Gholamreza ASGHARI, Mohamad AKBARI, Majid ASADI-SAMANI

Phytochemical analysis of some plants from Lamiaceae family frequently used in folk medicine in Aligudarz region of Lorestan province

Many of the plants from the Lamiaceae family have been traditionally used as medicine all over the world. Also, some of the secondary metabolites isolated from this family have shown interesting biological function. In this study we have analyzed phytochemicals of some plants from Lamiaceae family frequently used in folk medicine in Aligudarz region of Lorestan province. In this regard, the plant species were collected and systematically identified during 2014-2015. The traditional and local uses of collected plants were questioned through informed consent semi-structured interviews with local informants. Phytochemical analysis was conducted to test the presence of compounds such as alkaloids, flavonoids, saponins, tannins, anthraquinone and glycosides. 25 plant species belonging to 13 genes were collected and identified. The most uses of the plants were in treating cold, gastrointestinal disorders and as flavoring agents. From 25 plant species, a number of 23 species had tannin, 22 species exhibited positive reactions to flavonoids, 4 species showed positive reactions to alkaloids and 1 species exhibited positive reactions to saponins. This research has provided insights on the use of secondary metabolites in folk medicine for promotion of appropriate human health. The studied plants in this article can be seen as a potential source for discovering new drugs.

