Mohammad-Taghi MORADI, Ali KARIMI, Zahra LORIGOOINI, Batoul POURGHEYSARI, Somayeh ALIDADI, Leila HASHEMI

In vitro anti influenza virus activity, antioxidant potential and total phenolic content of twelve Iranian medicinal plants

The emergence of medicine resistance strains of influenza A viruses to the chemical drogs lead to the development of alternative herbal compounds that inhibit the virus replication. Therefore, the aim of this research was to investigate in vitro anti-influenza A viruses activity, antioxidant potential, total phenolic, and flavonoid content of a total of 12 hydro alcoholic crude extracts obtained from 8 kinds of medicinal plants. Antiinfluenza A viruses activity of the extracts was investigated by the using of MDCK cell line and MTT (3-[4,5-dimethylthiazol– 2-yl]-2,5-diphenyltetrazolium bromide) method. Both 50% inhibitory concentration (IC50) and 50% cytotoxicity concentration (CC50) of the extracts were identified using regression analysis. The antioxidant activity, total phenol, and flavonoid content of the extracts were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu method, and aluminum chloride colorimetric method, respectively. The results demonstrated that there was high activity against influenza virus for Peganum harmala L., Equisetum arvense L., and Punica granatum L, extracts with IC50 value of 9.1 (CI95%:7.3-11.3), 6.45 (CI95%: 4.5-9.23), and 104.5 (CI95%: 82.8-131.8), respectively. DPPH radical scavenging activity showed that both Equisetum arvense L. and Punica granatum L. demonstrated the highest antioxidant activity with IC50 value of 6.5, 6.8 and 7.7 μg/mL, respectively. According to the results, some of these extracts might be further analyzed to develop effective anti-influenza factors.

PDF

___

Shayganni E, Bahmani M,, Asgary S, Rafieian-Kopaei M. Inflammaging and cardiovascular disease: Management by medicinal plants. Phytomedicine 2016;23:1119-26.

Famouri F, Salehi MM, Rostampour N, Hashemi E, Shahsanaee A. The effect of silymarin on nonalcoholic fatty liver disease of children. J Herbmed Pharmacol 2017;6:16-20.

Rouhi-Boroujeni H, Heidarian E, Rouhi-Boroujeni H, Deris F, Rafieian-Kopaei M. Medicinal plants with multiple effects on cardiovascular diseases: a systematic review. Curr Pharm Des 2017; 23: 999 –1015.

Sarrafchi A, Bahmani M, Shirzad H, Rafieian-Kopaei M. Oxidative stress and Parkinson’s disease: New hopes in treatment with herbal antioxidants. Curr Pharm Des 2016; 22: 238 – 46.

Shokri H, Sharifzadeh A. Zataria multiflora Boiss.: A review study on chemical composition, antifungal and anti-mycotoxin activities, and ultrastructural changes. J Herbmed Pharmacol 2017;6:1-9.

Lansky EP and Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol 2007; 109:177-206.

Moradi MT, Karimi A, Lorigooini Z. Alkaloids as the natural anti-influenza virus agents: A systematic review. Toxin Rev 2017:1-8.

Zeng X, Dong Y, Sheng G, Dong X, Sun X, Fu J. Isolation and structure determination of anti-influenza component from Mahonia bealei. J Ethnopharmacol 2006;108:317-9.

He J, Qi WB, Wang L, Tian J, Jiao PR, Liu GQ, Ye WC, Liao M. Amaryllidaceae alkaloids inhibit nuclear-to-cytoplasmic export of ribonucleoprotein (RNP) complex of highly pathogenic avian influenza virus H5N1. Influenza Other Respir Viruses 2013;7:922-31.

Mina CN, Farzaei MH, Gholamreza A. Medicinal properties of Peganum harmala L. in traditional Iranian medicine and modern phytotherapy: A review. J Tradit Chin Med 2015;35:104-9.

Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D. Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. Planta Med 2007;73:461-7.

Shirzad H, Taji F, Rafieian-Kopaei M. Correlation Between antioxidant activity of garlic extracts and WEHI-164 Fibrosarcoma tumor growth in BALB/c mice. J Med Food 2011;14:969-74.

Shirzad H, Taji F, Rafieian-Kopaei M. Correlation Between antioxidant activity of garlic extracts and WEHI-164 Fibrosarcoma tumor growth in BALB/c mice. J Med Food 2011;14:969-74.

Taghikhani A, Afrough H, Ansari-Samani R, Shahinfard N, Rafieian-Kopaei M. Assessing the toxic effects of hydroalcoholic extract of Stachys lavandulifolia Vahl on rat’s liver. Bratisl Med J 2014;115:121-4.

Mirhosseini M, Baradaran A, Rafieian-Kopaei M. Anethum graveolens and hyperlipidemia: A randomized clinical trial. J Res Med Sci 2014;19:758-61.

Heidarian E, Rafieian-Kopaei M. Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat. Pharm Biol 2013;51:1104-9.

Rabiei Z, Rafieian-kopaei M, Heidarian E, Saghaei E, Mokhtari S. Effects of Zizyphus jujube extract on memory and learning impairment induced by bilateral electric lesions of the nucleus basalis of Meynert in rat. Neurochem Res 2014;39:353-60.

Akhlaghi M, Shabanian G, Rafieian-Kopaei M, Parvin N, Saadat M, Akhlaghi M. Citrus aurantium blossom and preoperative anxiety. Rev Bras Anestesiol 2011;61:702-12.

