Şener KURT, Soner SOYLU, Aysun UYSAL, E. Mine SOYLU, Merve KARA

Ceviz gövde kanseri hastalığı etmeni Botryosphaeria dothidea’nın tanılanması ve bazı fungisitlerin hastalık etmenine karşı in vitro antifungal etkinliklerinin belirlenmesi

Amaç: Çalışmada Hatay’ın İskenderun ilçesinde Mayıs 2019 tarihinde ceviz bahçelerdeki ceviz ağaçlarında, iletim dokularında kahverengi-koyu kahverengi renk değişikliği, odun dokusu nekrozu ve dallarda geriye doğru ölüm hastalık belirtilerine neden olan fungal etmenin tanılanması ve etmenin bazı fungisitlere karşı duyarlılık düzeylerinin belirlenmesi amaçlanmıştır. Yöntem ve Bulgular: Hastalıklı dallardan, kanserlerden ve belirti göstermeyen ceviz örneklerinden yapılan izolasyonlar sonucunda elde edilen fungal izolat morfolojik ve moleküler çalışmalara göre Botryosphaeria dothidea olarak tanılanmıştır. Patojenisite denemeleri, fungal izolatın bölgede yetiştirilen Chandler çeşidi ceviz ağacı sürgünlerinde oldukça virülent olduğunu göstermiştir. Mevcut bilgilerimize göre bu çalışma, Türkiye’de cevizi enfekte eden Botryosphaeria dothidea’nın ilk raporudur. Fluazinam (0.001-0.015 µg/ml), thiophanate-methyl (0.1-10.0 µg/ml), boscalid + pyraclostrobin (1.0-50.0 µg/ml), trifloxystrobin (0.5-50.0 µg/ml), kresoxim-methyl (0.1-50.0 µg/ml) ve tebuconazole (0.5-50.0 µg/ml) gibi 6 farklı fungisitin farklı konsantrasyonlarının, fungal etmene karşı in vitro antifungal etkileri belirlenmiştir. Fungisitlerin ortalama etkili konsantrasyon (EC50) değerleri, Probit analiziyle belirlenmiştir. Hesaplanan EC50 değerlerine göre hastalık etmenine karşı en etkili fungisit fluazinam (0.002 µg/ml) olarak kaydedilmiş olup, bu fungisiti thiophanate-methyl (0.275 µg/ml), tebuconazole (0.994 µg/ml), boscalid + pyraclostrobin (3.993 µg/ml) ve trifloxystrobin (4.262 µg/ml) izlemiştir. En düşük antifungal etkinlik fungisitler arasında misel gelişimini %37.5 oranında engelleyen kresoxim-methyl tarafından gösterilmiştir. Genel Yorum: Bu çalışma fungal etmen Botryosphaeria dothidea’nın Türkiye’de yetiştirilen ceviz ağaçlarında gövde kanseri hastalığa neden olduğunun ilk kayıdıdır. Elde edilen sonuçlar ayrıca Botryosphaeria dothidea’nın sebep olduğu ceviz kanser hastalığının seçilen fungisitlerin uygulanmasıyla ağaçların hastalıktan korunabileceğini önermektedir. Çalışmanın Önemi ve Etkisi: Sonuç olarak, fluazinam, thiophanate-methyl ve tebuconazole’ün fungal patojen B. dothidea’nın kontrolünde kullanım potansiyeli bulunduğunu ortaya koymaktadır.

Identification of walnut stem canker disease agent Botryosphaeria dothidea and determination of in vitro antifungal efficiencies of some fungicides against the disease agent

Aims: Symptoms of canker disease on walnut trees included brown to dark brown discoloration of vascular tissues, wood necrosis, and branch dieback were observed in several orchards in İskenderun City of Hatay Province in May 2018. The aims of this study were to identify the fungal pathogen and to determine its sensitivities against some fungicides. Methods and Results: Isolations were made from diseased branches, cankers and asymptomatic walnut samples and causal disease agent was identified as Botryosphaeria dothidea based on morphological characteristics and molecular techniques. Pathogenicity tests showed that fungal isolate were highly virulent on shoots of walnut (cv. Chandler) trees commonly grown in the region. To our knowledge, this is the first report of Botryosphaeria dothidea infecting walnut in Turkey. In vitro antifungal effect of different concentrations of six fungicides, fluazinam (0.001-0.015 µg/ml), thiophanate-methyl (0.1-10.0), boscalid + pyraclostrobin (1.0-50.0 µg/ml), trifloxystrobin (0.5-50.0 µg/ml), kresoxim-methyl (0.1-50.0), and tebuconazole (0.5-50.0 µg/ml) were also evaluated against the pathogen. The mean effective concentration (EC50) values were determined by Probit analysis. According to EC50 values, fluazinam (0.002 µg/ml) was recorded as the most effective fungicide and it was followed by thiophanate-methyl (0.275 µg/ml), tebuconazole (0.994 µg/ml), boscalid + pyraclostrobin (3.993 µg/ml) and trifloxystrobin (4.262 µg/ml). The lowest antifungal efficacy was followed by kresoxim-methly inhibiting mycelial growth by 37.5% at the highest concentration used.. Conclusions: To our knowledge, this is the first report of Botryosphaeria dothidea causing stem canker diseases on walnut trees grown in Turkey. Our results also suggest that the walnut canker disease caused by Botryosphaeria dothidea could be reduced effectively with the application of selected fungicides. Significance and Impact of the Study: In conclusion, our results suggest that fluazinam, thiophanate-methyl and tebuconazole have the potential effects to control Botryosphaeria dothidea.

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