Rahşan YILMAZ, Nihat YUMUŞAK, Hasan İkbal ATILGAN, Gökhan KOCA, Meliha KORKMAZ

Radyoiodin (131I) Uygulanan Ratlarda Karaciğerdeki Histopatolojik Bulgular ve Hepatik Satellate Hücrelerde Artış

Radyoaktif iyot (131I) hipertiroidizm ve tiroid kanserlerinin tanı ve tedavisi amacıyla kullanılan bir radyonükliddir. Bu çalışma ile ratlarda radyoiodinden (131I) kaynaklanan karaciğer hasarının histopatolojik ve immunohistokimyasal olarak değerlendirilmesi amaçlandı. Çalışmada 20 adet dişi Wistar Albino rat kullanıldı ve bunlar rastgele hiçbir işleme tabi tutulmayan kontrol grubu (n=10) ve 131I uygulama grubu (n=10) şeklinde ikiye ayrıldı. Üçüncü ayın sonunda nekropsileri yapılan ratların karaciğer dokuları rutin doku takibine alındı. Karaciğerin histopatolojik incelemesinde 131I grubunda kapsula altında granulom oluşumlarına rastlandı. İmmunohistokimyasal boyamada 131I grubunda hepatik satellate hücrelerin (HPCs), yoğunluğunu gösteren GFAP boyamalarının kontrol grubundan daha yüksek bir değere sahip olduğu görüldü.

Anahtar Kelimeler:

131I, histopatoloji, karaciğer, HPCs, GFAP, immunohistokimya

Hystopathological Findings and Hepatic Satellite Cells İncrease in the Liver of the Rats Applied Radionin (131I)

Radioactive iodine (131I) is a radionuclide used for the diagnosis and treatment of thyroid cancers and hyperthyroidism. This study was aimed to evaluation by histopathological and immunohistochemical of hepatic damage caused by radioiodine (131I) in the rat. Twenty female Wistar Albino rats were used in the study and they were randomly divided into two groups as control group (n = 10) and 131I application group (n = 10). At the end of the third month, the liver tissues were taken of rats at the necropsy, the tissue specimens underwent routine tissue processing. Histopathological examination was observed granuloma formation in the liver in 131I group. Immunohistochemical staining the GFAP was showed in the 131I group the higher than in the control group, that showed density of the hepatic satellite cells (HPCs).

Keywords:

131I histopathology, liver, HPCs, GFAP, immunohistochemistry, rat,

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