Muzaffer DÜKEL, Ayse Gul MUTLU, Umit KEBAPCI, Zeki YILDIRIM

Comparing the Efficacy of mtDNA COI-based Taxonomy in the Freshwater Gastropod Genus Graecoanatolica in West Anatolia (Gastropoda: Hydrobiidae)

It is very important to be knowledgeable in taxonomic identification between species for biological disciplines. The genus Graecoanatolica spread West Anatolia in Turkey. Molecular Systematics of the Graecoanatolica species have not been investigated however this genus morphological characteristics have been comprehensively studied. In this research, phylogenetic analysis of cytochrome oxidase subunit I (620bp) sequences for all living species in Anatolia of Graecoanatolica were performed and phylogenetic trees were created by using COI sequence results. mitochondrial DNA (mtDNA) COI gene sequences of different genus were achieved from GenBank and used as outgroup for molecular systematic analysis. mtDNA COI sequence data analysis suggest that species determined as G. lacustristurca in Burdur is sister G. tenuis in Denizli but it is diffirent species. Also we found out that molecular data shows G. pamphylica is different molecularly from genus Graecoanatolica which has been described in Antalya Kirkgöz spring as Graecoanatolica species.Keywords: Hydrobiidae, Graecoanatolica, COI, phylogeny, taxonomy

