Bilal KOŞAR, Burak TAN, Melek ALTUNKAYA, Cem SÜER, Nurcan DURSUN

Sıçanlarda 6-n-propil-2-tiourasil ile bozulan öğrenme ve bellek performansına selenyumun etki

Amaç: Bu çalışma ile sıçanlarda 6-n-propil-2-tiourosil (PTU) ile hipotiroidi oluşturularak bozulan uzamsal öğrenme ve bellek performansına selenyumun (Se) iyileştirici etkisini ortaya koymak amaçlanmıştır. Gereç ve Yöntem: Çalışma vücut ağırlıkları 250-300 gr olan, genç erkek Wistar albino sıçanlar üzerinde yapıldı. Sıçanlar kontrol (Kontrol), hipotiroidi (PTU), PTU ile berabler 5 ppm Se verilen (P-5Se) ve PTU ile berabler 10 ppm Se verilen (P-10Se) olarak dört gruba ayrıldı. Sıçanların plazma sT3, sT4 değerleri ELİSA yöntemiyle, hipokampus Se değerleri ise atomik absorpsiyon spektrometresi ile ölçüldü. Öğrenme/bellek performansının değerlendirilmesi için Morris su tankı testi (MST) kullanıldı. Bulgular: Öğrenme testinin ilk gününden dördüncü güne doğru tüm grupların yüzme mesafelerinde ve kaçış sürelerinde anlamlı düzeyde azalma görüldü. Gruplar arası istatistiksel karşılaştırmada yüzme mesafesi uygulamanın birinci gününde hipotiroidili grupta kontrole göre artış, Se verilen hipotiroidili gruplarda azalma bulundu. Hedef kadranda bulunma oranı hipotiroidi ile azalma, 5 ppm Se verilen grupta, Se verilmeyen hipotiroidili gruba göre artış gösterdi. Sonuç: Bu çalışma hipotiroidinin hipokampal öğrenme ve bellek fonksiyonunu olumsuz etkilediğini, selenyum takviyesinin ise hipotirodinin bu olumsuz etkisini düzelttiğini ortaya koymuştur.

Anahtar Kelimeler:

Hipotiroidi, Selenyum, Öğrenme, Bellek, Morris su tankı testi, Hypothyroidism, selenium, learning, memory, morris water maze test

Effect of selenium on impaired learning and memory performance by 6-n-propyl-2-thiouracil in rats

Aim: In this study, it was aimed to reveal that the healing effect of selenium on spatial learning and memory performance impaired by hypothyroidism with 6-n-propyl-2-thiouracil in rats. Method: The study was carried out on young male Wistar albino rats weighing between 250 and 300 gr. The rats were divided into four groups which were control, hypothyroid, 5 ppm selenium supplied with 6-n-propyl-2-thiouracil and 10 ppm selenium supplied with 6-n-propyl-2-thiouracil group. Plasma fT3 and fT4 levels of rats were measured by ELISA method and hippocampus selenium levels were measured by atomic absorption spectrometry. Morris water maze test was used to evaluate learning/memory performance. Results: A significant decrease was observed in distance moved and escape latency of all groups from the first day to the fourth day of the learning test. In the statistical comparison between groups, distance moved showed an increase in the hypothyroid groups than that the control group and decrease in 10 ppm selenium supplemented hypothyroid group compared to hypothyroidism without selenium. The ratio of presence in the target quadrant decreased with hypothyroidism and increased in the group supplemented 5 ppm selenium compared to hypothyroidism without selenium. Conclusion: This study revealed that hypothyroidism may negatively affects hippocampal learning and memory function, and selenium supplementation may improve this negative effect of hypothyroidism.

Keywords:

Hypothyroidism, selenium, learning, memory, morris water maze test,

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