Nidal JARADAT, Abdel Naser ZAID, Eyass Zuhair ZAGHAL

Anti-lipase activity for Portulaca oleracea, Urtica urens, Brassica napus and Lathyrus hierosolymitanus wild plants from Palestine

Plants are used to the treatment and prevention of many of metabolic, degenerative and cardiovascular diseases. Obesity is one of the most common metabolic diseases which is considered one of the global health problems. The present study aimed to screen anti-lipase effect of Portulaca oleracea, Urtica urens, Brassica napus and Lathyrus hierosolymitanus traditional Palestinian medicinal wild plants. Anti-lipase activity was measured by using porcine pancreatic lipase inhibitory test which was established by using the UV-visible spectrophotometer method, while orlistat (anti-obesity drug) was used as a positive control reference. The porcine pancreatic lipase inhibitory effect for organic and aqueous extracts of Urtica urens were 157 μg/ml and 157.1 μg/ml, respectively. While the anti-lipase IC50 value for the organic extract of P. oleracea was 262.03 μg/ml. In addition, the aqueous extracts of Brassica napus and Portulaca oleracea have anti-lipase activity with the IC50 values 296.87 μg/ml and 417.62 μg/ml, respectively. Meanwhile, Lathyrus hierosolymitanus both aqueous and organic extracts were almost inactive. The results showed that Urtica urens, Portulaca oleracea, and Brassica napus have anti-lipase activity, which provided evidence for their folkloric use as functional food and medicine. These three plants could be used as antilipase agents in the pharmaceutical and nutritional industries or may be used as fatty food additives in order to decrease the absorption and digestion of fats from food.

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Kim YS, Lee Y, Kim J, Sohn E, Kim CS, Lee YM, Jo K, Shin S, Song Y, Kim JH, Kim JS. Inhibitory activities of Cudrania tricuspidata leaves on pancreatic lipase in vitro and lipolysis in vivo. Evid Based Complementary Altern Med 2012; 2012: 1-6.

Gholamhoseinian A, Shahouzehi B, Sharifi-Far F. Inhibitory effect of some plant extracts on pancreatic lipase. IJP-Inter J Pharmacol 2010; 6: 18-24.

Morikawa K, Ikeda C, Nonaka M, Suzuki I. Growth arrest and apoptosis induced by quercetin is not linked to adipogenic conversion of human preadipocytes. Metabolism 2007; 56: 1656-65.

Yun JW. Possible anti-obesity therapeutics from nature–A review. Phytochemistry 2010; 71: 1625-41.

De la Garza AL, Milagro FI, Boque N, Campión J, Martínez JA. Natural inhibitors of pancreatic lipase as new players in obesity treatment. Planta Med 2011; 77: 773-85.

Krentz AJ, Fujioka K, Hompesch M. Evolution of pharmacological obesity treatments: focus on adverse sideeffect profiles. Diabetes Obes Metab 2016; 18: 558-70.

Nuutila AM, Puupponen-Pimiä R, Aarni M, OksmanCaldentey K-M. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem 2003; 81: 485-93.

Piper AJ. Obesity hypoventilation syndrome-the big and the breathless. Sleep Med Rev 2011; 15: 79-89.

Marinou K, Tousoulis D, Antonopoulos AS, Stefanadi E, Stefanadis C. Obesity and cardiovascular disease: From pathophysiology to risk stratification. Int J Cardiol 2010; 138:3-8.

Popkin BM. The world is fat: The fads, trends, policies, and products that are fattening the human race. Penguin group, New York. 2009.

Jaradat NA, Zaid AN, Hussein F. Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine. Chem Biol Technol Agr 2016; 3: 31-7.

Zheng C-D, Duan Y-Q, Gao J-M, Ruan Z-G. Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs. J Chin Med Assoc 2010; 73: 319-24.

Bustanji Y, Issa A, Mohammad M, Hudaib M, Tawah K, Alkhatib H, Almasri I, Al-Khalidi B. Inhibition of hormone sensitive lipase and pancreatic lipase by Rosmarinus officinalis extract and selected phenolic constituents. J Med Plant Res 2010; 4: 2235-42.

Ranabahu P, Harborne JB. The flavonoids of the genus Lathyrus and a comparison of flavonoid patterns within the tribe vicieae. Biochem Syst Ecol 1993; 21: 715-22.

Syouf MQ. Lathyrus genetic resources in Asia. Natural distribution and utilization of Lathyrus in Jordan. International Plant Genetic Resources Institute, Washington. 1996, pp 6781.

White R. International Legume Database & Information Service. ILDIS, UK. 2005.

Johns T, Kitts W, Newsome F, Towers GN. Anti-reproductive and other medicinal effects of Tropaeolum tuberosum. J Ethnopharmacol 1982; 5: 149-61.

Mhatre SV. Pancreatic lipase inhibitor from food plant: Potential molecule for development of safe anti-obesity drug. GMG J Med Sci 2013; 3: 34-41.

