The purpose of this study was to determine the effects of dietary addition of multi-enzymes in different source of grains based diets on performance and egg quality characteristics of laying hens. A total of 225, 24 weeks of old laying hens (Atak-S) were divided into 3 treatments with 5 replicates and 15 hens per replicate for 10 weeks. The control group was fed basal diet (without any supplementation) and treatment groups were fed basal diet supplemented 0.1% Enzyme-A (xylanase, ß-glucanase, cellulase, α-amilase and protease) and 0.05 % Enzyme-B (xylanase, ß-glucanase). Performance and egg quality parameters were checked weekly throughout the experiment. Dietary multi-enzymes supplementation significantly changed the shape index, yolk index and yolk color (L and a) at different weeks of trial (P<0.05). However, daily feed intake, egg production, average egg weight and feed conversion rate were not significantly affected by addition of both multi-enzymes during the experimental period (P>0.05). In addition, dietary addition two types of multi-enzymes did not affect the egg specific gravity, eggshell thickness, eggshell rate, albumen index, haugh units (P>0.05). As a result, it was determined that the addition of multi-enzyme additions to the laying hens of the hens between 24-33 weeks of age did not affect the performance parameters,  but caused limited changes on some egg quality characteristics.