Zeyne HAN, Tamer ERDEM, Nasuhi Engin AYDIN

Sigara Dumanının Tavşan Kulak Kıkırdak Grefti Canlılık ve Bütünlüğüne Etkisi

Sigara dumanının tavşan kulak kıkırdak grefti canlılık ve bütünlüğü üzerine etkilerinin incelenmesi amaçlandı. Sigara grubunda 8 ve kontrol grubunda da 8 adet olmak üzere toplam 16 adet, beyaz Yeni Zellanda tavşanı sol kulaklarından 30x20 mm ebatlarında perikondriyumlu kıkırdak greftler elde edildi ve parçalara ayrıldı. Kıkırdaklar intakt, 1 kez ve 2 kez ezilmiş olarak her hayvanın kıkırdak grefti kendi paraspinal sırt bölgesinde sağ ve solda toplam 6 adet cebe yerleştirildi. Sigara grubu özel dizayn edilen kafeste günde 4 saat 12 hafta boyunca sigara dumanına maruz bırakıldı. 6. ve 12. haftalarda birer taraftaki greftler alınarak mikroskopik inceleme yapıldı. Mikroskopik olarak viabilite, skleroz, osteoid doku ve rejenerasyon değerlendirildi. Her parametre için, tesbit edilen bir skaladan puanlar verildi. Verilen puanlar istatistiksel olarak değerlendirildi.İstatistiksel olarak anlamlı fark viabilite açısından ezilmiş graftler ve intakt greftler araında siagara grubaunda viabilite azalması olarak bulundu. Deneysel tavşan modelimizde sigara dumanı maruziyetinde kıkırdak greftinin ezilerek kullanılmasının viabiliteyi azaltmasının farklı deneysel modellerlede incelenmesi yolunu açmakta ve klinik gözlemlerin yapılması için kanıt oluşturmaktadır kaanatindeyiz.

Effect of Cigarette Smoke on Rabbit Ear Cartılage Graft Viability and Integrity

The effect of cigarette smoking on cartilage grafts implanted to rabbit ear is investigated on New Zealand White rabbits. Eight animals for each experimental and control groups were maintained. Cartilage grafts were taken from the left ear and implanted as smaller pieces to paraspinal soft tissues bilaterally in each animal. Cartilage fragments were implanted as intact, crushed for once or twice to the six paraspinal soft tissue sites.The duration of the experiment was 12 weeks. One group was exposed to cigarette smoke in a special chamber for 4 hours a day continuously during this period. Cartilage grafts on one paraspinal site were examined microscopically. At the end of the 12th week the grafts on the other side were all excised and examined microscopically in respect to viability, sclerosis, osteoid formation and regeneration. Statistically significant differences were not found between the groups except intact and crushed grafts on the 12 week smoking model in regard to viability which was less in the crushed cartilages. Our finding in this experimental model suggests a negative effect of cigarette smoking on crushed cartilage grafts which opens a door to further experimental models and clinical observations.

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