The relaxant responses induced by ethanol in the smooth muscle: role of extracellular and intracellular Ca

Alkol dünyada yaygın olarak tüketildiğinden, etanolün gastrointestinal sistem üzerindeki etkisinin mekanizmasının anlaşılması önemlidir. Bu çalışmada, izole fare mide fundusunda etanol ile indüklenen gevşemeler üzerinde ekstraselüler ve intraselüler kalsiyum (Ca2+)'un rolünü araştırmayı amaçladık. Bu çalışmada her iki cinsten (Swiss albino) fare kullanıldı. Fareler servikal dislokasyon ile öldürüldükten sonra longitudinal insizyon suretiyle gastrik fundal stripler hazırlandı ve içinde Tyrode solusyonu olan organ banyosuna 0.5 g tansiyon altında asıldı. Banyo ortamı 37 oC'de sabit tutuldu ve %95 O2 ve %5 CO2 ile gazlandırıldı. Deneysel veriler izometrik transdusır ile kaydedildi. Etanol(164 mM) izole fare mide fundus şeritlerinde tekrarlanabilir gevşemelere neden oldu. Bu gevşemeler L-tipi Ca2+ kanal blokörü verapamil (10-5-5x10-4 M) ve ryanodin reseptör (intraselüler kalsiyum kanal)'lerinin spesifik blokörü rutenyum red (10-5-10-4M) ile istatistiksel olarak anlamlı bir şekilde azaldı. Buna karşılık Ca2+-ATPaz'ın spesifik inhibitörü siklopiazonik asit (CPA; 10-6-10-5 M) bu gevşemeleri etkilemedi. Deneysel bulgular, izole fare mide fundus şeritlerinde etanol (164 mM) ile oluşturulan gevşemelerde hem ekstraselüer hem de intraselüler Ca2+'un rolü olabileceğini telkin etmektedir

Düz kasta etanol ile indüklenen gevşeme cevapları: ekstraselüler ve intraselüler Ca2+'un rolü

Since worldwide alcohol consumption, the understanding the mechanism of action of ethanol is important in the gastrointestinal tract. In this study we aimed to clarify the role of extracellular and intracellular calcium (Ca2+) on relaxations induced by ethanol in isolated mouse gastric fundus. Mice (Swiss albino) of either sex were used in this study. After killing the mice by cervical dislocation the gastric fundal strips were prepared by longitudinal incision and mounted under 0.5 g tension in an organ bath filled with Tyrode's solution. The bath medium was maintained at 37 oC and gassed with %95O2 and 5%CO2. Experimental data were recorded by an isometric transducer. Ethanol (164 mM) caused reproducible relaxations in isolated mouse gastric fundal strips. These relaxations were significantly inhibited by verapamil (10-5-5x10-4 M), a blocker of L-type Ca2+ channels, and ruthenium red (10-5-10-4 M), a blocker of ryanodine receptors (intracellular Ca2+ channels) in a concentration dependent manner. On the other hand cyclopiasonic acid (CPA; 10-6-10-5 M), a blocker of Ca2+-ATPase, failed to affect the relaxations induced by ethanol. The results of experimental data suggest that extracellular Ca2+ influx and intracellular Ca2+release may play a role on relaxations induced by ethanol in the isolated mouse gastric fundal smooth muscle

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