venousinsufficiency. Materials and Methods:Our study was carried out on 40 patients who underwent surgery for the diagnosis of primary varicose veins in the lower extremity and 40 patients who underwent coronary artery bypass graft surgery and on their vena safena magna tissues. Levels of Zn, Cu and Fe were measured with atomic absorption spectrometry in vein tissue samples. Results:Zn levels were significantly lower in the study group whereas Fe levels were significantly higher in the study group compared to the control group. Although Cu levels were higher in the study group compared to the control group, this difference was not significant. In the study group, no correlation was observed in terms of trace elementlevels. Conclusions:Low levels of Zn in tissue samples were thought to be associated with decreased antioxidant activity. Increased Cu level may be due to increased collagen and elastin production. We assume that increased Fe accumulation in tissues in the study group was triggered oxidative stress by adsorbing on the damaged tissue.

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