Zafer ÖZYILDIZ, Özlem ÖZMEN, Hüseyin DOLU

Buzağılarda Respiratuar Sinsityal Virus (BRSV) Enfeksiyonlarında Patolojik ve İmmunohistokimyasal İncelemeler

Bu çalışmada Burdur İli’ndeki buzağılarda saptanan respiratuar sinsityal virus (BRSV) enfeksiyonları makroskobik, histopatolojik ve immunohistokimyasal bulgularıyla detaylı olarak incelendi. Solunum problemleri ile ölen ve immunohistokimyasal olarak BRSV tespit edilen buzağıların nekropsisinde makroskobik olarak kraniyo-ventral loblarda koyu kırmızı renkli sert kıvamlı pnömoni alanları gözlendi. Bu alanların kesit yüzlerinden köpüklü ve mukopurulent bir eksudat sızıyordu. Mikroskobik olarak akciğer kesitlerinde intersitisyel ve bronko-intersitisyel pnömoni bulguları gözlendi. Pnömoni bölgelerinde yoğun şekilde karakteristik sinsityal hücreler ve bazı bronş-bronşiyol epitellerinde intrasitoplazmik inklüzyon cisimciklerine rastlandı. İmmunohistokimyasal incelemelerde; bronş, bronşiol ve alveol epitelleri ile lümenlerindeki yangısal hücre ve özellikle sinsityal hücrelerin sitoplazmalarında immuno-pozitif alanlara rastlandı. Bu çalışmanın amacı; Burdur bölgesinde buzağılarda ciddi kayıplara neden olan BRSV hastalığında makroskobik, mikroskobik ve immunohistokimyasal bulgularının detaylı olarak sunulmasıdır.

Anahtar Kelimeler:

BRSV, buzağı, patoloji

Pathological and Immunohistochemical Studies in Respiratory Cyncitial Virus (BRSV) Infection in Calves

In this study, bovine respiratory syncytial virus (BRSV) infections of calves were examined in detail with macroscopic, microscopic and immunohistochemical findings in Burdur province. At necropsy of calves that died from respiratory findings and immunohistochemically BRSV diagnosed, dark red and hard areas in the cranio-ventral lobes grossly. Foamy and mucopurulent exudate was leaking from the cross-sections of these areas. Microscopically, findings of interstitial and broncho-interstitial pneumonia were observed in the lung sections. At the pneumonic areas, marked characteristic syncytial cells and intra cytoplasmic inclusion bodies were seen in some bronchiolar and bronchial epithelial cells. Immunohistochemically, immuno-positive areas were seen in the bronchial, bronchiolar and alveolar epithelial cells and the cytoplasm of inflammatory cells especially syncytial cells. The aim of this study was detailed presentation of macroscopical, microscopical and immunohistochemical findings of BRSV disease in calves which cause serious economic losses in Burdur province.

Keywords:

BRSV calf, pathology, immunohistochemistry,

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