Alparslan GÖKÇİMEN, Tuncay KULOĞLU, Yurdun KUYUCUSU, Sait POLAT, Tuğba ÇELİK SAMANCI, Murat BOYACIOĞLU

Yüksek Yağ İçerikli Diyetle Beslenmiş Sıçanların Karaciğer Dokularının Histokimyasal ve Biyokimyasal İncelenmesi

Amaç: Karaciğer yağlanması kronik karaciğer hastalıklarının en yaygın sebeplerinden birisidir ve son yıllarda oldukça artmıştır. Bu çalışmada yüksek yağ içerikli diyetle (HFD) sıçanlarda oluşturulan yağlanma modelinin karaciğer dokularındaki etkilerinin yapısal ve biyokimyasal olarak araştırılması amaçlanmıştır. Gereç ve Yöntemler: Çalışmada 16 adet Wistar albino cinsi erkek sıçan (160±30 g) kullanıldı. Sıçanlar rasgele iki gruba ayrıldı. Normal beslenen grup standart sıçan yemi ile ve HFD ile 10 hafta boyunca beslendi. Deney sonunda biyokimyasal ve yapısal incelemeler için karaciğer ve kalpten kan örnekleri alındı. Karaciğer dokusundan elde edilen kesitler ışık mikroskobu ve elektron mikroskobu ile incelendi ve iNOS enzim aktivitesini belirlemek amacıyla immünohistokimyasal olarak boyandı. Karaciğer dokularında malondialdehit (MDA), süperoksit dismutaz (SOD), CAT, glutatyon enzim aktiviteleri ve serum örneklerinde ALT, AST ve trigliserit (TG) seviyeleri ölçüldü. Bulgular: Işık mikroskobu ve elektron mikroskobik incelemeler sonucunda HFD diyetle beslenen grupta yağlanmaya sebep olan yapısal değişiklikler gözlendi. HFD grupta gözlenen ALT, AST ve TG değerlerindeki artış ile karaciğerde gözlenen yapısal hasar biyokimyasal olarak da doğrulandı. HFD grupta MDA aktivitesi artarken SOD ve CAT aktivitelerinde düşüş gözlendi. HFD grupta iNOS enzim aktivitesi de anlamlı şekilde arttı. Sonuç: Bu çalışmada 10 hafta boyunca yüksek yağ içerikli diyetle beslenen sıçanların karaciğer dokularında yağlanmanın geliştiği ve bu sürece inflamasyonunda etki etmesiyle birlikte oksidatif hasarın tetiklenip antioksidan savunma sisteminin devreye girdiği gözlendi

Biochemical and Histopathological Investigation of Liver Tissues on High Fat Diet Fed Rats

Objective: Hepatic steatosis is a common cause of liver disease and has become more prevalent recently. This study was conducted to investigate the biochemical and histopathological changes in the liver tissue high fat diet (HFD)-induced steatosis in rats. Materials and Methods: In this study, 16 male Wistar-albino rats (160±30 g in weight) were used and divided into two groups: The normal fed diet group fed with a standard diet and the HFD group fed with a HFD for 10 weeks. At the end of the experiment, the liver sections and blood from the heart underwent biochemical and histological investigations. The sections obtained from the liver were examined by light and electron-microscopy and the sections stained immunohistochemically to determine the iNOS activity. The antioxidant activities in liver samples and the alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) levels in serum were measured. Results: Because of light-microscopy and electron-microscopic investigations, histopathological changes caused the steatosis were observed in the HFD group. The histopathological damage observed in the liver was confirmed biochemically with the increase in the ALT, AST, and TG levels. While the malondialdehyde activity increased, the superoxide dismutase and catalase activities decreased in the HFD group. iNOS enzyme activity increased in the HFD group. Conclusion: In this study, it was observed that steatosis developed in the liver tissue of rats fed with the HFD at 10 weeks and oxidative damage was triggered with the influence of inflammation and activation of the antioxidant defense system.

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