Batista C, Barros L, Carvalho AM, Ferreira IC. Nutritional and nutraceutical potential of rape (Brassica napus L. var. napus) and “tronchuda” cabbage (Brassica oleraceae L. var. costata) inflorescences. Food Chem Toxicol 2011; 49: 1208-14.

Ali-Shtayeh MS, Jamous RM, Al-Shafie’ JH, Elgharabah WA, Kherfan FA, Qarariah KH, Khdair IS, Soos IM, Musleh AA, Isa BA, Herzallah HM, Khlaif RB, Aiash SM, Swaiti GM, Abuzahra MA, Haj-Ali MM, Saifi NA, Azem HK, Nasrallah HA.Traditional knowledge of wild edible plants used in Palestine (Northern West Bank): a comparative study. J Ethnobiol Ethnomed 2008; 4: 1-13.

Rahmatullah M, Ishika T, Rahman M, Swarna A, Khan T, Monalisa MN, Seraj S, Mou SM, Mahal MJ, Biswas KR. Plants prescribed for both preventive and therapeutic purposes by the traditional healers of the Bede community residing by the Turag River, Dhaka district. Am-Eurasian J Sustain Agric 2011; 5: 325-31.

Lim TK. Edible medicinal and non-medicinal plants. Springer, USA. 2012.

Parnell J, Doogue D, Webb DA. Webb’s an Irish flora. Cork University Press, Ireland. 2012.

Raman H, Raman R, Eckermann P, Coombes N, Manoli S, Zou X, Edwards D, Meng J, Prangnell R, Stiller J, Batley J, Luckett D, Wratten N, Dennis E.Genetic and physical mapping of flowering time loci in canola (Brassica napus L.). Theor Appl Genet 2013; 126: 119-32.

Ozkarsli M, Sevim H, Sen A. In vivo effects of Urtica urens (dwarf nettle) on the expression of CYP1A in control and 3-methylcholanthrene-exposed rats. Xenobiotica 2008; 38: 48-61.

Jimoh F, Adedapo A, Aliero A, Afolayan A. Polyphenolic and biological activities of leaves extracts of Argemone subfusiformis (Papaveraceae) and Urtica urens (Urticaceae). Rev Biol Trop 2010; 58: 1517-31.

Marrassini C, Acevedo C, Miño J, Ferraro G, Gorzalczany S. Evaluation of antinociceptive, antinflammatory activities and phytochemical analysis of aerial parts of Urtica urens L. Phytother Res 2010; 24: 1807-12.

Tuzlacı E, Aymaz PE. Turkish folk medicinal plants. Fitoterapia 2001; 72: 323-43.

Ali-Shtayeh M, Jamous RM. Traditional arabic Palestinian herbal medicine, TAPHM. Palestine: Biodiversity and Environmental Research Center, BERC; 2008.

Hussein MA. Purslane extract effects on obesity-induced diabetic rats fed a high-fat diet. Malays J Nutr 2010; 16: 41929.

Kotb FT. Medicinal plants in Libya. Arab encyclopedia house, Tripoli. 1985.

Rashed A, Afifi F, Disi A. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L.(growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol 2003; 88: 131-6.

Zar Kalai F, Han J, Ksouri R, El Omri A, Abdelly C, Isoda H. Antiobesity effects of an edible halophyte Nitraria retusa forssk in 3T3-L1 preadipocyte differentiation and in C57B6J/L mice fed a high fat diet-induced obesity. Evid Based Complementary Altern Med 2013; 2013:1-7.

Castro M, Preto M, Vasconcelos V, Urbatzka R. Obesity: The metabolic disease, advances on drug discovery and natural product research. Curr Top Med Chem 2016;16: 2577-604.

Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: Unexplored potential. Drug Discov Today 2007; 12: 879-89.

Saravanan G, Ponmurugan P, Deepa MA, Senthilkumar B. Anti‐obesity action of gingerol: Effect on lipid profile, insulin, leptin, amylase, and lipase in male obese rats induced by a high‐fat diet. J Sci Food Agr 2014; 94: 2972-7.

Williams DJ, Edwards D, Hamernig I, Jian L. Vegetables containing phytochemicals with potential anti-obesity properties: A review. Food Res Int 2013; 52: 323-33.

Tapsell L, Batterham M, Thorne R, O’shea J, Grafenauer SJ, Probst Y. Weight loss effects from vegetable intake: A 12-month randomised controlled trial. Eur J Clin Nutr 2014; 68: 778-85.

Jaradat N, Zaid AN, Hussein F, Ali I. The effects of preservation methods of grapevine leaves on total phenols, total flavonoids, and antioxidant activity. Marmara Pharm J 2017;21: 291-7.

James PT, Leach R, Kalamara E, Shayeghi M. The worldwide obesity epidemic. Obes Res 2001;9:228-33.