PDF

___

Albrecht, C., Lohfink, D. and Schultheiß, R. (2006). Dramatic decline and loss of mollusc diversity in long-lived lakes in Greece. Tentacle 14: 11-13.
Arconada, B. and Ramos M. (2003). The ibero-balearic region: one of the areas of highest Hydrobiidae (Gastropoda, Prosobranchia, Rissooidea) diversity in Europe. Graellsia, 59(2-3): 91-104.
Bieler, R. (1992). Gastropod phylogeny and systematics. − Annual Review of Ecology and Systematics, 23: 311–338.
Davis, J.P., Akella, S. and Waddell, P.H. (2004). Accelerating phylogenetics computing on the desktop: experiments with executing UPGMA in programmable logic. Engineering in Medicine and Biology Society (IEMBS), 4: 2864–2868.
Bodon, M., Cianfanelli, S., Talenti, E., Manganelli, G. and Giusti, E. (1999). Litthabitella chilodia (Westerlund, 1886) in Italy (Gastropoda: Prosobranchia: Hydrobiidae). Hydrobiologia, 411: 175–189.
Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol., 3(5): 294-299.
Georgiev, D. and Glöer, P. (2011). Two new species of a new genus Devetakia gen. n. (Gastropoda: Hydrobiidae) from the caves of Devetashko plateau, North Bulgaria. Acta Zool Bulg, 63(1): 11-16.
Griffith, H.I., Reed, J.M., Leng, M.J., Ryan, S. and Petkovski, S. (2002). The recent palaeoecology and conservation status of Balkan lake Dojran. Biol. Conserv. 104: 35-49.
Hall, T. (1994). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser., 41: 95-98.
Kabat, A. and Hersler, R. (1993). The Prosobranch family Hydrobiidae (Gastropoda: Rissooidea): Review of classification and supraspecific taxa. Smithsonian Institution Press, Washington, 84pp.
Kebapci, U., Koca, S.B. and Yildirim, M.Z. (2012). Revision of Graecoanatolica (Gastropoda : Hydrobiidae ) species in Turkey. Turk J Zool., 36(4): 399-411.
Keeton, W.T., Gould, J.L. and Gould, C.G. (1999). Biological Science. W. W. Norton & Company, London. 490-503pp.
Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol., 16(2): 111–120.
Knight, J., Batten, R., Yochelsen, E. and Cox, L. (1960). Supplement, Paleozoic and some Mesozoic Caenogastropoda and Opisthobranchia. – In: R. C. Moore (Ed.): Treatise on invertebrate paleontology, Part 1, Mollusca 1. Geological Society of America & University of Kansas Press, New York & Lawrence, 310-331pp.
Kumar, S., Nei, M., Dudley, J. and Tamura, K. (2006). MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform, 88(4):559-566. doi:10.1093/bib/bbn017.MEGA.
Koca, S.B. (2007). Batı Anadolu Rıssoacea (Gastropoda, Prosobranchıa) Faunasının Belirlenmesi. PhD Thesis. Isparta; Süleyman Demirel University.
Librado, P. and Rozas, J. (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11):1451-1452.
Ponder, W.F., Clark, S.A. and Miller, A.C. (1999). A new genus and two new species of Hydrobiidae (Mollusca: Gastropoda: Caenogastropoda) from south Western Australia. J R Soc West Aust, 82(3): 109-120.
Radoman, P. (1973). New Classifi cation of Fresh and Brackish Water Prosobranchia from the Balkans and Asia Minor. Posebna I zdanja, Prirodnjacki Muzej u Beogradu, Belgrade.
Radoman, P. and Stankovic, S. (1979). Jedan novi predstavnik roda Graecoanatolica iz Dojranskog jezera. Glasnik Prirodnjatshkog Muzeja u Beogradu, Serija B, Bioloshke Nauke, Belgrade.
Radoman, P. (1983). Hydrobioidea, a Superfamily of Prosobranchia (Gastropoda), I. Systematics. Serbian Academy of Sciences and Arts, Monographs DXLVII, Department of Sciences 57: 256 pp.
Radoman, P. (1985). Hydrobioidea, a Superfamily of Prosobranchia (Gastropoda) II. Origin, Zoogeography, Evolution in the Balkans and Asia Minor. Institute of Zoology, Belgrade.
Ryan, S. and Griffiths, H.I. (2001). The decline and probable extinction of Graecoanatolica macedonica (Gastropoda: Orientalinidae) in Balkan Lake Dorjan. J. Conchol., 37: 261-265.
Sahin, S. K., Koca, S. B., & Yildirim, M. Z. (2012). New genera anatolidamnicola and Sivasi (Gastropoda: Hydrobiidae) from Sivas and Malatya (Turkey). Acta Zoologica Bulgarica, 64(4): 341–346.
Saitou, N. and Nei, M. (1987). The Neighbor-joining Method: A New Method for Reconstructing Phylogenetic Trees’. Mol Biol Evol., 4(4): 406-425.
Schander, C. and Sundberg, P. (2001). Useful Characters in Gastropod Phylogeny: Soft Information or Hard Facts?. Systematıc Biology, 50(1): 136–141.
Schütt, H. (1962). Neue Süßwasser-Prosobranchier Griechenlands. Archiv für Molluskenkunde 91: 157-166.
Schütt, H. (1964). Die Molluskenfauna eines reliktiiren Quellsees der südlichen Türkei. − Arch. Moll., 93: 173-180.
Schütt, H. (1980). Zur Kenntnis griechischer Hydrobiiden. − Arch. Moll., 110: 115-149.
Schütt, H. (1990). Die pleistozinen Mollusken dreier pisidischer Salzseen. − Mitt. Dtsch. Malakozool. Ges. 46: 15-24.
Yildirim, Z. (1996). Türkiye Prosobranchia (Gastropoda: Mollusca) Türleri ve Zoocoğrafik Yayılışları 1. Tatlı ve Acısular. Turkish Journal of Zoology, 23: 877–900.
Wilke, T. and Falniowski, A. (2001). The genus Adriohydrobia (Hydrobiidae: Gastropoda): Polytypic species or polymorphic populations? J Zool Syst Evol Res., 39(4): 227-234.
Wilke, T., Davis, G.M., D Qiu, D. and Spear, R.C. (2006). Extreme Mitochondrial Sequence Diversity in The Intermediate Schistosomiasis Host Oncomelanıa Hupensıs Robertsonı: Another Case of Ancestral Polymorphism? Malacologia, 48(1–2): 143–157.