Spectral Analysis of Sargassum ilicifolium (Turner) C. Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. Current research in drug discovery from medicinal plants involves the identification of safe and inexpensive lead molecule towards diseases. Cancer is a second major national burden leading to cause death. Reducing the nation’s cancer burden is a great challenge to win the “War of cancer”. During the last decades, numerous novel compounds have been found from marine organisms with interesting pharmaceutical activities. Hence, a novel brown algae, Sargassum ilicifolium (Turner) C.Agardh belonging to Sargassaceae family has selected and the study was aimed to investigate the in vitro anti-proliferative activity of ethanolic extract and chloroform fraction of Sargassum ilicifolium (Turner) C.Agardh. against colon cancer (HT-29) and lung cancer (A549) cell lines. Extraction of seaweed using 70% ethanol and fractionated with various solvents. Based on phytochemical screening, chloroform fraction was selected, then it was subjected to GC-MS analysis. The 70% ethanolic exract and chloroform fraction were selected to in vitro MTT cell line assay using colon cancer cell line (HT-29) and lung cancer cell line (A549). The GC-MS analysis report confirmed the presence of 21 compounds and in the the invitro assay, IC50value showed the effective anti-proliferative activity against colon and lung cancer cell lines. The results of the present study proved the anti-proliferative effect of Sargassum ilicifolium. Further, the active biomolecule in the fraction has to be isolated and charecterized the formulated product subjected to in vivo study to strengthen the anticancer activity.
___
Coseri S. Natural products and their analogues as efficientanticancer drugs. Mini Rev Med Chem 2009; 9:560-71.
Newman D. Natural products as leads to potential drugs: anold process or the new hope for drug discovery? J Med Chem2008; 51:2589-99.
Newman D, Cargg G, Snader K. Natural products as sourcesof new drugs over the period 1981-2002. J Nat Proud 2003;6:1022-37.
Prakash NS, Sundaram R, Mitra SK. In vitro and In vivo anticancer activity of Bacoside A from whole plant of Bacopamonnieiri (Linn). Am J Pharmacol Toxicol 2011;5:11-9.
Lahaye M, Kaeffer B. Seaweed dietary fibres: Structure, physicchemicaland biological properties relevant to intestinalphysiology. Sci Aliment 1997; 17:619-39.
Bhaskar N, Miyashita K. Lipid composition of Padinatetratomatica brown seaweed of the west cost of India. IndianJ Fish 2005;52:263-8.
Natarajan S,Shanmugiahthevar KP, Kasi PD. Cholinesteraseinhibitors from Sargassum and Gracilaria gracilis: Seaweedsinhibiting south Indian costal areas aslant, Gulf of Mannar.Nat Prod Res 2009;23:355-69.
Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, HongYK. Antipyretic, analgesic and anti-inflammatory activities ofseaweed Sagassum fulvellum and Sargassum thunbergi in mice.J Ethanopharmacol 2008;116:187-90.
Ruperez P, Saura-Calixto F. Dietary fiber and physiochemicalproperties of edible Spanish seaweeds. Eur Food Res Technol2001; 212:349-54.
Yan X, Nagata T, Fan X. Antioxidant activities in somecommon seaweeds. Plant Foods Hum Nutr 1998;52:253-62.
Sumithra M, Arunachalam G. Acute and subacute oral toxicitystudy of Sargassum ilicifolium Turner C. Agardh in rodents.Int J PharmTech Res 2014; 6: 821-8.
Mossman T. Rapid colorimetric assay for cellular growth andsurvival:application to proliferation and cytotoxicity assays. JImmunol Method. 1983; 65:55-63.
Lee KH, Xiao Z. Podophyllotoxin and analogs. In: Anticanceragents from natural products. Editors: Cragg GM, KingstonDGI, Newman DJ. CRC Press. 2005, pp. 71-88
Cartoni G, Ciardi M, Giarrusso A, Rosati F. Capillary gaschromatographic-mass spectrometric detection of anabolicsteroids. J Chromatogr 1983; 279:515–22.