Esra EROĞLU ÖZKAN, Serpil Demirci KAYIRAN, Turgut TAŞKIN, Mahmoud ABUDAYYAK

In vitro antioxidant and cytotoxic activity of Muscari neglectum growing in Turkey

Muscari species have been used as antirheumatic, stomachic, diuretic, expectorant and anti verruca in folk medicine. The species have also been used as food for humans and animals in Turkey. In this study, the antioxidant and cytotoxic activities of different extracts obtained from Muscari neglectum were studied. The antioxidant capacities of the extracts were evaluated with DPPH free radical scavenging activity, metal chelating capacity and ABTS cation radical scavenging capacity assays. The cytotoxic potentials of the extracts were determined by MTT and LDH assays on HeLa-cells (human cancer cell line) and NRK-52E-cells (rat kidney cell line). The aqueous extracts from M. neglectum bulbs (IC50: 2.83± 0.54 mg/mL) and herba (IC50: 8.52± 1.3 mg/mL) exhibited stronger DPPH free radical scavenging activity than other extracts, respectively. The ethanol extract from M. neglectum bulbs showed the highest ABTS (27.88 %) cation radical scavenging activity. According to the metal chelating activity test results, the aqueous extracts from M. neglectum herba and bulbs showed the strongest metal chelating activity (28.99% and 28.07%, respectively).

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[1] Speta F. Hyacinthaceae. In: Kubitzki K, editor. Flowering Plants · Monocotyledons. The Families and Genera of Vascular Plants. 3. Berlin, Heidelberg: Springer; 1998.

[2] Demirci S, Özhatay N, Koçyiğit M. Muscari erdalii (Asparagaceae, Scilloideae), a new species from Southern Turkey. Phytotaxa. 2013;154(1):38-46.

[3] Yildirim H. Muscari elmasii sp. nova (Asparagaceae): a new species from western Anatolia, Turkey. Turk J Bot. 2016;40(4):380-387.

[4] Erol M, Tuzlacı E. Eğirdir (Isparta) yöresinin geleneksel halk ilacı olarak kullanılan bitkileri. XI BİHAT. 1995:22-24.

[5] Ugurlu E, Secmen O. Medicinal plants popularly used in the villages of Yunt Mountain (Manisa-Turkey). Fitoterapia. 2008;79(2):126-131.

[6] Lim T. Muscari neglectum. Edible medicinal and nonmedicinal plants: Springer; 2014. p. 122-5.

[7] Mahboubi M, Taghizadeh M. The antimicrobial and antioxidant activity of Muscari neglectum flower ethanol extract. Herba Pol. 2016;62(4):39-48.

[8] Usher G. A dictionary of plants used by man: Constable and Company Ltd.; 1974.

[9] Mulholland DA, Schwikkard SL, Crouch NR. The chemistry and biological activity of the Hyacinthaceae. Nat Prod Rep. 2013;30(9):1165-1210.

[10] Kayıran SD, Özkan EE. The ethnobotanical uses of Hyacinthaceae species growing in Turkey and a review of pharmacological activities. IJTK. 2017; 16(2): 243-250.

[11] Taskin T, Bitis L. In vitro antioxidant activity of eight wild edible plants in Bursa province of Turkey. Farmacia 2016;64(5): 706-711.

[12] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, RiceEvans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231-1237.

[13] Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys. 1994;315(1):161-169.

[14] Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbott BJ, Mayo JG, Shoemaker RH, Boyd MR. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988;48(3):589-601.

[15] Mayo JG, Shoemaker RH, Boyd MR. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988;48(3):589-601.

[16] Legrand C, Bour J, Jacob C, Capiaumont J, Martial A, Marc A, Wudtke M, Kretzmer G, Demangel C, Duval D. Lactate dehydrogenase (LDH) activity of the number of dead cells in the medium of cultured eukaryotic cells as marker. J Biotechnol. 1992;25(3):231-243.