Pemetrekset’in in vitro Alkali Tek Hücre Jel Elektroforez Yöntemi ile Genotoksik Potansiyelinin Değerlendirilmesi

Pemetrekset, mezotelyoma, küçük hücreli olmayan akciğer kanseri ve pek çok neoplazmın tedavisinde kullanılan kemoterapötik bir ilaçtır. Kemoterapötik ilaçlar tedavide sadece kanser hücrelerini hedeflemezler. Tedavi süresince normal hücrelerde bu ilaçlarla karşılaşırlar. Buradan yola çıkılarak planlanan bu çalışmada, pemetrekset’in in vitro koşullarda nomal lökositler üzerine genotoksik etkisinin olup olmadığının ortaya konulması amaçlandı. Çalışmada, genotoksisite çalışmalarında hızlı ve güvenilir sonuçlar vermesi dolayısıyla sıklıkla kullanılan bir yöntem olan alkali tek hücre jel elektroforez yöntemi kullanıldı. İki gönüllü donörden alınan insan periferal kan örneklerinden elde edilen lökositler, ilacın dört farklı konsantrasyonu (25, 50, 75 ve 100µg/mL) ile 1 saat muamele edildi. DNA hasarı bakımından 75µg/mL (p˂0,05) ve 100µg/ml’de (p˂0,01) negatif kontrole göre istatistiksel olarak anlamlı bir artış tespit edildi. Bu bulgu, pemetrekset’in normal lökositler üzerine genotoksik potansiyelinin olabileceğini göstermektedir.

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Marmara Pharmaceutical Journal-Cover
  • ISSN: 1309-0801
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1985
  • Yayıncı: Marmara Üniversitesi
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