NADİRE ESER, Kezban KARTLAŞMIŞ, Yılmaz UÇAR, Umut KÖKBAŞ

Ferula elaeochytris Kök Ekstresinin Toplam Fenolik Bileşikler ve Glukoz Seviyesi Üzerine Etkisi

Günümüzde önemli bir sorun haline gelen diyabet, insülin sekresyonu, insülin etkisi veya her ikisindeki sorunlardan kaynaklanan hiperglisemi ile karakterize bir metabolik hastalıktır. Diyabet hastalığının vasküler ve nörojenik komplikasyonlarının gelişiminde oksidatif hasarın önemli rol oynadığını ve bu patolojiler üzerinde antioksidanların etkili olduğu bilinmektedir. Güney ve Güneydoğu Anadolu bölgesinde sıkça kullanılan “ÇAKŞIR” diye bilinen Ferula elaeochytris kök ekstresinin toplam fenolik içeriği ve glukozu inhibe edici etkisinin araştırılması amaçlanmıştır. Kahramanmaraş Engizek yaylasından toplatılan Ferula elaeochytris bitkisinin kök kısmı temiz ve kuru bir zeminde kurutulduktan sonra öğütülerek, işleme alınmak üzere hazırlanmıştır. Ferula eleaochytris’in glukoz üzerine etkisi biyosensör cihazı ile elektrokimyasal olarak; antioksidan etkinliğinin bir göstergesi olarak da Folin-Ciocalteu yöntemi ile toplam fenolik içeriği tayin edilmiştir. Bu çalışmada, biyosensör cihazı ile elektrokimyasal incelemesinde, Ferula elaeochytris kök ekstresinin 100’er µl kümülatif olarak 1 dakika (dk) aralıkla eklenmesi sonucu enzim-substrat etkileşiminin azalmasına bağlı olarak glukoz seviyeleri üzerinde doğrusal bir düşüş olduğu gözlemlenmiştir (N=120). Ayrıca, bu bitkinin Folin-Ciocalteu yöntemi ile toplam fenolik içeriği 41.45±2.38 mg GAE/g (n:3) olarak tespit edilmiştir. Ferula elaeochytris’in glukoz seviyesini inhibe edici etkisinden dolayı hipoglisemik ve toplam fenolik içeriğinin yüksek kapasiteye sahip olması yönüyle ise antioksidan kaynağına sahip olduğu söylenebilir. Yapılacak olan ileri çalışmalarla, insanlar tarafından bilinçsizce kullanılan bu bitkinin yerinde ve amacına uygun daha bilinçli bir kullanımı sağlanabilir.

Anahtar Kelimeler:

Ferula elaeochytris, biyosensör, fenolik bileşikler, glukoz

Total Fenolic Contents of Ferula elaeochytris Root Extract and Its Effect on Glucose Levels

Nowadays, diabetes is a metabolic disease characterized by hyperglycemia by a problem in insulin secretion, insulin action, or both. It is known that oxidative damage plays an important role in the development of vascular and neurogenic complications of diabetes and antioxidants are effective on these pathologies. The aim of this study was to investigate the possible antidiabetic and antioxidant effects of Ferula elaeochytris root extract, commonly known as “ÇAKŞIR” in South and Southeastern Anatolia. Ferula elaeochytris was collected from the Engizek plateau of Kahramanmaraş. The plant root was prepared on a clean and dry surface and ground for grinding. The inhibitory effect of Ferula eleaochytris on glucose was determined electrochemically with the biosensor device, and total phenolic content was determined by Folin-Ciocalteu method as an indicator of antioxidant activity. In this study, according to electrochemical analysis with biosensor device, a linear decrease in glucose levels was observed depends on the decrease of enzyme-substrate interaction as a result of the addition of 100 µl cumulatively 1 minute (min) intervals of Ferula elaeochytris root extract (N=120). In addition, the total phenolic content of this plant was detected (41.45±2.38 mg GAE/g, n:3) by Folin-Ciocalteu method. It can be concluded that Ferula elaeochytris may have a hypoglycemic effect owing to its inhibitory effect on glucose levels and Ferula elaeochytris may also have a possible antioxidant source by means of the plant has a high capacity of total phenolic content. With the advanced studies to be done, it will be able to provide a more conscious utilization in accordance with the purpose of this unconsciously used plant by many people.

Keywords:

Ferula elaeochytris, biosensor, phenolic compounds, glucose,

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