Mastura IBRAHIM, Saripah Salbiah SYED ABDUL AZZIZ, Chee Fah WONG, Wan Nor Izzati WAN MOHAMAD DIN, Wan Rusmawati WAN MAHAMOD, Yuhanis MHD BAKRI, Mohamad Syahrizal AHMAD, Rozita YAHAYA, Nor Hadiani ISMAIL, Wan Mohd Nuzul Hakimi Wan SALLEH

Evaluation of anti-lipase activity of leaf and bark extracts from Aquilaria subintegra and A. malaccensis

Obesity is one of the serious health condition in the world and contributes to many chronic illnesses augmenting to high risk of death rate. With the purpose of obesity treatment, natural sources come to be significant to replace commercial drugs due to their harmful effect to the body. Therefore, a lot of studies were conducted on natural sources due to their potential as medicinal herbs. Aquilaria sp. plants are well known in traditional medicine as a sedative, analgesic and digestive. Even though there were no reports on applications of Aquilaria sp. towards obesity treatment, this plant has traditionally been used as laxative for weight reducing. In this study, the anti-lipase activity of an extract of barks and leaves from A. subintegra and A. malaccensis were investigated. The anti-lipase activity was measured by colorimetric assay on pancreatic lipase activity using porcine pancreatic lipase (PPL; triacylglycerol lipase, EC 3.1.1.3). The result indicated that among these two species of Aquilaria, A. malaccensis bark in dichloromethane crude showed high anti-lipase activity. Thus, these results suggest that Aquilaria sp. plant extracts might be of therapeutic interest with respect to the treatment.

PDF

___

[1] Adnyana IK, Sukandar EY, Yuniarto ARI, Finna S. Antiobesity effect of the pomegranate leaves ethanol extract (Punicagranatum L.) In high-fat diet induced mice. Int J Pharmacy Pharm Sci. 2014; 6 (4): 4-9.

[2] Kazmi I, Afzal M, Rahman S, Iqbal M, Imam F, Anwar F. Antiobesity potential of ursolic acid stearoyl glucoside by inhibiting pancreatic lipase. Euro J Pharmacol. 2013; 709 (1- 3): 28-36.

[3] Sergent T, Vanderstraeten J, Winand J, Beguin P, Schneider Y. Phenolic compounds and plant extracts as potential natural anti-obesity substances. Food Chem. 2012; 135: 68-73.

[4] Yun JW. Phytochemistry Possible anti-obesity therapeutics from nature-A review. Phytochemistry 2010; 71 (14-15): 1625-1641.

[5] Nicolás-vázquez I, Hinojosa J, Cruz J, Miranda R, Aceveshernández JM. Orlistat interaction with sibutramine and carnitine . A physicochemical and theoretical study. J Mol Struct. 2014; 1062: 1-12.

[6] Padwal RS, Majumdar SR. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet. 2007; 369: 71- 77.

[7] Yunus S, Md Zaki NA, Ku Hamid KH. Microwave drying characteristics and antidiabetic properties of Aquilaria subintegra and Aquilaria malaccensis leaves. Adv Mat Res. 2015; 1113: 352-357.

[8] Hashim YZHY, Phirdaous A, Azura A. Screening of anticancer activity from agarwood essential oil. Phcog Res. 2014; 6 (3): 191-194.

[9] Noor N, Nik A, Adila N, Omar M, Ibrahim NA. In vitro antioxidant activity and phytochemical screening of Aquilaria malaccensis leaf extracts. J Chem Pharm Res. 2014; 6 (12): 688-693.

[10] Ito T, Kakino M, Tazawa S, Watarai T, Oyama M, Maruyama H, Araki Y, Hara H, Iinuma M. Quantification of polyphenols and pharmacological analysis of water and ethanol-based extracts of cultivated agarwood leaves. J Nutr Sci Vitaminol 2012; 58: 136-142.

[11] Ito T, Kakino M, Tazawa S, Oyama M, Maruyama H, Araki Y, Hara H, Inuma M. Identification of phenolic compounds in Aquilaria crassna leaves via Liquid ChromatographyElectrospray Ionization Mass Spectroscopy. J Food Sci Technol. 2012; 18 (2): 259-262.

[12] Kakino M, Tazawa S, Maruyama H, Tsuruma K, Araki Y, Shimazawa M, Hara H. Laxative effects of agarwood on lowfiber diet-induced constipation in rats. BMC Complement Altern Med. 2010; 10 (1): 68.

[13] Kamonwannasit S, Nantapong N, Kumkrai P, Luecha P, Kupittayanant S. Antibacterial activity of Aquilaria crassna leaf extract against Staphylococcus epidermidis by disruption of cell wall. Annal Clin Microb Antimicrob 2013; 12 (1): 1

[14] Jok VA, Che Radzi N, Ku Hamid KH. A review: Pharmacological properties of Aquilaria spp. Adv Mat Res. 2015; 1113: 193-197.

[15] Kwon DY, Rhee JS. A simple and rapid colorimetric method for determination of free fatty acids for lipase assay. J Am Oil Chem Soc. 1986; 63 (1): 89-92.

[16] Leow TC, Rahman RNZRA, Basri M, Salleh AB. High level expression of thermostable lipase from Geobacillus sp. strain T1. Biosci Biotech Biochem. 2004; 68 (1): 96-103.

[17] Wang L, Yamasaki M, Katsube T, Sun X, Yamasaki Y, Shiwaku K. Antiobesity effect of polyphenolic compounds from molokheiya (Corchorus olitorius L.) leaves in LDL receptordeficient mice. Eur J Nutr 2011; 50 (2): 127-133.

[18] Garza AL, Milagro FI, Boque N, Campion J, Martinez JA. Natural inhibitors of pancreatic lipase as new players in obesity treatment. Planta Med. 2011; 773-785.

[19] Huda AWN, Munira MAS, Fitrya SD, Salmah M. Antioxidant activity of Aquilaria malaccensis (Thymelaeaceae) leaves. Phcog Res. 2009; 1 (5): 270-273.

[20] Hara H, Ise Y, Morimoto N, Shimazawa M, Ichihashi K, Ohyama M, Iinuma M. Laxative effect of Agarwood leaves and its mechanism. Biosci Biotech Biochem. 2008; 72 (2): 335-345.