SAFLAŞTIRILAN MİTOKONDRİAL TİYOREDOKSİN REDÜKTAZ ENZİMİNİN AKTİVİTESİ ÜZERİNE İN VİTRO ETKİLERİNİN İNCELENMESİ

Bu çalışmada, hindi (Meleagris gallopavö) karaciğerinden saflaştırılan mitokondriyal tiyoredoksin redüktaz enzimi aktivitesi üzerine bazı herbisit ve insektisitlerin in vitro etkileri incelendi. Enzimin saflaştırılmasında gradientli santrifügasyonla homojenizasyondan sonra 2', 5'-ADP Sepharose 4B afinite kromatografisi yöntemi kullanıldı. Enzim hindi karaciğer mitokondrisinden 3,07 EÜ/mg protein spesifik aktiviteyle, % 2,05 verimle, ve 990 kat saflaştırıldı. Enzimin saflık kontrolü Sodyum Dodesil Sülfat-Poliakrilamid Jel Elektroforezi (SDS-PAGE) ile yapıldı ve tüm kinetik çalışmalarda saf enzim kullanıldı. Yapılan kinetik çalışmalarda kullanılan 4 herbisit ve 4 insektisitten 3 herbisit ve 3 insektisitin enzim aktivitesi üzerinde inhibisyon etkisi olduğu tespit edildi.

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