Cansu DİNDAR, Adnan YILDIRIM, Civan ÇELİK

In vitro koşullarda farklı dozlarda bor uygulamalarının mersin (Myrtus communis L.) bitkisinin biyokimyasal özellikleri üzerine etkileri

Araştırmada siyah meyveli bir mahalli çeşit olan ‘tatlı mersin’ genotipinin sürgün uçları materyal olarak kullanılmıştır. Bu çalışma ile ortama eklenen bor konsantrasyonlarından toksisite problemi yaratabilecek bor (B) uygulamaları ile olası B stresi altında mersin bitkisinin morfolojik ve biyokimyasal özelliklerdeki değişimleri incelenmiştir. Bu amaçla 6 farklı borik asit (H3BO3) dozu (12.4 mg L-1, 18.6 mg L-1, 24.8 mg L-1, 31 mg L-1, 37.2 mg L-1, 43.4 mg L-1) Murashige ve Skoog (MS) ortamına eklenmiş ve kontrol grubu ile birlikte bitkilerin gelişimleri takip edilmiştir. Araştırmada kontrol ortamındaki bitkilere yalnızca MS ortam içeriğindeki standart B miktarı (6.2 mg L-1) ilave edilmiştir. Araştırmada, en yüksek prolin miktarı 43.4 mg L-1 uygulamasında 11.6 µg ml-1 olarak elde edilirken, en düşük prolin miktarı kontrol grubunda 3.3 µg ml-1 olarak belirlenmiştir. Askorbat peroksidaz (APX), süperoksit dismutaz (SOD), peroksidaz (POD) enzim aktiviteleri uygulama dozları arttıkça yükselmiş, en düşük değer kontrol grubundaki bitkilerde belirlenmiştir. Sonuç olarak; yetiştirme ortamına eklenen H3BO3 miktarı arttıkça özellikle askorbat peroksidaz, katalaz, süperoksit dismutaz ve peroksidaz gibi stres mekanizmasında rol alan enzimatik antioksidan aktivitelerinde önemli derecede artış gözlenmiştir.

Anahtar Kelimeler:

Mikroçoğaltım, Bor toksisitesi, Mersin bitkisi, Myrtus communis L., In vitro, Stres

The effects of boron applications with different doses on the biochemical properties of myrtle plant (Myrtus communis L.) in in vitro conditions

In this study, shoot tips of the 'sweet myrtle' genotype which is a local variety with black fruits, were used as study material. It was aimed to determine the level of boron (B) that may cause toxicity problems from six different B concentrations added to the growing media and the changes in biochemical properties of myrtle plants under possible boron stress were examined. Six different boric acid (H3BO3) doses (12.4 mg L-1, 18.6 mg L-1, 24.8 mg L-1, 31 mg L-1, 37.2 mg L-1, 43.4 mg L-1) was added to the Murashige and Skoog (MS) medium and the development of the plants was compared with the control group. Only standard B content (6.2 mg L-1) was added in the MS medium to the plants in the control group. As a result of examining the statistical evaluations; the highest proline amount was obtained as 11.6 µg ml-1 in 43.4 mg L-1 application in the media which has the highest H3BO3 concentration, whereas the lowest proline amount was determined as 3.3 µg ml-1 in the control group. Ascorbat peroxidase, superoxide dismutase, peroxidase enzyme activities increased as the application doses increased and the lowest value was determined in the plants in the control group. As the amount of B added to the growing medium increased, a significant increase was observed in the enzymatic antioxidant activities that play a role in the stress mechanism, especially ascorbat peroxidase, catalase, superoxide dismutase, peroxidase. As a result of the study, it was determined that the tolerance limits of the myrtle plant against boron stress were high.

Keywords:

Boron toxicity, Myrtle, Myrtus communis L., In vitro, Stress, Micropropagation,

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