Nil ÇULHACI, İbrahim METEOGLU, Emel ÜNAL, Esra ÖZKARA, Hedef ÖZGÜN, Nezih MEYDAN, Muhan ERKUS

KOLOREKTAL ADENOMLAR VE ADENOKARSİNOMLARDA HÜCRE SİKLUSUNU DÜZENLEYEN PROTEİNLERİN VE APOPİTOZLA İLGİLİ BELİRTEÇLERİN EKSPRESYONU

AMAÇ:Tümör gelişimi proliferasyon, büyümede durma ve hücre ölümü arasındaki denge ile düzenlenir. Buçalışmada, kolon karsinomunun gelişimindeki rollerinin değerlendirilmesinde p27, siklin D1, bcl-2 ve bcl-xexpresyonunu inceledik.GEREÇ ve YÖNTEM:p27, siklin D1, bcl-2 ve bcl-x expresyonunun düzeyleri, adenomlara komşu normalmukozada (n=30), adenomlarda (n=30), adenokarsinomlara komşu normal mukozada (n=63),adenokarsinomlarda (n=63) ve metastazlarda (n=16) immünohistokimyasal olarak incelendi. İmmün boyamaiçin standart streptavidin-biotin immünperoksidaz metodu kullanıldı ve boyanan slaytlar yarı kantitatif kriterlerkullanarak mikroskopik olarak incelendi.BULGULAR:Normal mukozada p27 protein ekspresyonu saptandı, adenokarsinomlar ise bu proteinekspresyonunda azalma gösterdi. p27 ekspresyonunda azalma tümör progresyonu ile ilişkili idi (p=0,026). SiklinD1 boyaması adenokarsinom ve metastazların çoğunda belirgindi (p=0,042). Bu arada, p27 ve siklin D1ekspresyonu arasında herhangi bir ilişki saptamadık. Bcl-2 ve bcl-x ekspresyonu da tümör progresyonundaistatistiksel olarak belirgin anlamlılık göstermedi.SONUÇ:Bu çalışmanın sonucu p27 ve siklin D1 protein düzeylerinin kolon kanserinin progresyonunundaönemli rolü olduğunu göstermektedir. Bcl-2 ve bcl-x ekspresyonunun ise rolü saptanmamıştır

Anahtar Kelimeler:

Kolon kanseri, hücre döngüsü, apopitoz

Kolorektal Adenomlar ve Adenokarsinomlarda Hücre Siklusunu Düzenleyen Proteinlerin ve Apopitozla Ilgili Belirteçlerin Ekspresyonu

OBJECTIVE: Tumor growth is regulated by a balance between proliferation, growth arrest and cell death. In this study, we have examined the expression of p27, cyclin D1, bcl-2, and bcl-x for evaluation of their roles in colon carcinoma progression. MATERIALS and METHODS: The levels of p27, cyclin D1, bcl-2, and bcl-x expression were examined by immunohistochemistry in transitional normal mucosa adjacent to adenomas (n=30), adenomas (n=30), transitional normal mucosa adjacent to adenocarcinomas (n=63), adenocarcinomas (n=63) and metastasis (n=16). Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria. RESULTS: Normal mucosa expressed p27 protein and adenocarcinomas displayed a decrease in the expression of this protein. Decreased expression of p27 was associated with tumor progression (p=0.026). Cyclin D1 staining was prominent in most of the adenocarcinomas and metastasis (p=0.042). Meanwhile, we could not find any relation between p27 and cyclin D1 expression. Bcl-2 and bcl-x expression also did not show any statistically significant correlation in tumor progression. CONCLUSION: The results of this study indicate that reduced p27 and cyclin D1 protein levels play an important role in progression of colon cancer. Bcl-2, and bcl-x expression were of no role.

Keywords:

Colon carcinoma, cell cycle, apoptosis,

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