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Dalkic E, Wang X, Wright N, Chan C. Cancer-Drug Associations: A complex system. PLoS ONE 2010; 5: 1-15.
T.C Türkiye Halk Sağlığı Kurumu. Türkiye kanser istatistikleri http://kanser.gov.tr/Dosya/2017Haberler/2014-RAPOR.pdf (Erişim tarihi: 07 Şubat 2017).
Türkiye Halk Sağlığı Kurumu Kanser Daire Başkanlığı. Kanser istatistikleri http://kanser.gov.tr/ Dosya/2017Haberler/2017_4_subat.pdf (Erişim Tarihi: 07 Şubat 2017).
Türkiye Halk Sağlığı Kurumu Kanser Daire Başkanlığı. Kanser Tedavisi http://kanser.gov.tr/kanser/kanser-tedavisi/38- kanser-tedavisi.html (Erişim tarihi: 07 Şubat 2017).
Shepherd FA, Dancey J, Arnold A, Neville A, Rusthoven J, Johnson RD, Fisher B, Eisenhauer E. Phase II studyof pemetrexed disodium, a multitargeted antifolate, and cisplatin as first-line therapy in patients with advanced nonsmall cell lung carcinoma. Cancer 2001; 92: 595-600.
Istifli ES, Topaktas M. Genotoxicity of pemetrexed in human peripheral blood lymphocytes. Cytotechnology 2013; 65: 621- 8.
Eldin NE, Elnahas HM, Mahdy MAE, Ishida T. Liposomal pemetrexed: formulation, characterization and in vitro cytotoxicity studies for effective management of malignant pleural mesothelioma. Biol Pharm Bull 2015; 38: 461-9.
Chattopadhyay S, Moran RG, Goldman ID. Pemetrexed: biochemical and cellular pharmacology, mechanisms, and clinical applications. Mol Cancer Ther 2007; 6: 404-17.
Choudhury RC, Ghosh SK, Palo AK. Potential transmission of the cytogenetic toxic effects of methotrexate in the male germline cells of swiss mice. Environ Toxicol Pharmacol 2001; 10: 81-8.
Rojas E, Valverde M, Lopez MC, Naufal I , Sanchez I, Bizarro P, Lopez I, Fortoul TI, Wegman PO. Evaluation of DNA damage in exfoliated tear duct epithelial cells from individuals exposed to air pollution assessed by single cell gel electrophoresis assay. Mutat Res 2000; 468: 11-7.
Dusinska M, Collins AR. The comet in human biomonitoring: Gene–environment interactions. Mutagenesis 2008; 23: 191- 205.
Karlsson HL, Bucchianico SD, Collins AR, Dusinska M. Can the comet assay be used reliably to detect nanoparticleinduced genotoxicity? Environ Mol Mutagen 2015; 56: 82-96.
Villarini M, Moretti M, Pasquini R, Sforzolini GS, Fatigoni C, Marcarelli M, Monarca S, Rodrıgue AV. In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage (comet) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicol 1998; 130: 129-39.
Vandghanooni S, Eskandani M. Comet: a method to evaluate genotoxicity of nano-drug delivery system. BioImpacts 2011; 1: 87-97.
Nunes APM, Machado SCF, Nunes RM, Danta FJS, De Mattos JCP, de-Arau jo AC. Analysis of genotoxic potentiality of stevioside by comet. Food Chem Toxicol 2007; 45: 662-6.
Rozgaj R, Kasuba V, Brozovic G , Jazbec A. Genotoxic effects of anaesthetics in operating theatre personnel evaluated by the comet and micronucleus test. Int J Hyg Environ Health 2009; 212: 11-7.
Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel/comet: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000; 35: 206-21.
Szeto YT, Benzie IFF, Collins AR, Choi SW, Cheng CY, Yow CMN, Tse MMY. A buccal cell model comet assay: Development and evaluation for human biomonitoring and nutritional studies. Mutat Res 2005; 578: 371-81.
Wang Y, Zhao R, Goldman ID. Decreased expression of the reduced folate carrier and folypolyglutamate synthetase is the basis for acquired resistance to the pemetrexed antifolate (LY231514) in an L1210 murine leukemia cell line. Biochem Pharmacol 2003; 65: 1163-70.
Giovannetti E, Mey V, Danesi R, Mosca I, Tacca MD. Synergistic cytotoxicity and pharmacogenetics of gemcitabine and pemetrexed combination in pancreatic cancer cell lines. Clin Cancer Res 2004; 10: 2936-43.
Giovannetti E, Mey V, Nannizzi S, Pasqualetti G, Marini L, Tacca MD, Danesi R. Cellular and pharmacogenetics foundation of synergistic interaction of pemetrexed and gemcitabine in human non–small-cell lung cancer cells. Mol Pharmacol 2005; 68: 110-8.
23. Buqué A, Muhialdin JS, Muñoz A, Calvo B, Carrera S, Aresti U, Sancho A, Rubio I, Vivanco GL. Molecular mechanism implicated in Pemetrexed-induced apoptosis in human melanoma cells. Mol Cancer 2012; 11: 2-15.
Li T, Ling YH, Goldman D, Perez-Soler R. Scheduledependent cytotoxic synergism of pemetrexed and erlotinib in human nonsmall cell lung cancer cells. Clin Cancer Res 2007; 13: 3413-22.
Yang TY, Chang GC, Chen KC, Hung HW, Hsu KH, Wu CH, Sheu GT, Hsu SL. Pemetrexed induces both intrinsic and extrinsic apoptosis through ataxia telangiectasia mutated/ p53-dependent and -independent signaling pathways. Mol Carcinog 2013; 52: 183-94.
Dhawan A, Bajpayee M, Pandey AK, Parmar D. Protocol for the single cell gel electrophoresis/comet for rapid genotoxicity assessment. http://www.cometassayindia.org/protocol%20 for%20comet%20assay.pdf. Erişim tarihi : 20 February 2017. 26. Collins AR. The comet for DNA damage and repair. Mol Biotechnol 2004; 26: 249-61.
Shahin AA, Ismail MM, Saleh AM, Moustafa HA, Aboul-Ella AA, Gabr HM. Protective effect of folinic acid on low-dose methotrexate genotoxicity. Z Rheumatol 2001; 60: 63-8.
Padmanabhan S, Tripathi DN, Vikram A, Ramarao P, Jena GB. Methotrexate-induced cytotoxicity and genotoxicity in germ cells of mice: Intervention of folic and folinic acid. Mutat Res 2009; 673: 43-52.
Kalweit S, Vasudey V, Obe G. Liquid-holding experiments with human lymphocytes; III experiments with G0 and G1 cells. Mutat Res 1988; 207: 41-4.
Collins AR, Dobson VR, Dusinska M, Kennedy G, Stetina R. The comet assay: What can it really tell us?. Mutat Res 1997; 375: 183-93.
Comet Assay Interest Group. http://cometassay.com/index_ files/Page345.htm (Erişim tarihi 15 Şubat 2017).
Kastan MB. DNA damage responses: mechanisms and roles in human disease. Mol Cancer Res 2008; 6: 517-24.
McKenna DJ, McKeown SR, McKelvey-Martin VJ. Potential use of the comet in the clinical management of cancer. Mutagenesis 2008; 23: 183-90.