Rahnama S, Rabiei Z, Alibabaei Z, Mokhtari S, Rafieian-kopaei M, Deris F. Anti-amnesic activity of Citrus aurantium flowers extract against scopolamine-induced memory impairments in rats. Neurol Sci 2015;36:553-60.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Visintini Jaime MF, Redko F, Muschietti LV, Campos RH, Martino VS, Cavallaro LV. In vitro antiviral activity of plant extracts from Asteraceae medicinal plants. Virol J 2013;10:245.

Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Epidemiol 1938;27:493-7.

WHO, Manual for the Laboratory Diagnosis and Virological Surveillance of Influenza. Global Influenza Surveillance and Response System (GISRS) 2011. http://www.who.int/ influenza/gisrs_laboratory/manual_diagnosis_surveillance_ influenza/en/ (accessed May 18, 2016).

Kim Y, Narayanan S, Chang KO. Inhibition of influenza virus replication by plant-derived isoquercetin. Antiviral Res 2010;88:227-35.

Karimi A, Mohammadi-Kamalabadi M, Rafieian-Kopaei M, Amjad L, Salimzadeh L. Determination of antioxidant activity, phenolic contents and antiviral potential of methanol extract of Euphorbia spinidens Bornm (Euphorbiaceae). Trop J Pharm Res 2016;15:759-64.

Asadi-Samani M, Bahmani M, Rafieian-Kopaei M. The chemical composition, botanical characteristic and biological activities of Borago officinalis: A review. Asian Pac J Trop Med 2014;7:S22-S8.

Karimi A, Moradi M-T. Total phenolic compounds and in vitro antioxidant potential of crude methanol extract and the correspond fractions of Quercus brantii L. acorn. J Herbmed Pharmacol 2015;4:35-9.

Moon J-H, Terao J. Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. J Agric Food Chem 1998;46:5062-5. 18.

Rafieian-Kopaei M, Shahinfard N, Rouhi-Boroujeni H, Gharipour M, Darvishzadeh-Boroujeni P. Effects of Ferulago angulata extract on serum lipids and lipid peroxidation. Evid Based Complement Alternat Med 2014;2014:680856.

Boiko YuA, Kravchenko IA, Shandra AA, Boiko IA. Extraction, identification and anti-inflammatory activity of carotenoids out of Capsicum anuum L. J Herbmed Pharmacol 2017;6:1015

Moradi MT, Rafieian-Kopaei M, Karimi A. A review study on the effect of Iranian herbal medicine against in vitro replication of herpes simplex virus. Avicenna J Phytomed 2016;6:506-15.

Karimi A, Rafieian-Kopaei M, Moradi MT, Alidadi S. AntiHerpes Simplex Virus Type-1 activity and phenolic content of crude ethanol extract and four corresponding fractions of Quercus brantii L Acorn. J Evid Based Complementary Altern Med 2017;22:455-61.

Karimi A, Moradi MT, Alidadi S, Hashemi L. Anti-adenovirus activity, antioxidant potential, and phenolic content of black tea (Camellia sinensis Kuntze) extract. J Complement Integr Med 2016;13:357-63.

Jivad N, Asadi-Samani M, Moradi M. The most important medicinal plants effective on migraine: A review of ethnobotanical studies in Iran. Der Pharm Chem 2016;8:4626.

Kooti W, Ghasemiboroon M, Asadi-Samani M, Ahangarpoor A, Abadi MNA, Afrisham R, Dashti N. The effects of hydroalcoholic extract of celery on lipid profile of rats fed a high fat diet. Adv Environ Biol 2014;9:325-31.

Parsaei P, Bahmani M, Karimi M, Naghdi N, Asadi-Samani M, Rafieian-Kopaei M. A review of analgesic medicinal plants in Iran. Der Pharm Lett 2016;8:43-51.

Mirhoseini M, Moradi MT, Asadi-Samani M. Traditionally used medicinal plants in the treatment of kidney stone: A review on ethnobotanical studies in Iran. Ambient Sci 2016;3:16-21.

Moradi MT, Karimi A, Alidadi S. In vitro antiproliferative and apoptosis-inducing activities of crude ethyle alcohole extract of Quercus brantii L. acorn and subsequent fractions. Chin J Nat Med 2016;14:196-202.

Moradi MT, Rafieian-Koupaei M, Shahrani M. The effect of garlic methanol extract on gastric acid and pepsin in basic and stimulated conditions by electrical stimulus of vagus nerve in rats. Life Sci J 2013;10:99-104.

Dapat C, Kondo H, Dapat IC, Baranovich T, Suzuki Y, Shobugawa Y, Saito K, Saito R, Suzuki H. Neuraminidase inhibitor susceptibility profile of pandemic and seasonal influenza viruses during the 2009-2010 and 2010-2011 influenza seasons in Japan. Antiviral Res 2013;99:261-9.

van der Vries E, Schutten M, Fraaij P, Boucher C, Osterhaus A. Influenza virus resistance to antiviral therapy. Adv Pharmacol 2013;67:217-465.

Cannell JJ, Zasloff M, Garland CF, Scragg R, Giovannucci E. On the epidemiology of influenza. Virol J 2008;5:422X-5.

Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, Garten RJ, Gubareva LV, Xu X, Bridges CB, Uyeki TM. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 2009;360:2605-15.

Nicholson KG, Wood JM, Zambon M. Influenza. Lancet 2003;362:1733-45.